We narrowed to 24,999 results for: Nes

1. pCMV-MMLVgag-3xNES-ABE8e-NG

   Plasmid

   #181754

   Purpose

   MMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLVgag-3xNES-ABE8e-NG
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-MMLVgag-3xNES-Cas9

   Plasmid

   #181752

   Purpose

   MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLV-Gag-3xNES-Cas9 (Spy)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-GFP-TurboID-NES_pLX304

   Plasmid

   #202019

   Purpose

   expresses GFP-TurboID in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   Flag-GFP-TurboID-NES
   

   Use

   Lentiviral

   Tags

   Flag and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-RNaseHI-NES-EGFP

   Plasmid

   #196701

   Purpose

   Plasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

   Insert

   bacterial RNaseHI
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-CMV-NES-jRCaMP1a

   Plasmid

   #61562

   Purpose

   Red fluorescent calcium sensor protein

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Insert

   NES-jRCaMP1a
   

   Use

   Tags

   nuclear export signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-dCas13-inactive M3M14nes

   Plasmid

   #157855

   Purpose

   Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A; METTL3 D395A

   Promoter

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-MMLVgag-3xNES-ABE7.10-NG

   Plasmid

   #181753

   Purpose

   MMLV-Gag fused to 3xNES-ABE7.10-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLVgag-3xNES-ABE7.10-NG
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-CMV-NES-jRCaMP1b

   Plasmid

   #63136

   Purpose

   Red fluorescent calcium sensor protein

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Insert

   NES-jRCaMP1b
   

   Use

   Tags

   nuclear export signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_hSyn_NES-Kinprola_PKA-mEGFP_WRPE-SV40

   Plasmid

   #233366

   Purpose

   hSyn1 driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for neuronal expression through AAV transduction

   Depositor

   Kai Johnsson

   Article

   Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

   Insert

   NES-Kinprola_PKA-mEGFP
   

   Use

   AAV

   Tags

   NES and mEGFP

   Expression

   Mutation

   Promoter

   hSyn1

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGEN-dPspCas13b-NES-mCherry

    Plasmid

    #233609

    Purpose

    Expression of catalytically-inactive PspCas13b in mammalian cells

    Depositor

    Shintaro Iwasaki

    Article

    Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

    Insert

    dPspCas13b-NES-mCherry
    

    Use

    CRISPR

    Tags

    3xFlag and mCherry

    Expression

    Mutation

    H133A/H1058A

    Promoter

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

    Plasmid

    #194681

    Purpose

    CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_01-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA5/FRT_CMV_NES-Caprola_04-mEGFP

    Plasmid

    #194682

    Purpose

    CMV driven expression of the calcium recorder Caprola_04 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_04-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX304-Flag-APEX2-NES

    Plasmid

    #92158

    Purpose

    Cytosolic APEX2 construct

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    Flag-APEX2-NES
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAB1678 CMV-HIVNES-GS-Cas13bt1

    Plasmid

    #176316

    Purpose

    CMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    human codon optimized Cas13bt1
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG1243 NES-ECFP-FIREmate

    Plasmid

    #205169

    Purpose

    Expresses NES-ECFP-FIREmate in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    NES-ECFP-FIREmate
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid

    #182287

    Purpose

    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    

    Use

    Tags

    nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Y306A/Y384F (AF) double mutant of Methanosarcina …

    Promoter

    CMV and U6

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCSC-GFP-NES-EGFP

    Plasmid

    #221810

    Purpose

    Lentiviral vector expressing green fluorescent protein (GFP) fused with nuclear export sequence (NES).

    Depositor

    Baojin Ding

    Article

    Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

    Insert

    GFP-NES-EGFP
    

    Use

    Lentiviral

    Tags

    NES (internal tag)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. NES-EGFP-PH-ARNO2G-I303Ex2

    Plasmid

    #116868

    Purpose

    PIP3 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    CYTH2 (CYTH2 Human)
    

    Use

    Tags

    EGFP and X.leavis map2k1.L(32-44)

    Expression

    Mammalian

    Mutation

    amind acids 252-399, isoleucine 303 changed to gl…

    Promoter

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-Camk2-NES-Twitch-NR

    Plasmid

    #173043

    Purpose

    TnC-based calcium sensor with green-red FRET pair ratiometric readout

    Depositor

    Yiyang Gong

    Article

    Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

    Insert

    NES-Twitch-NR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamK2

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. R4pGWB601_UBQ10p-Turbo-NES-YFP

    Plasmid

    #127366

    Purpose

    Binary vector for expressing cytosolic TurboID-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Plant

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Arabidopsis UBQ10 promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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