We narrowed to 3,645 results for: MAK;

1. pcDNA4/TO/Strep-HA-ZAKalpha_deltaS

   Plasmid

   #141197

   Purpose

   Expresses Strep-HA-ZAKalpha_deltaS in mammalian cells, can be used to make inducible cell lines

   Depositor

   Simon Bekker-Jensen

   Article

   Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

   Insert

   MAP3K20 (MAP3K20 Human)
   

   Use

   Tags

   Strep, HA

   Expression

   Mammalian

   Mutation

   deletion of amino acids 670-712

   Promoter

   CMV

   Available since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLV-shFMR1-mCherry

   Plasmid

   #222963

   Purpose

   Make lentivirus to knock down human FMR1 gene

   Depositor

   Xinyu Zhao

   Article

   Guo et al Nat Commun. 2023 Jun 26;14(1):3801. doi:

   Insert

   FMR1 shRNA (FMR1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLX304-BCL-XL-499/500R

   Plasmid

   #203575

   Purpose

   Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499, TRCN0000033500) in mammalian cells.

   Depositor

   Abhishek Chakraborty

   Article

   Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   V5-tagged

   Expression

   Mammalian

   Mutation

   silent point mutations to make resistant shRNA 49…

   Promoter

   CMV

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pD649-HAsp-SEMA7A-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156922

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA7A (SEMA7A Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mTet1 flox donor

   Plasmid

   #117147

   Purpose

   Making Tet1 conditional knock out

   Depositor

   Jacob Hanna

   Article

   Zviran et al ssrn.3155731

   Insert

   Tet1 (Tet1 Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CCSB-Broad Human Kinase ORF Collection

   Plasmid Kit

   #1000000014

   Purpose

   556 distinct human kinases and kinase-related protein ORFs in a pDONR-223 Entry Gateway vector. All clones are clonal isolates.

   Depositor

   David Root, William Hahn

   Available since

   July 16, 2011

   Availability

   Academic Institutions and Nonprofits only

7. A3Bi-ctd E255A -Cas9n-UGI-NLS

   Plasmid

   #109429

   Purpose

   Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Insertion of an L1 intron into the coding sequenc…

   Promoter

   CMV

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-GST-ATG9 (828-839)-ATG101 (1-198)

   Plasmid

   #188073

   Purpose

   To make ATG101/ATG13-HORMA and ATG9 C-terminal tail fusion complex for expression in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 and ATG101 (ATG9A, ATG101 Human)
   

   Use

   Tags

   GST tag

   Expression

   Mammalian

   Mutation

   codon-optimized ATG9 (828-839) and codon-optimize…

   Promoter

   CMV

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHD-dsRed-attP_dCrk_flankingHRs

   Plasmid

   #131141

   Purpose

   Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Inserts

   Crk Upstream Homology Region (Crk Fly)
   Crk Downstream Homology Region (Crk Fly)
   

   Use

   CRISPR and Cre/Lox; Homology directed repair temp…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV-hUbC-EGFP-sgRNA.FOXA1/A2/A3_CRISPRi

    Plasmid

    #216166

    Purpose

    Express the gRNAs for the human FOXA1/A2/A3-CRISPRi

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA-FOXA1-Rank1, sgRNA-FOXA2-Rank2, sgRNA-FOXA2-Rank1, and sgRNA-FOXA3-Rank1 (FOXA1, FOXA2, FOXA3 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    phU6, ph7SK, phH1, pmU6

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Strain KJ1C

    Bacterial Strain

    #21874

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Protoc. 2009. 4(10):1471-501.

    Bacterial Resistance

    Tetracycline

    Available since

    Nov. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

12. Strain CSH100

    Bacterial Strain

    #21875

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Protoc. 2009. 4(10):1471-501.

    Bacterial Resistance

    None

    Available since

    Nov. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

13. HL 3325 (=HL 770 + rhyB knockout_KanR)

    Bacterial Strain

    #30065

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Bacterial Resistance

    Kanamycin

    Species

    Escherichia coli

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

14. BL21 ΔrecBCD

    Bacterial Strain

    #176581

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.

    Depositor

    Jean-Loup Faulon, Chase Beisel

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    None

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

15. BL21 Rosetta2 ΔrecBCD

    Bacterial Strain

    #176583

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

16. BL21 ΔrecB

    Bacterial Strain

    #176580

    Purpose

    E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression.

    Depositor

    Jean-Loup Faulon, Chase Beisel

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    None

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

17. BL21 Rosetta2 ΔrecB

    Bacterial Strain

    #176582

    Purpose

    E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

18. BL21 Rosetta2 ΔrecBCD GamS

    Bacterial Strain

    #176586

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol and ampicillin

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

19. BL21 Rosetta2 GamS

    Bacterial Strain

    #176584

    Purpose

    E. coli BL21 strain for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol and ampicillin

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

20. BL21 Rosetta2 ΔrecB GamS

    Bacterial Strain

    #176585

    Purpose

    E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol and ampicillin

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only