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pcDNA4/HisMax-Synectin

Plasmid

#35943

Depositor

Arie Horowitz

Article

Naccache et al Proc Natl Acad Sci U S A. 2006 Aug 22;10

Insert

Synectin (Gipc1 Mouse)

Tags

6xHIS and Xpress

Expression

Mammalian

Mutation

K111R

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pUHD.NFATc1nuc

Plasmid

#40616

Depositor

Jerry Crabtree

Article

Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

Insert

NFATc1nuc (NFATC1 Human)

Tags

HA

Expression

Mammalian

Mutation

S172A S175A S176A S178A S179A S181A S184A S187A S…

Promoter

CMV2

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-hs-ALPS 1-ALPS2-EGFP

Plasmid

#187113

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000101199 (ARFGAP1 Human)

Tags

EGFP

Expression

Bacterial

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-FoxG1

Plasmid

#35547

Depositor

Marius Wernig

Article

Lujan et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

Insert

FoxG1 (Foxg1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only

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pEF-ABEmax

Plasmid

#164415

Purpose

EF1alpha promoter + ABEmax (derived from #112095). Mammalian cell adenosine base editor expression vector.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

ABEmax

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pET19-MTdTwt

Plasmid

#207460

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

MTdTwt (Dntt Mouse)

Tags

10xHis-MBP

Expression

Bacterial

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-D2s-L-Venus

Plasmid

#19966

Depositor

Jonathan Javitch

Article

Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

Insert

D2 short variant-monomeric Venus (DRD2 Human, Aequorea Victoria)

Tags

Flag and monomeric Venus

Expression

Mammalian

Available Since

Jan. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3A WT

Plasmid

#123069

Purpose

Gateway entry clone encoding human MAP1LC3A wild-type

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Use

Gateway entry vector / entry clone

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pEF-ManII-GFP

Plasmid

#160905

Purpose

Expresses the Golgi marker ManII-GFP in mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

MAN2A1 (MAN2A1 Human)

Tags

GFP

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP.N1-HDAC6.delta Buz

Plasmid

#36190

Depositor

Tso-Pang Yao

Article

Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-2

Insert

HDAC6..delta Buz (HDAC6 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the C-terminal ub binding domain (BUZ…

Promoter

CMV

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pBabe Angptl4 puro

Plasmid

#19156

Depositor

Joan Massague

Article

Padua et al Cell. 2008 Apr 4. 133(1):66-77.

Insert

angiopoietin-like 4 (ANGPTL4 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv1 sgTollip

Plasmid

#196546

Purpose

Lentiviral CRISPR-Cas9 plasmid containing gRNA targeting exon 1 of human Tollip. Used for generation of Tollip protein knockouts in human cell lines.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLENTI-CMV-GFP-TREX1 delta C (aa1-235) BLAST

Plasmid

#164229

Purpose

lentiviral plasmid for GFP-hTREX1 delta C (aa1-235)

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 235-314

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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lentiviral shRNA GEF-H1

Plasmid

#21477

Depositor

Richard Huganir

Article

Kang et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

Insert

rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2 Rat)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pLENTI CMV GFP-TREX1 delta N (aa236-314) BLAST

Plasmid

#164235

Purpose

lentiviral plasmid for GFP-TREX1 delta N (aa236-314)

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deletion of amino acids 1-234

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pEW3: pET-32a Dot1L(2-416)

Plasmid

#124098

Purpose

Dot1L(2-416) expression plasmid

Depositor

Cynthia Wolberger

Article

Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

Insert

Dot1L(2-416) (DOT1L Human)

Tags

Trx-His-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pQlinkH-PTPIP51(36-235)

Plasmid

#170533

Purpose

Expresses Cleavable His-tagged PTPIP51_ΔTPR domain (36-235) in E. Coli

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)

Tags

His tag

Expression

Bacterial

Promoter

T5 promoter

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCR4-huNFATc1nuc

Plasmid

#23988

Depositor

Jerry Crabtree

Article

Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

Insert

NFATc1 (NFATC1 Human)

Use

Cloning vector

Tags

EE tag

Mutation

S172A S175A S176A S178A S179A S181A S184A S187A S…

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pSL0812 (pCRISPR(BbsI)_Vch)

Plasmid

#200898

Purpose

Expresses V. cholerae CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCRISPR (entry)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

Plasmid

#200910

Purpose

3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

Depositor

Debojyoti Chakraborty

Insert

3XHA_den1FnCas9_KRAB_eGFP

Use

CRISPR

Tags

3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP

Expression

Mammalian

Mutation

Changed Glutamic Acid to Arginine at 1369 position

Promoter

CBH

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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