We narrowed to 151,823 results for: ins

Showing: 341 - 360 of 151823 results

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ActinS14V.CIB.GFP

Plasmid

#133620

Purpose

Expresses beta actin S14V - CIBN - GFP protein fusion

Depositor

Robert Hughes

Article

Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

Insert

betaActin - CIB

Tags

GFP

Expression

Mammalian

Promoter

cmv

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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FR_HNF1B_A263insGG

Plasmid

#31103

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF1B (HNF1B Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

mutation A263insGG

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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twinStrep_FLAG_aMFx1 (4a)

Plasmid

#238904

Purpose

Plasmid insert contains the aMFx1, FLAG and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

twinStrep_FLAG_aMFx1

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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twinStrep_HA_aMFx1 (4a)

Plasmid

#238905

Purpose

Plasmid insert contains the aMFx1, HA and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

twinStrep_HA_aMFx1

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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twinStrep_aMFx1 (4a)

Plasmid

#238906

Purpose

Plasmid insert contains the aMFx1 and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

twinStrep_aMFx1

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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makeTCR_amilCP_control_insert

Plasmid

#233942

Purpose

control for makeTCR assembly

Depositor

Michael Platten

Insert

amilCP

Expression

Bacterial

Promoter

BBa_J23110

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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insulator_seq_barcode_vector

Plasmid

#221458

Purpose

Vector for generating a barcode library for insulator-seq.

Depositor

Maria Cristina Gambetta

Article

Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

Type

Empty backbone

Use

Synthetic Biology and Unspecified

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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insulator_seq_UENU_donor

Plasmid

#221459

Purpose

Vector from which reporter gene 3'UTRs, an enhancer, and a neutral DNA spacer are cloned for assembling an insulator-seq reporter library.

Depositor

Maria Cristina Gambetta

Article

Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

Insert

3'UTR-OpIE2 enhancer-neutral DNA spacer-3'UTR

Use

Synthetic Biology

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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insulator_seq_reporter_vector

Plasmid

#221460

Purpose

Vector containing eGFP and mCherry reporter genes, for assembling an insulator-seq reporter library.

Depositor

Maria Cristina Gambetta

Article

Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

Insert

gypsy insulator-EGFP-neutral spacer DNA-mCherry

Use

Synthetic Biology

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAGM9121_TwinStrep

Plasmid

#153513

Purpose

TwinStrep C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

TwinStrep

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pGEM_HcBOP1ainsitu

Plasmid

#136529

Purpose

Template for generating in situ hybridization probe

Depositor

Junko Kyozuka

Article

Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

Insert

Houttuynia cordata BLADE-ON-PETIOLE homolog1a

Use

Cloning vector

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pGEM_ZmaBOP1binsitu

Plasmid

#136531

Purpose

Template for generating in situ hybridization probe

Depositor

Junko Kyozuka

Article

Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

Insert

Zoysia matrella BLADE-ON-PETIOLE homolog1b

Use

Cloning vector

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pGEM_ZmaBOP2binsitu

Plasmid

#136532

Purpose

Template for generating in situ hybridization probe

Depositor

Junko Kyozuka

Article

Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

Insert

Zoysia matrella BLADE-ON-PETIOLE homolog2b

Use

Cloning vector

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pGEM_HcBOP1binsitu

Plasmid

#136530

Purpose

Template for generating in situ hybridization probe

Depositor

Junko Kyozuka

Article

Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

Insert

Houttuynia cordata BLADE-ON-PETIOLE homolog1b

Use

Cloning vector

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_RIT1_p.T76insTDLT

Plasmid

#82916

Purpose

Gateway Donor vector containing RIT1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RIT1 (RIT1 Human)

Use

Gateway entry vector

Mutation

TDLT insertion at residue T76

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTD-CTwinStrep

Plasmid

#45939

Purpose

use to create C-terminal Twin-Strep-tag fusion proteins

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Tags

Twin-Strep

Expression

Bacterial

Promoter

Trc (lactose/IPTG inducible)

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pTDpelB-C_sfYFPTwinStrep

Plasmid

#45944

Purpose

expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Insert

synthetic sfYFP (codon usage adapted to P.putida KT2440)

Tags

PelB export sequence and Twin-Strep-tag

Expression

Bacterial

Promoter

lacIq/Ptrc

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pTDpelB-NTwinStrep

Plasmid

#45940

Purpose

use to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequence

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Tags

6xHis, PelB, and Twin-Strep

Expression

Bacterial

Promoter

Trc (lactose/IPTG inducible)

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pTDpelB-CTwinStrep

Plasmid

#45941

Purpose

use to create N-terminal PelB and C-terminal Twin-Strep-tagged fusion proteins for periplasmic translocation via PelB signal sequence

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Tags

PelB and Twin-Strep

Expression

Bacterial

Promoter

Trc (lactose/IPTG inducible)

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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InsRA-eGFP

Plasmid

#79795

Purpose

Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

InsRA (INSR Human)

Tags

eGFP extracellular

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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