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pDisplay-NE2h-IRES-mCherry-CAAX

Plasmid

#208691

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h and a membrane-localized mcherry in mammalian cells

Depositor

Yulong Li

Article

Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2h

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD miniSOG

Plasmid

#91774

Purpose

Bacterial expression of a fluorescent protein, mini Singlet Oxygen Generator (miniSOG)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

mini Singlet Oxygen Generator

Tags

Hexa-Histidine tag

Expression

Bacterial

Promoter

araBAD

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pmScarlet-HSP70

Plasmid

#163790

Purpose

Expresses mScarlet-Hsp70 in mammalian cells

Depositor

Vincent Timmerman

Article

Adriaenssens et al Sci Rep. 2020 May 29;10(1):8755. doi: 10

Insert

HSP70 (HSPA1A Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Oskar 139-240 (eLOTUS domain) (HK73)

Plasmid

#206461

Purpose

Oskar 139-240 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Oskar 139-240 (osk Fly)

Tags

PH mcherry

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_g5-HT3.0mut

Plasmid

#208718

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut

Use

AAV

Promoter

hSyn

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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Activin A (S2-2)

Plasmid

#184621

Purpose

for transfection into S2 cells and expression of Activin A protein

Depositor

Timothy Springer

Article

Le et al J Mol Biol. 2022 Mar 15;434(5):167439. d

Insert

INHBA (INHBA Human)

Tags

8x His - streptavidin binding peptide

Expression

Insect

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-NE2m-IRES-mCherry-CAAX

Plasmid

#208690

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cells

Depositor

Yulong Li

Article

Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2m

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBJ FLAG-hPar1

Plasmid

#53226

Purpose

mammalian expression of human Par1 with N-terminal FLAG tag

Depositor

Shaun Coughlin

Article

Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

Insert

Par1 (F2R Human)

Tags

FLAG and bovine prolactin signal sequence (MDSKGS…

Expression

Mammalian

Mutation

Deletion of aa1-39 (native signal peptide)

Promoter

SV40

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pXG289

Plasmid

#141283

Purpose

OE of DELE1

Depositor

Martin Kampmann

Article

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

DELE1 (DELE1 Human)

Tags

mClover

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-TRIM28

Plasmid

#45568

Depositor

Fanxiu Zhu

Article

Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

Insert

TRIM28 (TRIM28 Human)

Tags

EGFP

Expression

Mammalian

Mutation

G34S mutation in EGFP

Promoter

CMV

Available Since

May 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2-mCherry-Cenexin

Plasmid

#186435

Purpose

Expresses mCherry Cenexin in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al Mol Biol Cell. 2022 Aug 1;33(9):br14. do

Insert

Cenexin (ODF2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Full-length WT talin1

Plasmid

#182992

Purpose

express FL WT talin1 in mammalian cells

Depositor

Vesa Hytönen

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

Talin1 WT (Tln1 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-TRIM28

Plasmid

#45570

Depositor

Fanxiu Zhu

Article

Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

Insert

TRIM28 (TRIM28 Human)

Tags

GST

Expression

Bacterial

Promoter

Ptac

Available Since

May 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1(+)mGAT1-0-GFP

Plasmid

#41662

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Tags

GFP

Expression

Mammalian

Mutation

C-terminal hydrophobic isoleucine residue added a…

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_eCBmut-IRES-mCherry-CAAX

Plasmid

#164612

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in mammalian cells

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Expression

Mammalian

Mutation

S383T, F177A

Promoter

CMV

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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DsRed-Rab5 DN

Plasmid

#13051

Depositor

Richard Pagano

Article

Sharma et al J Biol Chem. 2003 Feb 28. 278(9):7564-72

Insert

RAB5A (Canis familiaris) (RAB5A Canis familiaris)

Tags

DsRed2 and HA

Expression

Mammalian

Mutation

asparagine 3 changed to serine (neutral); aspara…

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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PDE9A_pcDNA6.2/EmGFP-Bsd

Plasmid

#176945

Purpose

Mammalian expression vector encoding PDE9A and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

PDE9A (PDE9A Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pTAMAHISTEV_PfeA

Plasmid

#128943

Purpose

expresses PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75)

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-neo.ARRDC1-mCherry

Plasmid

#38319

Depositor

Quan Lu

Article

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

ARRDC1 (ARRDC1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-N-HA-Pcdh15_mini_V4

Plasmid

#199185

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

HA-mmPcdh15_mini_V4-CD2

Use

AAV

Tags

Human influenza hemagglutinin (HA)

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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