We narrowed to 144,361 results for: ins

1. pGEM_ZmaBOP1binsitu

   Plasmid

   #136531

   Purpose

   Template for generating in situ hybridization probe

   Depositor

   Junko Kyozuka

   Article

   Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

   Insert

   Zoysia matrella BLADE-ON-PETIOLE homolog1b
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEM_ZmaBOP2binsitu

   Plasmid

   #136532

   Purpose

   Template for generating in situ hybridization probe

   Depositor

   Junko Kyozuka

   Article

   Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

   Insert

   Zoysia matrella BLADE-ON-PETIOLE homolog2b
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEM_HcBOP1binsitu

   Plasmid

   #136530

   Purpose

   Template for generating in situ hybridization probe

   Depositor

   Junko Kyozuka

   Article

   Toriba et al Curr Biol. 2020 Feb 3;30(3):509-516.e3.

   Insert

   Houttuynia cordata BLADE-ON-PETIOLE homolog1b
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223_RIT1_p.T76insTDLT

   Plasmid

   #82916

   Purpose

   Gateway Donor vector containing RIT1, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Matthew Meyerson, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   RIT1 (RIT1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   TDLT insertion at residue T76

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTD-NTwinStrep_Sm

   Plasmid

   #45937

   Purpose

   use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Use

   Tags

   6xHis and Twin-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Trc (lactose/IPTG inducible)

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. proinsulin-EYFP

   Plasmid

   #218952

   Purpose

   Expresses proinsulin fused to EYFP in bacteria

   Depositor

   Benjamin Glick

   Insert

   proinsulin-EYFP
   

   Use

   Tags

   proinsulin

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTD-NTwinStrep_Km

   Plasmid

   #45938

   Purpose

   use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Use

   Tags

   6xHis and Twin-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Trc (lactose/IPTG inducible)

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-U6insert-EF1puro

   Plasmid

   #104537

   Purpose

   A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

   Depositor

   George Church

   Article

   Kalhor et al Science. 2018 Aug 9. pii: science.aat980

   Type

   Empty backbone

   Use

   CRISPR, Mouse Targeting, and Synthetic Biology ; …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-calpainsensor

   Plasmid

   #36182

   Purpose

   detection of activity of ubiquitous calpains

   Depositor

   Isabelle Richard

   Article

   Stockholm et al J Mol Biol. 2005 Feb 11;346(1):215-22. E

   Insert

   alpha-Fodrin cleavage site (Sptan1 Mouse)
   

   Use

   Tags

   eCFP and eYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTD-CTwinStrep

    Plasmid

    #45939

    Purpose

    use to create C-terminal Twin-Strep-tag fusion proteins

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. InsRA-eGFP

    Plasmid

    #79795

    Purpose

    Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    InsRA (INSR Human)
    

    Use

    Tags

    eGFP extracellular

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. PDZD7-TwinStrepII

    Plasmid

    #83198

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Article

    Morgan et al Elife. 2016 Aug 15;5:e18312. doi: 10.755

    Insert

    PDZD7 (Pdzd7 Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. DFNB31-TwinStrepII

    Plasmid

    #83196

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Insert

    DFNB31 (Whrn Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. USH1CA1-TwinStrepII

    Plasmid

    #83202

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Insert

    USH1C (Ush1c Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTDpelB-C_sfYFPTwinStrep

    Plasmid

    #45944

    Purpose

    expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Insert

    synthetic sfYFP (codon usage adapted to P.putida KT2440)
    

    Use

    Tags

    PelB export sequence and Twin-Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq/Ptrc

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. ins:CFP-NTR

    Plasmid

    #66593

    Purpose

    Specific and conditional cell ablation of beta cells in the zebrafish pancreas

    Depositor

    Didier Stainier

    Article

    Curado et al Dev Dyn. 2007 Apr . 236(4):1025-35.

    Insert

    Insulin promotor driving CFP-nitroreductase
    

    Use

    Zebrafish expression

    Tags

    CFP fused with N-terminus of nitroreductase

    Expression

    Mutation

    Promoter

    Zebrafish Insulin promoter (approximately 1.2kb)

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pTDpelB-NTwinStrep

    Plasmid

    #45940

    Purpose

    use to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequence

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis, PelB, and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. kanMX6-ins2

    Plasmid

    #195039

    Purpose

    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPINS-UBE3C

    Plasmid

    #66711

    Purpose

    Expresses UBE3C HECT domain in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Insert

    KiAA0010 (UBE3C )
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

20. SUMO_TwinStrep_Cas7-11_crRNA

    Plasmid

    #197612

    Purpose

    For protein purification of Cas7-11

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2022 Nov 25;378(6622):874-881.

    Insert

    SUMO_TwinStrep_Cas7-11 with crRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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