We narrowed to 399 results for: Tight TRE

1. pCW57-mCherry-2A-BRD4 Iso CdelET

   Plasmid

   #137723

   Purpose

   Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)

   Depositor

   Scott Floyd

   Article

   Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

   Insert

   BRD4 short isoform with deleted extra-terminal domain (BRD4 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deleted amino acids 600-682

   Promoter

   Tight TRE promoter

   Available Since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PA-mCherry-miniSOG-VE-Cadherin-N-10

   Plasmid

   #57799

   Purpose

   Localization: Tight Junctions, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   VE-Cadherin (CDH5 Human)
   

   Use

   Tags

   PA-mCherry-miniSOG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

3. AAVS1-iPE-Plus

   Plasmid

   #214062

   Purpose

   inducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locus

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   PE-Plus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-tight

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FUT8-Dox

   Plasmid

   #124631

   Purpose

   Doxycycline-inducible FUT8 expression in mammalian cells

   Depositor

   Ron Weiss

   Article

   Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

   Inserts

   Alpha-(1,6)-fucosyltransferase
   Tetracycline repressor A3 mutant
   TagFP635
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-tight and hEF1a

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. B4GALT1-Dox

   Plasmid

   #124633

   Purpose

   Doxycycline-inducible B4GALT1 expression in mammalian cells

   Depositor

   Ron Weiss

   Article

   Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

   Inserts

   Beta-(1,4)-Galactosyltransferase 1
   Tetracycline repressor A3 mutant
   TagFP635
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-tight and hEF1a

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCW57-Cx26-IRES-GCaMP6s

   Plasmid

   #188236

   Purpose

   3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 26 and GCaMP6s

   Depositor

   Fabio Mammano

   Article

   Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

   Insert

   GJB2 (GJB2 Human)
   

   Use

   Lentiviral

   Tags

   GCaMP6s

   Expression

   Mammalian

   Mutation

   Promoter

   Tight TRE promoter

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCW57-Cx26G45E-IRES-GCaMP6s

   Plasmid

   #188237

   Purpose

   3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 26 variant p.G45E and GCaMP6s

   Depositor

   Fabio Mammano

   Article

   Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

   Insert

   GJB2 (GJB2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   p.G45E

   Promoter

   Tight TRE promoter

   Available Since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCW57-mCherry-2A-BRD4 Iso AdelCTD

   Plasmid

   #137722

   Purpose

   Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)

   Depositor

   Scott Floyd

   Article

   Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

   Insert

   BRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Truncated at amino acid 1328

   Promoter

   Tight TRE promoter

   Available Since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRosa26-Acceptor (AG103)

   Plasmid

   #99632

   Purpose

   To insert the Tre-Tight or UAS ifgMosaic Donor vectors

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-nSpCas9-NoABE

    Plasmid

    #209043

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-nSpCas9-TadA8e

    Plasmid

    #209044

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TadA8e-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-nSpCas9-TadA8.20m

    Plasmid

    #209045

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8.20m is fused to the N-terminus of SpCas9(D10A)-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TadA8.20m-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-nSpCas9-evoCDA1

    Plasmid

    #209042

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    evoCDA1-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-nSpCas9-APOBEC3A

    Plasmid

    #209040

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    APOBEC3A-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-nSpCas9-NoCBE

    Plasmid

    #209038

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase) linked to ssDNA binding domain from RAD51 and uracil DNA glycosylase inhibitor (UGI)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-nSpCas9-APOBEC1

    Plasmid

    #209039

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    APOBEC1-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-nSpCas9-BEACON2

    Plasmid

    #209041

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where BEACON2 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    BEACON2-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUt-mPF-GFP

    Plasmid

    #199222

    Purpose

    LPUtopia-7 matching RMCE donor plasmid with GFP Positive-Feedback circuit (mPF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    rtTA-Advanced::P2A::EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRER-PhoCl-Insert

    Plasmid

    #219675

    Purpose

    Caging at lumenal N-ter of POI: TMEM-PhoCl-MCS in pTREtight2

    Depositor

    Helge Ewers

    Article

    Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

    Insert

    RER-PhoCl
    

    Use

    Tags

    PhoCl

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    June 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pTSA-38

    Plasmid

    #17328

    Depositor

    Takeshi Sano

    Article

    Sano et al Proc Natl Acad Sci U S A. 1995 Apr 11. 9

    Insert

    streptavidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Truncated to include only amino acids 16-133 of m…

    Promoter

    Available Since

    Feb. 25, 2008

    Availability

    Academic Institutions and Nonprofits only