167,318 results

1. TLCV2-ApaLI-T2A-EGFP-Puro

   Plasmid

   #234948

   Purpose

   Doxycycline-inducible mitochondria-localized ApaLI expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   ApaLI restriction enzyme with N-terminal COX8 mitochondrial localization signal
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   tight TRE promoter

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCXWB-EBNA1

   Plasmid

   #37624

   Purpose

   Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Insert

   EBNA-1 (EBNA-1 human herpesvirus 4)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3.1-FLAG-SF3B1-WT

   Plasmid

   #82576

   Purpose

   Expresses a codon-optimized ORF of human SF3B1 (wild-type)

   Depositor

   Manoj Pillai

   Article

   Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

   Insert

   human SF3B1-WT (SF3B1 Human)
   

   Tags

   FLAG peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-U6-sgRNA-PGK-puromycin

   Plasmid

   #51133

   Purpose

   expresses sgRNA from the U6 promoter.

   Depositor

   Xingxu Huang

   Article

   Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-T7pol

   Plasmid

   #59926

   Purpose

   Expression vector for T7 RNA polymerase gene

   Depositor

   Ian Wickersham

   Insert

   T7 RNA polymerase
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRKH3-ermGFP

   Plasmid

   #27169

   Depositor

   Michela Lizier

   Article

   Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

   Insert

   erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
   

   Expression

   Bacterial

   Mutation

   presence of an additional 183-bp region at N term…

   Available Since

   Feb. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Syn-ChrimsonR-tdT (AAV9)

   Viral Prep

   #59171-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-Syn-ChrimsonR-tdT (#59171). In addition to the viral particles, you will also receive purified pAAV-Syn-ChrimsonR-tdT plasmid DNA. Syn-driven ChrimsonR-tdTomato expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Promoter

   Syn

   Tags

   tdTomato

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pML104

   Plasmid

   #67638

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   single guide RNA expression cassette
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   pSNR52 and pTDH3

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCXLE-EGFP

   Plasmid

   #27082

   Purpose

   Integration-free (episomal) expression of EGFP

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Nat Methods. 2011 May;8(5):409-12

   Insert

   eGFP
   

   Expression

   Mammalian

   Available Since

   March 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV1)

    Viral Prep

    #162378-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (#162378). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8m-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    Syn

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only