We narrowed to 7,158 results for: ef1a

1. pGL3-EF1a-i53

   Plasmid

   #216243

   Purpose

   Express i53 in mammalian cells as a HDR enhancer

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   i53
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-mCherry

   Plasmid

   #114470

   Purpose

   mCherry Control

   Depositor

   Karl Deisseroth

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-pEF1a-tTAc

   Plasmid

   #172123

   Purpose

   Constitutive expression of InteinC-tTAC

   Depositor

   Chun Xu

   Article

   Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

   Insert

   InteinC-tTAc
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113343

   Purpose

   Expresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   p300core-dCas9-VP64-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX-EF1a_NKX2-1_Long

   Plasmid

   #221483

   Purpose

   NKX2-1 long isoform lentiviral overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. EF1a- gfp-2a-puro

   Plasmid

   #129443

   Purpose

   expression of GFP and Puro R

   Depositor

   Oskar Laur

   Insert

   gfp-puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF1a-BirA-V5-neo

   Plasmid

   #100548

   Purpose

   Expresses C-terminal V5-tagged BirA biotin ligase

   Depositor

   Jian Xu

   Article

   Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

   Insert

   C-terminal V5-tagged BirA
   

   Use

   Tags

   V5, His

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1a

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1a-HaloSFPQ

   Plasmid

   #166948

   Purpose

   Lentiviral plasmid expressing Halo-tagged SFPQ protein from the EF1a promoter

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   SFPQ (SFPQ Human)
   

   Use

   Lentiviral

   Tags

   Halo

   Expression

   Mutation

   Promoter

   eF1a

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-EF1aL-eGFP-W

   Plasmid

   #126686

   Purpose

   Constitutive, ubiquitous GFP expression.

   Depositor

   Darrell Kotton

   Article

   Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1aL

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT3-EF1A-mTert

    Plasmid

    #162555

    Purpose

    Overexpression of Tert

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    Overexpression of Tert (Tert Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF1A

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPV-EF1a-EGxxFP-iP-A

    Plasmid

    #204624

    Purpose

    Cloning vector for SSA vector (EGxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PEW-pEF1A-inactEGFP

    Plasmid

    #212009

    Purpose

    Cleavable backbone for constitutive expression of TCR alpha and beta chains through lentiviral transduction

    Depositor

    Catherine Wu

    Article

    Afeyan et al Nat Protoc. 2024 Nov 8. doi: 10.1038/s41

    Insert

    Inactivated eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF1a-FB-dCas9-puro

    Plasmid

    #100547

    Purpose

    Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Inserts

    N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
    puromycin
    

    Use

    CRISPR

    Tags

    FLAG, Biotin-acceptor-site (Avitag)

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-EF1aL-TagBFP-W

    Plasmid

    #126687

    Purpose

    Constitutive, ubiquitous blue fluorescence protein (BFP) expression.

    Depositor

    Darrell Kotton

    Article

    Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

    Insert

    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ef1aL

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lv EF1a dCas9-HA 2A Blast

    Plasmid

    #102812

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    dCas9-HA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A (dCas9)

    Promoter

    EF1a

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-FLEX-GT

    Plasmid

    #26198

    Depositor

    Edward Callaway

    Article

    Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    Enhanced green fluorescent protein, TVA (eGFP TVA is a mouse codon optimized version of an avian gene., Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    TVA mammalian codon optimized

    Promoter

    Available since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti_Ef1a:PE2-P2A-GFP

    Plasmid

    #184445

    Purpose

    Single EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

    Depositor

    Bobby Koeleman

    Article

    Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Ef1A

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSicoR-Ef1a-mCh

    Plasmid

    #31847

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV-EF1a-mCherryBCL11B-W

    Plasmid

    #208758

    Purpose

    Lentiviral vector expressing mCherry fused with BCL11B cDNA

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    BCL11B (BCL11B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-EF1a-BsdCas9-W

    Plasmid

    #67978

    Purpose

    Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-Cas9 cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Cas9 fused with Flag-tag and a NLS at the N termi…

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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