We narrowed to 18,510 results for: puro

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pLenti-EML4-ALK variant 1

Plasmid

#183828

Purpose

Express EML4-ALK fusion variant 1 (E13;A20) in mammalian cells

Depositor

Aaron Hata

Article

Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

Inserts

ALK Receptor Tyrosine Kinase (ALK Human)
EMAP Like 4 (EML4 Human)

Use

Lentiviral

Tags

The fusion protein of EML4 exon 1-13 and ALK exon…

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307-SARS-CoV-2-3CL

Plasmid

#160278

Purpose

Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLX301-ZIM3-KRAB-PYL1

Plasmid

#154761

Purpose

Expresses ZIM3 KRAB domain fused to PYL1

Depositor

Mikko Taipale

Article

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Promoter

CMV

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMaCTag-P07

Plasmid

#124788

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX_TRE3G_DD::mCherry

Plasmid

#108679

Purpose

Tunable system for controlled gene expression and protein stability in mammalian cells

Depositor

Lucia Marucci

Article

Pedone et al Nat Commun. 2019 Oct 2;10(1):4481. doi:

Insert

Destabilizing Domain (DD) mCherry fusion protein

Use

Lentiviral

Tags

DD::mCherry fusion protein

Expression

Mammalian

Promoter

Doxycycline inducible

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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MED12_pLX307

Plasmid

#98350

Purpose

Lentiviral expression of MED12

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

MED12 (MED12 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

R1392Q

Promoter

E1Fa

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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hCRISPRi-v2, Cancer and Apoptosis (h2), top 5 sgRNAs/gene

Pooled Library

#83972

Purpose

Human CRISPRi Pooled Library targeting cancer and apoptosis genes

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only
OPA1-isoform1

Plasmid

#70173

Purpose

Mammalian expression of hsOPA1 isoform 1

Depositor

David Chan

Article

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 1 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pQCXIP-VEGFA121

Plasmid

#73017

Purpose

Retroviral expression of human VEGFA121

Depositor

Michael Grusch

Insert

VEGFA (VEGFA Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pTK168_mCherry-H2B

Plasmid

#46363

Purpose

visualizes chromosomes

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

H2B (H2BC11 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

D26G, V119I and S125D

Promoter

pBABE

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-HA-JOSD3

Plasmid

#22549

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

JOSD3 (TAF1D Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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betaRBPIG

Plasmid

#16422

Depositor

Juergen Hescheler

Article

Pfannkuche et al Nat Protoc. 2008. 3(9):1510-26. doi: htt

Insert

loxP flanked RFP and blasticidin-resistance plus puromycin-acetyltransferase IRES GFP

Expression

Mammalian

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pMKO shRNA Bim

Plasmid

#17235

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bim shRNA (BCL2L11 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pSicoR human Drosha2

Plasmid

#14767

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Drosha shRNA (DROSHA Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
cytoplasmic cpVenus control

Plasmid

#186788

Purpose

Identical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

Depositor

Michael Cohen

Article

Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

Insert

cytoplasmic cpVenus control

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

Plasmid

#171019

Purpose

Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

LargeBit-SNAPtag- Beta-actin

Use

Lentiviral and Luciferase

Tags

Beta actin and LargeBiT

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Mouse Cherry Brie Pooled Library

Pooled Library

#170511

Purpose

Genome-wide knockout library. It is identical to the Brie library #73633, replacing Puromycin resistance with mCherry.

Depositor

Pavel Tolar

Article

Newman et al bioRxiv 2021.03.02.433398

Expression

Mammalian

Species

Synthetic

Use

CRISPR and Lentiviral

Available Since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only
Mouse Metabolism Library

Pooled Library

#163966

Purpose

Knockout library targeting mouse metabolic genes.

Depositor

Alec Kimmelman

Article

Biancur et al Cell Metab. 2020 Oct 28. pii: S1550-4131

Use

CRISPR and Lentiviral

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only
pRosetta_v2

Plasmid

#136477

Purpose

Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-RAB10

Plasmid

#130883

Purpose

Expresses human RAB10 with N-terminal GFP tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

RAB10 (RAB10 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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