We narrowed to 12,905 results for: NUC
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Plasmid#102560PurposePiggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonExpressionMammalianPromoterU6Available SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only
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pEGFP NFAT5 (no EGFP)
Plasmid#13627DepositorAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
FR_luc
Plasmid#31098DepositorInsertfirefly luciferase
UseFlp/frtExpressionMammalianAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
LiRTi
Plasmid#214076PurposeLuminescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTRDepositorInsertFirefly luciferase
ExpressionMammalianMutationLinker sequence present between firefly luciferas…Available SinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD_Kan-LacI
Plasmid#79826PurposeArabinose induced expression of Lac repressorDepositorInsertLacI
TagsHis6ExpressionBacterialPromoteraraBpAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBTK622
Plasmid#180592Purposetdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1DepositorInsertstdk
kanR
UseSynthetic BiologyAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sp_PE
Plasmid#212038PurposeBacterial expression of the PE2 proteins for genome editing in E. coliDepositorInsertPE2
UseCRISPRPromoterL-arabinose-inducible promoter PBADAvailable SinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL685
Plasmid#205592PurposeNEPE for prime editing in riceDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionPlantPromoterZmUbiAvailable SinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEVA63_HelperΔgIII
Plasmid#190433PurposeM13 helper genes cloned into the pSEVA631 vector, except the minor coat protein coding gene gIII (also called gp3, or g3p). This plasmid does not carry an M13 packaging signal.DepositorInsertM13 genes I-V and VII-XI
UseSynthetic BiologyAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCold CL7-Cas9
Plasmid#124890Purposeobtaining full Cas9 RNPDepositorInsertCas9
ExpressionBacterialAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEVA63_Helper
Plasmid#190432PurposeM13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ297.)DepositorInsertM13 genes: I-XI
UseSynthetic BiologyAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCh-Cry2olig
Plasmid#101222PurposeCry2olig(E490G)DepositorInsertCry2olig
UseLentiviralExpressionMammalianMutationE490GPromoterSFFVAvailable SinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
coel::RFP
Plasmid#8938DepositorAvailable SinceNov. 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
4xNLS-pMJ915v2-sfGFP
Plasmid#88921PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRExpressionBacterialAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AncMEcoGII250
Plasmid#205025PurposeExpressing engineered M.EcoGII with higher thermostabilityDepositorInsertAncestral M.EcoGII 250
TagsHis6x tagExpressionBacterialPromoterT7Available SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-SF2
Plasmid#17990DepositorAvailable SinceMay 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pWJ66
Plasmid#46570PurposeSame as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)DepositorInsertstracrRNA
dcas9-w
CRISPR array
UseCRISPRTagsrpoZExpressionBacterialMutationD10A, H840AAvailable SinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AsCas12f1
Plasmid#171614PurposeAsCas12f1-based Human cell genome editingDepositorInsertAsCas12f1 and sgRNA_v1
ExpressionMammalianAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE3G-dCas9-EGFP-HaloTag
Plasmid#169451Purposeexpression of dCas9-EGFP-HaloTagDepositorInsertdCas9-EGFP-HaloTag
UseLentiviralAvailable SinceJune 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP(C1)-PP1alpha
Plasmid#44224DepositorAvailable SinceDec. 4, 2013AvailabilityAcademic Institutions and Nonprofits only