We narrowed to 1,261 results for: grna cloning vector

1. AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

   Plasmid

   #207880

   Purpose

   AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

   Depositor

   Yeh-Hsing Lao

   Article

   Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. lenti-EGFR-L858R-T790M-dual-epegRNA

   Plasmid

   #214100

   Purpose

   Lentiviral vector expressing epegRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two epegRNAs using independent U6 promoters.

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   EGFR L858R epegRNA/EGFR T790M epegRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

   Plasmid

   #112139

   Purpose

   Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.

   Depositor

   Erik Sontheimer

   Article

   Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:

   Inserts

   sgRNA scaffold
   Human-codon-optimized NmeCas9
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U1a and U6

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBBK04 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitHygR,AmpR)

   Plasmid

   #178988

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitHygromycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBBK02 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitKanR, AmpR)

   Plasmid

   #178986

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBBK10 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitURA3,AmpR)

   Plasmid

   #178994

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitURA3

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBBK08 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitLEU2,AmpR)

   Plasmid

   #178992

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitLEU2

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)

   Plasmid

   #178990

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LentiCRISPR-B_sgRNA_EXOSC6 (pP18(T)_B11-AVA4069)

   Plasmid

   #239304

   Purpose

   Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC6

   Depositor

   Andrei Alexandrov

   Article

   Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

   Insert

   U6-driven sgRNA targeting EXOSC6 (EXOSC6 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LentiCRISPR-B_sgRNA_EXOSC7 (pP18(T)_C4-AVA4066)

    Plasmid

    #239305

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC7

    Depositor

    Andrei Alexandrov

    Article

    Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

    Insert

    U6-driven sgRNA targeting EXOSC7 (EXOSC7 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LentiCRISPR-B_sgRNA_EXOSC8 (pP18(T)_B1-AVA4071)

    Plasmid

    #239306

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC8

    Depositor

    Andrei Alexandrov

    Article

    Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

    Insert

    U6-driven sgRNA targeting EXOSC8 (EXOSC8 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LentiCRISPR-B_sgRNA_EXOSC9 (pP18(T)_A7-AVA4072)

    Plasmid

    #239307

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC9

    Depositor

    Andrei Alexandrov

    Article

    Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

    Insert

    U6-driven sgRNA targeting EXOSC9 (EXOSC9 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LentiCRISPR-B_sgRNA_DIS3 (pP18(T)_A5-AVA4073)

    Plasmid

    #239308

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting DIS3

    Depositor

    Andrei Alexandrov

    Article

    Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

    Insert

    U6-driven sgRNA targeting DIS3 (DIS3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LentiCRISPR-B_sgRNA_C1D (pP18(T)_B9-AVA4070)

    Plasmid

    #239296

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting C1D

    Depositor

    Andrei Alexandrov

    Article

    Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

    Insert

    U6-driven sgRNA targeting C1D (C1D Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LentiCRISPR-B_sgRNA_EXOSC2 (pP18(T)_C3-AVA4067)

    Plasmid

    #239301

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC2

    Depositor

    Andrei Alexandrov

    Article

    Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

    Insert

    U6-driven sgRNA targeting EXOSC2 (EXOSC2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LentiCRISPR-B_sgRNA_EXOSC4 (pP18(T)_C11-AVA4065)

    Plasmid

    #239302

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC4

    Depositor

    Andrei Alexandrov

    Article

    Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

    Insert

    U6-driven sgRNA targeting EXOSC4 (EXOSC4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LentiCRISPR-B_sgRNA_EXOSC5 (pP18(T)_B12-AVA4068)

    Plasmid

    #239303

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC5

    Depositor

    Andrei Alexandrov

    Article

    Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

    Insert

    U6-driven sgRNA targeting EXOSC5 (EXOSC5 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. scAAV-sgRNA-GFP

    Plasmid

    #177935

    Purpose

    Expresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic mice

    Depositor

    Roland Rad

    Article

    Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LentiCRISPR-B_2xsgRNAs_UPF3A/UPF3B (pAVA3129)

    Plasmid

    #239329

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting UPF3A and UPF3B

    Depositor

    Andrei Alexandrov

    Article

    Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

    Insert

    U6-driven sgRNA1 targting UPF3A and 7SK-driven sgRNA2 targeting UPF3B (UPF3B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOTTC1642 - pAAV EF1a EGFP-KASH with mTACR1 gRNAs

    Plasmid

    #195018

    Purpose

    An adeno-associated viral vector expressing eGFP-KASH and two guides targeting mouse TACR1

    Depositor

    Christopher Richie

    Article

    Belilos et al bioRxiv [Preprint]. 2023 Oct 9:2023.02.0

    Inserts

    CCGTATAGGCGGCTGCCCAA
    EGFP-KASH
    TTCCGTGGTGGGCAACGTAG
    

    Use

    AAV

    Tags

    KASH domain of Nesprin2

    Expression

    Mutation

    Promoter

    EF1a, hU6, and mU6

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge