We narrowed to 1,038 results for: transfection
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Plasmid#137764PurposeControl plasmid for immunoprecipitation of DDK/FLAG-tagged proteins. Also a good control for transfection efficiency.DepositorInsertGFP
TagsDDKExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
BCL9-BirA*
Plasmid#109147PurposeTo generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cellsDepositorAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT C41CA_GFP 150TAG
Plasmid#140017PurposePlasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAGPromoterEF1Available SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIhh742bp-Luc
Plasmid#53605PurposeReporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterAvailable SinceJune 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT sfGFP 150TAG
Plasmid#154771Purposeplasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAGPromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIhh1.3K-Luc
Plasmid#53604PurposeReporter construct containing a 1.3 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterAvailable SinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
C211
Plasmid#174360PurposeFor expressing siRNA-resistant human PABPC1 mutant (I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K) in human cell lines via transfectionDepositorInsertPABPC1 (PABPC1 Human)
TagsFLAGExpressionMammalianMutationI110L, D111E, D117E, A121G, G139A, Y140F, T147S, …Available SinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDOE11 mas:mTq2 transient
Plasmid#124248PurposeAllows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected plant cells.DepositorTypeEmpty backboneExpressionPlantAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIhh715bp-Luc
Plasmid#53606PurposeReporter construct containing a 715 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterAvailable SinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBOB-3xFlag-hGpr75-p.Gln234*
Plasmid#236748PurposeExpress 3xFlag N-terminal tagged human GPR75 p.Gln234* mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.DepositorInsertGPR75 (GPR75 Human)
UseLentiviralTags3xFlagExpressionMammalianMutationGln234 to STOP codon, CAA to TAAAvailable SinceJuly 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBOB-3xFlag-hGpr75-p.Ala110fs
Plasmid#236747PurposeExpress 3xFlag N-terminal tagged human GPR75 p.Ala110fs mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.DepositorInsertGPR75 (GPR75 Human)
UseLentiviralTags3xFlagExpressionMammalianMutationAla110fs, deletion of GCCAGTAAvailable SinceJuly 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
FHpHG_mTent5c
Plasmid#221440PurposeTransfection into the cells for the reporter or qPCR assay.DepositorAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV-TO-g0 (FLP-IN)
Plasmid#236120PurposePlasmid encoding g0 guide RNA under control of CMV promoter with two TetR binding sites, used to equalize plasmid masses for transfectionDepositorInsertg0
ExpressionMammalianPromoterCMV promoter with two TetR binding sitesAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIhh4.5k-Luc
Plasmid#53603PurposeReporter construct containing a 4.5 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterAvailable SinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
LmexBE_v1
Pooled Library#236783PurposeA genome-wide loss-of-function CRISPR base editing library designed to introduce STOP codons within the first 50% of coding sequences in the genome of Leishmania mexicana.DepositorSpeciesLeishmania mexicanaUseCRISPRAvailable SinceApril 1, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCXWB-EBNA1
Plasmid#37624PurposeNon-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmidsDepositorAvailable SinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
Synapsin sHRPa-NRX in FSW lentiviral vector
Plasmid#73147PurposeLarge fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.DepositorInsertsHRPa-NRX
UseLentiviralTags2x HA tag and Flag tagExpressionMammalianPromoterSynapsinAvailable SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PuroR-T2A-eGFP
Plasmid#234734PurposePuromycin resistance/eGFP cassette in mammalian cells (transfection marker)DepositorInsertPuroR-T2A-eGFP
ExpressionMammalianAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry
Plasmid#108685PurposeStrong CAG promoter with mCherry expression for transfection efficacy screeningDepositorInsertmCherry
ExpressionMammalianPromoterCAGAvailable SinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
gRNA-CasRx_SD1-pSV40-TagBFP
Plasmid#221004PurposeTransiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.DepositorInsertCasRx 30nt processed direct repeat with SD1 mutation
UseCRISPRExpressionMammalianMutationDR1 A>TPromoterU6Available SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only