We narrowed to 4,862 results for: Mela

1. VP-ARF

   Plasmid

   #89122

   Purpose

   mammalian expression of p14-hARF linked to VP16 activation domain

   Depositor

   Elizabeth Wilson

   Article

   Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87

   Insert

   p14-hARF (CDKN2A Human)
   

   Use

   Tags

   VP16

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

   Plasmid

   #113039

   Purpose

   AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCrysβ:ECFP-LexOP:mCherry-HrasG12V

   Plasmid

   #89887

   Purpose

   Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   mCherry-HRasG12V
   

   Use

   Unspecified

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-Y1214F-VEGFR2-mCherry

   Plasmid

   #139609

   Purpose

   Expresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cells

   Depositor

   Michael Welsh

   Article

   Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

   Insert

   KDR (KDR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSUPER-retro-puro-shOrai1

   Plasmid

   #89933

   Purpose

   Knockdown human Orai1 expression

   Depositor

   Shengyu Yang

   Article

   Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

   Insert

   Orai1 shRNA (ORAI1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCrysβ:ECFP-LexOP:mCherry-NRasQ61K

   Plasmid

   #89886

   Purpose

   Human NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   mCherry-NRasQ61K
   

   Use

   Unspecified

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUAST-attB-3xHA-V5-RpL10Ab

   Plasmid

   #125223

   Purpose

   3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

   Depositor

   Herman Dierick

   Article

   Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

   Insert

   RpL10Ab (RpL10Ab Fly)
   

   Use

   Tags

   3x HA, V5

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc-y1sgRNA-Cas9

   Plasmid

   #49331

   Purpose

   Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   Ji-Long Liu

   Article

   Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

   Inserts

   Cas9
   dU6-sgRNA
   y1 sgRNA (y Fly)
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Insect

   Mutation

   Human codon optimised

   Promoter

   Actin-5c and Drosophila U6

   Available Since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDropIn-T2A-split-intein-Gal4[21-881]-C-int

   Plasmid

   #199196

   Purpose

   Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLNCX2-STIM1

    Plasmid

    #89817

    Purpose

    Express HUMAN STIM1

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    NO TAG

    Expression

    Mutation

    Promoter

    Available Since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSUPER-retro-puro-shSTIM1

    Plasmid

    #89816

    Purpose

    Knockdown human STIM1 expression

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    STIM1 shRNA (STIM1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL3-DICER-Prom

    Plasmid

    #25851

    Depositor

    David E. Fisher

    Article

    Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

    Insert

    Dicer promoter (DICER1 gene ID 23405, Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLVX-Phi3-Zsgreen-PGK-mCherry

    Plasmid

    #153320

    Purpose

    Expresses zsGreen and mCherry in mammalian cells.

    Depositor

    Robert Judson-Torres

    Article

    McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

    Insert

    zsGreen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KHBD00476

    Plasmid

    #39585

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG5838 (Dref Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

15. UAS-dsarm(E)

    Plasmid

    #187870

    Purpose

    Gal4/UAS expression of dSarm(isoE)

    Depositor

    Lukas Neukomm

    Article

    Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

    Insert

    dSarm isoform E (Sarm Fly)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GAL-FKBP51

    Plasmid

    #89132

    Purpose

    mammalian expression of FKBP5 also referred to as FKBP51 with N-terminal GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

    Insert

    FKBP5 (FKBP5 Human)
    

    Use

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-lambdaN-HA-DmeIF4G-V5His6

    Plasmid

    #79259

    Purpose

    Expresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cells

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

    Insert

    DmeIF4G (eIF4G1 Fly)
    

    Use

    Schneider 2 cell expression

    Tags

    V5-His6 and lambdaN-HA

    Expression

    Mutation

    Promoter

    Ac5 promoter

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. prk5-MFSD12-TEV-3xFLAG-3xHA

    Plasmid

    #149504

    Purpose

    Transient expression of human MFSD12 with recoded nucleotide sequence.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    MFSD12 (MFSD12 Human)
    

    Use

    Transient expression

    Tags

    TEV-3XHA-3XFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized Sequence

    Promoter

    CMV

    Available Since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Hy_pMT Laccase2 Exons 1-3

    Plasmid

    #91799

    Purpose

    Drosophila laccase2 minigene plasmid that expresses both a linear RNA and a circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Mol Cell. 2017 Nov 9. pii: S1097-2765(17

    Insert

    Laccase2 (stw Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc-GFP-dSUMO

    Plasmid

    #85696

    Purpose

    expression of GFP-SUMO1 in Drosophila cells

    Depositor

    Giannino Del Sal, Licio Collavin

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    SUMO (Sumo Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Ac5

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only