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Addgene

We narrowed to 19,699 results for: ACE

Showing: 7161 - 7200 of 19699 results
  1. qTAG-C-Solo-mStayGold-VIM

    Plasmid
    #227302
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the VIM locus. For intermediate filament visualization. To be co-transfected with sgRNA plasmid px330-PITCh-VIM (Addgene #227301)
    Depositor
    Insert
    VIM Homology Arms flanking a mStayGold Tag (VIM Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. qTAG-C-mNeon-Puro-TOMM20

    Plasmid
    #207790
    Purpose
    Donor template for mNeon-2A-Puro insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)
    Depositor
    Insert
    TOMM20 Homology Arms flanking a mNeon-Puro Cassette (TOMM20 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pCK340

    Plasmid
    #192639
    Purpose
    Expresses dSpG and MCP-SoxS(R93A/S101A) on p15A-CmR
    Depositor
    Inserts
    Sp.pCas9-dSpG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    SoxS has R93A and S101A mutations and dSpG has se…
    Promoter
    BBa_J23107 and Sp.pCas9
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pPtGE34

    Plasmid
    #107932
    Purpose
    Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation
    Depositor
    Inserts
    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    Use
    CRISPR and Synthetic Biology; Episomal vector for…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. qTAG-C-Solo-mStayGold-TOMM20

    Plasmid
    #227307
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)
    Depositor
    Insert
    TOMM20 Homology Arms flanking a mStayGold Tag (TOMM20 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pcDNA5/FRT/TO-GAlphasS-RLuc8

    Plasmid
    #140980
    Purpose
    Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Insert
    GAlphasS-RLuc8
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. CMV-mito-CAR-GECO1

    Plasmid
    #46022
    Purpose
    Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging
    Depositor
    Insert
    CAR-GECO1
    Use
    Tags
    a duplex of the mitochondrial targeting signal of…
    Expression
    Mammalian
    Mutation
    R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P
    Promoter
    CMV
    Available since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only
  8. pCPP5912

    Plasmid
    #128703
    Purpose
    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone
    Depositor
    Insert
    shcE-avrE1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Three synonymous mutations Ala 362 (GCT-GCC), Ala…
    Promoter
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. qTAG-N-Blast-3xHA-LMNB1

    Plasmid
    #207778
    Purpose
    Donor template for Blast-2A-3xHA insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Insert
    LMNB1 Homology Arms flanking a Blast-3xHA Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  10. qTAG-N-Puro-sTagRFP-TUBA1B

    Plasmid
    #207769
    Purpose
    Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Insert
    TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  11. qTAG-N-Solo-mStayGold-CETN2

    Plasmid
    #227292
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the CETN2 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CETN2 (Addgene #227291)
    Depositor
    Insert
    CETN2 Homology Arms flanking a mStayGold Tag (CETN2 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. pLenti4/TO-hEPO

    Plasmid
    #50437
    Purpose
    express human erythropoietin under the control of tetracycline-regulatable CMV promoter using lentiviral delivery system
    Depositor
    Insert
    EPO (EPO Human)
    Use
    Lentiviral; Tetracycline-regulatable (t-rex)
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/TO
    Available since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
  13. pB1H2 UV5 Zif268-cons (2-hybrid bait plasmid)

    Plasmid
    #128164
    Purpose
    UV5 promoter drives expression of Zif268-consensus peptide fusion. Pair with pGHUC w-ERBIN as a positive control (turns on HIS3/GFP).
    Depositor
    Insert
    10 amino acid glycine/serine linker + ERBIN PDZ consensus ligand (WETWV)
    Use
    Synthetic Biology
    Tags
    FLAG and zif268
    Expression
    Bacterial
    Mutation
    Promoter
    UV5
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
  14. qTAG-C-Solo-mStayGold-CLTC

    Plasmid
    #227313
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)
    Depositor
    Insert
    CLTC Homology Arms flanking a mStayGold Tag (CLTC Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 7161 - 7200 of 19699 results