We narrowed to 6,993 results for: Cad

1. pAc5.1B-lambdaN-HA-DmAGO1-Y653L-siRNAres_J

   Plasmid

   #146716

   Purpose

   Insect Expression of DmAGO1-Y653L-siRNAres

   Depositor

   Elisa Izaurralde

   Article

   Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

   Insert

   DmAGO1-Y653L-siRNAres (AGO1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   variation with 2 non silent mutations compared to…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuetG-HsDCP1a_539-582 (MCSII)_G

   Plasmid

   #146407

   Purpose

   Bacterial Expression of HsDCP1a_539-582

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   HsDCP1a_539-582 (DCP1A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   one silent mutation T1716G compared to the sequen…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut2_G

   Plasmid

   #146409

   Purpose

   Bacterial Expression of HsDCP1a_539-582-mut2

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   HsDCP1a_539-582-mut2 (DCP1A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   one silent mutation T1716G compared to the sequen…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRSFDuetG-HsDCP1a_539-582 (MCSII)-WT_G

   Plasmid

   #146411

   Purpose

   Bacterial Expression of HsDCP1a_539-582

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   HsDCP1a_539-582 (DCP1A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   one silent mutation T1716G compared to the sequen…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-Dest-MBP-Nsp1

   Plasmid

   #191483

   Purpose

   Mammalian expression of MBP-tagged SARS-CoV-2 Nsp1

   Depositor

   Hao Wu

   Article

   Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

   Insert

   MBP-Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAFMW-Nbr[R272A,K276A, R280A,K284A]

   Plasmid

   #188599

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Use

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   R272A [CGG=> GCG], K276A [AAG=>GCG], R280A …

   Promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS2-hFAM3B-HA

   Plasmid

   #180145

   Purpose

   Coding sequence of human FAM3B pCS2 with C terminal HA tag

   Depositor

   Edward De Robertis

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

   Insert

   FAM3B (FAM3B Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPOLY_new

   Plasmid

   #179276

   Purpose

   Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

   Inserts

   cellodextrin phosphorylase
   cellobiose phosphorylase
   sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
   

   Use

   Synthetic Biology

   Tags

   6xHIS and Strep-Tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lacO

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBICI_high ori

   Plasmid

   #179273

   Purpose

   Expresses CuCBP and BaSP in E. coli BL21(DE3)

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lacO

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDUBI

    Plasmid

    #179274

    Purpose

    Expresses CcCDP monocistronically and CuCBP and BaSP bicistronically

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Inserts

    cellodextrin phosphorylase
    cellobiose phosphorylase
    C8077_RS00435 sucrose phosphorylase [ Bifidobacterium adolescentis (C8077_RS00435 Bifidobacterium alodescentis)
    

    Use

    Synthetic Biology

    Tags

    6xHIS and Strep-Tag II

    Expression

    Bacterial

    Mutation

    sucrose phosphorylase

    Promoter

    T7 lacO

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2-hFAM3B-FLAG

    Plasmid

    #180146

    Purpose

    Coding sequence of human FAM3B in pCS2 with C terminal FLAG tag

    Depositor

    Edward De Robertis

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

    Insert

    FAM3B (FAM3B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCSJ982

    Plasmid

    #176622

    Purpose

    Express yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

    Depositor

    Alexander Varshavsky

    Article

    Dong et al Proc Natl Acad Sci U S A. 2020 Jun 23;11

    Insert

    Ub-MPGLW-Fbp1
    

    Use

    Tags

    Ubiquitin N-terminal fusion and triple flag tag C…

    Expression

    Yeast

    Mutation

    Changed first 4 N-terminal amino acid residues of…

    Promoter

    TDH3tc3

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAc5.1B-lambdaN-HA-DmAGO1-K690A_M

    Plasmid

    #146957

    Purpose

    Insect Expression of DmAGO1-K690A

    Depositor

    Elisa Izaurralde

    Article

    Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

    Insert

    DmAGO1-K690A (AGO1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    variation with 2 non silent mutations V315A and H…

    Promoter

    Available since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mEos2-dVHH[GFP] in pCSDEST

    Plasmid

    #162870

    Purpose

    Super-resolution of GFP in culture

    Depositor

    Rob Parton

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    mEos2-dVHH[EGFP]
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mILK-R349A-EFGP

    Plasmid

    #176898

    Purpose

    mouse ILK with R349A point mutation cloned into pEGFP-N1

    Depositor

    Sara Wickström

    Article

    Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    Integrin-linked kinase (Ilk Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Arginine 349 to Alanine

    Promoter

    CNV

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mILK-R255A-EFGP

    Plasmid

    #176897

    Purpose

    mouse ILK with R255A point mutation cloned into pEGFP-N1

    Depositor

    Sara Wickström

    Article

    Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    Integrin-linked kinase (Ilk Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Arginine 255 to Alanine

    Promoter

    CNV

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPodA11

    Plasmid

    #162843

    Purpose

    Expression protein designed PodA10 from a TEV cleavable IPTG inducible plasmid with ampicillin resistance

    Depositor

    Dianne Newman

    Article

    VanDrisse et al Proc Natl Acad Sci U S A. 2021 Mar 23;11

    Insert

    Protein Designed Pyocyanin demethylase
    

    Use

    Tags

    TEV-cleavable 6x-His tag

    Expression

    Bacterial

    Mutation

    A53N, I73T, A87V, M99V, A129T from WT PodA

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28_6xHis-PARP1delZn1-L713F

    Plasmid

    #173937

    Purpose

    Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn1-L713F (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 1-96; L713F, V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28_6xHis-PARP1delZn3-L713F

    Plasmid

    #173934

    Purpose

    Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn3-L713F (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 214-373; L713F, V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28_6xHis-PARP1-G871L

    Plasmid

    #174798

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-G871L (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    G871L, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28_6xHis-PARP1-A870L

    Plasmid

    #174797

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-A870L (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A870L, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28_6xHis-PARP1-K893I

    Plasmid

    #174799

    Purpose

    Bacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-K893I (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K893I, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET41sIII_GST-ALC1-K77R

    Plasmid

    #174813

    Purpose

    Bacterial expression of inactive ALC1 (K77R disrupts ATP binding)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    ALC1-K77R (CHD1L Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    K77R

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET41sIII_GST-ALC1-E175Q

    Plasmid

    #174814

    Purpose

    Bacterial expression of inactive ALC1 (E175Q disrupts ATP binding)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    ALC1-E175Q (CHD1L Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E175Q

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. deltaLoop-MIDAS

    Plasmid

    #171605

    Purpose

    Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) without the loop region (a.a. 4458-4496) in bacteria.

    Depositor

    Tarun Kapoor

    Article

    Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

    Insert

    MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
    

    Use

    Tags

    His6 tag and SNAP tag

    Expression

    Bacterial

    Mutation

    Replaced amino acids 4458-4496 with ASGSGS linker

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. KIF13B

    Plasmid

    #163907

    Purpose

    Full length mouse KIF13B for purification from insect cells

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    kif13b (Kif13b Mouse)
    

    Use

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. KIF13B 1-584

    Plasmid

    #163908

    Purpose

    Motile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    kif13b (Kif13b Mouse)
    

    Use

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDNA3/flag-pBirA(1-K283)-FRB

    Plasmid

    #154451

    Purpose

    expresses flag-pBirA(1-K283)-FRB/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    flag-pBirA(1-K283)-FRB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCEP4-myc-H2-Dd (Y84C/C121S/A139C)

    Plasmid

    #136063

    Purpose

    Mammalian expression of myc-tagged H2-Dd (Y84C/C121S/A139C mutant)

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    H2-Dd (H2-D1 Mouse)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Y84C/C121S/A139C

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCEP4-myc-HLA-B15-VN

    Plasmid

    #135514

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-B*15:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*15:01 (HLA-B Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge