We narrowed to 16,784 results for: crispr grnas

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pWT062e

Plasmid

#107910

Purpose

W2m.2-3 plasmid. Contains U6-LacI-sgRNA A and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

U6-LacI-sgRNA A

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pWT062e

Plasmid

#107910

Purpose

W2m.2-3 plasmid. Contains U6-LacI-sgRNA A and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

U6-LacI-sgRNA A

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pWT016l

Plasmid

#96857

Purpose

(d)theophylline-agRNA-GFP

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)theophylline-agRNA-GFP

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT016l

Plasmid

#96857

Purpose

(d)theophylline-agRNA-GFP

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)theophylline-agRNA-GFP

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-NEAT1m_v1

Plasmid

#97083

Purpose

Encodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoform

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1m_v1

Use

CRISPR

Promoter

U6

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-NEAT1m_v1

Plasmid

#97083

Purpose

Encodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoform

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1m_v1

Use

CRISPR

Promoter

U6

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMM710

Plasmid

#68893

Purpose

IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pMM710

Plasmid

#68893

Purpose

IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCRISPR-mcBEST

Plasmid

#209416

Purpose

Plasmid for multiplexed cytosine base editing in streptomycetes

Depositor

Tilmann Weber

Article

Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2

Inserts

Codon optimized APOBEC1-nCas9-UGI fusion protein
csy4 from Pseudomonas aeruginosa PAO1

Use

CRISPR and Synthetic Biology

Promoter

base editing cassette: PtipA ; sgRNAs: PkasO* an…

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR-mcBEST

Plasmid

#209416

Purpose

Plasmid for multiplexed cytosine base editing in streptomycetes

Depositor

Tilmann Weber

Article

Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2

Inserts

Codon optimized APOBEC1-nCas9-UGI fusion protein
csy4 from Pseudomonas aeruginosa PAO1

Use

CRISPR and Synthetic Biology

Promoter

base editing cassette: PtipA ; sgRNAs: PkasO* an…

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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B270 (plasmid expressing ATP1A1 sgSTOP and containing an empty sgRNA-expression cassette)

Plasmid

#100716

Purpose

B52 plasmid expressing ATP1A1 sgSTOP (cloned in BsmBI site) and containing an empty sgRNA-expression cassette (use BbsI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting ATP1A1 (cloned using BsmBI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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B270 (plasmid expressing ATP1A1 sgSTOP and containing an empty sgRNA-expression cassette)

Plasmid

#100716

Purpose

B52 plasmid expressing ATP1A1 sgSTOP (cloned in BsmBI site) and containing an empty sgRNA-expression cassette (use BbsI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting ATP1A1 (cloned using BsmBI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_TIA1

Plasmid

#106097

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIA1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA TIA1

Use

CRISPR

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_TIA1

Plasmid

#106097

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIA1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA TIA1

Use

CRISPR

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_ELAVL1

Plasmid

#106106

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting ELAVL1 (ELAVL1 Human)

Use

CRISPR

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_ELAVL1

Plasmid

#106106

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting ELAVL1 (ELAVL1 Human)

Use

CRISPR

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pgRNA-non-target

Plasmid

#129465

Purpose

Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Non-target/random gRNA

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pgRNA-non-target

Plasmid

#129465

Purpose

Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Non-target/random gRNA

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSTBlue-U6-sgRNA(tdTomato)

Plasmid

#47871

Purpose

This plasmid contains expression cassette for sgRNA targeting tdTomato.

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Insert

sgRNA-tdTomato

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pSTBlue-U6-sgRNA(tdTomato)

Plasmid

#47871

Purpose

This plasmid contains expression cassette for sgRNA targeting tdTomato.

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Insert

sgRNA-tdTomato

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge