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pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e

Plasmid

#90011

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Cdc25C S216A
NBirA*-14-3-3e

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Ser 216 to Ala

Promoter

CMV

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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ArpC2_pLib

Plasmid

#173679

Purpose

ArpC2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

ArpC2 (ARPC2 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2-N pc4789

Plasmid

#229753

Purpose

Bacterial expression plasmid of MBD2-N (N-terminus of MBD2: aa 1-152). C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

N-terminus of MBD2: aa 1-152

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2-MBDTRD pc4791

Plasmid

#229754

Purpose

Bacterial expression vector of MBD2-MBDTRD domains (aa 153-235) C-terminal tagged to the intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

MBD2-MBDTRD domains (aa 153-235)

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2∆C pc4792

Plasmid

#229755

Purpose

Bacterial expression plasmid of MBD2∆C lacking the C-terminus (a 236-414). C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

MBD2 lacking the C-terminus (a 236-414)

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2-C pc4793

Plasmid

#229756

Purpose

Bacterial expression plasmid of MBD2-C (C-terminus of MBD2: aa 236-414). C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

C-terminus of MBD2: aa 236-414

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pmMBD2∆C-G pc4794

Plasmid

#229757

Purpose

Mammalian expression vector of MBD2. The Protein is lacking the C-terminus: aa 236-414. C-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

MBD2 lacking the C-terminus: aa 236-414.

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2∆GR pc5078

Plasmid

#229758

Purpose

Bacterial expression plasmid of MBD2∆GR. The Protein is lacking the glycine/arginine rich domain: aa 75-96. C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

MBD2 lacking the glycine/arginine rich domain: aa…

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2a∆CC pc5081

Plasmid

#229760

Purpose

Bacterial expression vector of MBD2a lacking the coiled coil domain: aa 363-396.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Mammalian

Mutation

MBD2a lacking the coiled coil domain: aa 363-396.

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pmMBD2∆N∆CC-G pc5089

Plasmid

#229765

Purpose

Mammalian expression plasmid of MBD2∆N∆CC. Protein is lacking the coiled coil domain: aa 363-396 and the N-terminus: aa 1-152. C-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pmMBD2-C∆CC-G pc5091

Plasmid

#229766

Purpose

Mammalian expression plasmid of MBD2-C∆CC. C-terminus of MBD2 lacking the coiled coil domain: aa 363-396. C-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMBD2-1-GFP pc2067

Plasmid

#229559

Purpose

Mammalian expression vector codes for MBD2(aa 1-152) fused c-terminal to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

MBD2(aa 1-152)

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pmMBD2.2-GFP pc2068

Plasmid

#229560

Purpose

Mammalian expression vector codes for MBD2(aa 153-414) fused c-terminal to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

MBD2(aa 153-414)

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pmMBD2.4G pc2841

Plasmid

#229561

Purpose

Mammalian expression vector codes for MBD2(aa 235-414) fused c-terminal to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Bacterial

Mutation

MBD2(aa 235-414)

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pmMBD2-6G pc2843

Plasmid

#229562

Purpose

Mammalian expression vector codes for MBD2(aa 153-235) fused c-terminal to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Bacterial

Mutation

MBD2(aa 153-235)

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD1∆CxxC3 pc4783

Plasmid

#229748

Purpose

Bacterial expression vector of MBD1 with a deletion of CXXC3 domain. C-terminal tagged to the intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD1 (Mbd1 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

MBD1 lacking the third CXXC domain

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD4 pc4785

Plasmid

#229750

Purpose

Bacterial expression vector of MBD4 C-terminal tagged to the intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD4 (Mbd4 Mouse)

Tags

Intein

Expression

Bacterial

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2∆N(MBD2b) pc4787

Plasmid

#229752

Purpose

Bacterial expression plasmid of MBD2∆N(MBD2b) lacking the N-terminus: aa 1-152. C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

MBD2 lacking the N-terminus: aa 1-152.

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoED solvvol

Plasmid

#232958

Purpose

Galactose iduced expression of Gcn4 LVtoED solvvol in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED solvvol

Tags

TEV cleavage site

Expression

Yeast

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR36(DjCas13d-SapI)-CMV-intron-MCS-pA

Plasmid

#233031

Purpose

To express a DjCas13d-compatible gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d-compatible gRNA expression cassette

Use

AAV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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