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Showing: 341 - 360 of 19677 results

  3. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid
    #73498
    Purpose
    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus
    Depositor
    Bruce Conklin
    Publication
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB
    rtTA
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG and TRE3G
    Available since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73498-plasmid-map-sequence-id-364403

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  15. pUC gRNA Shuttle

    Plasmid
    #47024
    Purpose
    Encodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.
    Depositor
    Wayne Parrott
    Publication
    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:
    Insert
    gRNA Shuttle
    Use
    CRISPR; Cas9
    Expression
    Plant
    Mutation
    G to T cloning mutation at position 323
    Promoter
    Medicago truncatula U6.6
    Available since
    Sept. 3, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    47024-plasmid-map-sequence-id-67042

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  19. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV1)

    Viral prep
    #162379-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available since
    May 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  20. CMV-mito-LAR-GECO1.2

    Plasmid
    #61245
    Purpose
    Expresses LAR-GECO1.2 in the mitochondria in mammalian cells
    Depositor
    Robert Campbell
    Publication
    Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LAR-GECO1.2
    Tags
    a duplex of the mitochondrial targeting signal of…
    Expression
    Mammalian
    Mutation
    Substitutions relative to R-GECO1.2: N45I/A47R/E1…
    Promoter
    CMV
    Available since
    March 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61245-plasmid-map-sequence-id-238528

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Showing: 341 - 360 of 19677 results