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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 29,147 results for: Tat

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Showing: 3541 - 3560 of 29147 results

  4. eft-3p:act-5(ptc):tbb2 3' UTR

    Plasmid
    #202331
    Purpose
    The ubiquitous eft-3 promoter drives overexpression of the mutant act-5(ptc) gene including introns.
    Depositor
    Didier Stainier
    Article
    Welker et al PLoS Genet. 2023 Jun 29;19(6):e1010806.
    Inserts
    eft-3 promoter
    act-5(ptc)
    tbb-2 3' UTR
    Tags
    base 238 C -> A mutation causing a PTC at codo…
    Expression
    Worm
    Promoter
    This is a promoter sequence for C. elegans eft-3,…
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202331-plasmid-map-sequence-id-396314

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  5. pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

    Plasmid
    #191572
    Purpose
    Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    SARS-CoV-2 Spike Protein (S Synthetic)
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    D614G and F486S (confers reduced binding affinity…
    Promoter
    CMV
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191572-plasmid-map-sequence-id-441975

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  6. AA315

    Plasmid
    #215951
    Purpose
    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control lethal
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    rev{U6_v1; DR_v0 [EnAs]; sgPLK1_v1 [EnAs]; DR_v1 [EnAs]; sgPLK1_v2 [EnAs]}
    Use
    CRISPR; Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215951-plasmid-map-sequence-id-431482

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  9. EGFP-MYO10-FERM-ITGBD

    Plasmid
    #194855
    Purpose
    Express EGFP-MYO10 with mutations (S2001_F2002insA/T2009D) that interfere with ITGB binding in mammalian cells.
    Depositor
    Guillaume Jacquemet
    Article
    Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi
    Insert
    MYO10 S2001_F2002insA/T2009D (MYO10 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    MYO10 has the following mutations S2001_F2002insA…
    Promoter
    CMV
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    194855-plasmid-map-sequence-id-389000

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  13. pLCHKO_SFRS7_exon_deletion_2

    Plasmid
    #155070
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155070-plasmid-map-sequence-id-308588

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  14. pLCHKO_MDM4_exon_deletion_2

    Plasmid
    #155074
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155074-plasmid-map-sequence-id-308585

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  15. pLCHKO_Ptbp_ex8_2

    Plasmid
    #155082
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155082-plasmid-map-sequence-id-308510

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  17. pMCh-660

    Plasmid
    #92259
    Purpose
    Plasmid for expression of NHA-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cells
    Depositor
    Marina Chekulaeva, Witold Filipowicz
    Article
    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1
    Insert
    TNRC6C CED (TNRC6C Human)
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    C-terminal effector domain (CED): aa 1369-1690 wi…
    Promoter
    CMV
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    92259-plasmid-map-sequence-id-194509

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  18. pMCh-665

    Plasmid
    #92254
    Purpose
    Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cells
    Depositor
    Marina Chekulaeva, Witold Filipowicz
    Article
    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1
    Insert
    TNRC6C CED (TNRC6C Human)
    Tags
    GST
    Expression
    Mammalian
    Mutation
    C-terminal effector domain (CED): aa 1369-1690 wi…
    Promoter
    EF1A
    Available Since
    Oct. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    92254-plasmid-map-sequence-id-194411

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Showing: 3541 - 3560 of 29147 results