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pCDNA3_HMOX1-V5-APEX2

Plasmid

#85823

Purpose

Expressed C-term tagged APEX2 on HMOX1 in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

Insert

HMOX1-V5-APEX2 (HMOX1 Synthetic, Human)

Tags

APEX2 and V5

Expression

Mammalian

Promoter

CMV-F

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pGM8

Plasmid

#220166

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TSPO (TSPO Human)

Use

Lentiviral

Tags

GFP11

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-Narg2

Plasmid

#49247

Purpose

mammalian expression of flag-tagged human Narg2

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Narg2 (NARG2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only
3xFlag-HaloTag-SUZ12 HDR - pDY082

Plasmid

#171110

Purpose

Homology directed repair plasmid for generating HaloTag-SUZ12 cells

Depositor

Thomas Cech

Article

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Insert

SUZ12 (SUZ12 Human)

Tags

HaloTag

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCK-Drosha(UniProt)-FLAG-mEGFP

Plasmid

#164691

Purpose

Express Drosha-FLAG-mEGFP in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

Drosha (DROSHA Human)

Tags

FLAG and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-Neuromodulin-IRES-Puromycin

Plasmid

#134666

Purpose

Expresses EGFP-tagged plasma membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Neuromodulin (GAP43 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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Sema6c-AP-His

Plasmid

#72041

Purpose

Expresses the extracellular region of the Sema6C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6c (Sema6c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pH2R-mCherry-N1

Plasmid

#84326

Purpose

Histamine-2 receptor tagged with mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H2R (HRH2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Sema6d.4-AP-His

Plasmid

#72044

Purpose

Expresses the extracellular region of the Sema6D, isoform 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6d.4 (Sema6d Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Glt1-BioID2-BioID2-HA

Plasmid

#176745

Purpose

Expresses Glt-1 fused with BioID2 at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Glt1-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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Sema3c(L)-AP-His

Plasmid

#72015

Purpose

Expresses the Sema3C protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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PZac2.1 hSyn1-HA-BioID2-SAPAP3

Plasmid

#176857

Purpose

Expresses SAPAP3 protein fused with BioID2 at the N-terminus in neurons

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

HA-BioID2-SAPAP3

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

hSyn1

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-human NMyc-humanER-IRES-mCherry

Plasmid

#73571

Purpose

Mammalian retroviral expression of NMyc-ER

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

NMyc (MYCN Human)

Use

Retroviral

Tags

Human ER and IRES-mCherry

Expression

Mammalian

Promoter

MSCV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pBSSK p85 alpha (delta iSH2)

Plasmid

#13432

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (delta iSH2) (PIK3R1 Human)

Tags

flag

Expression

Bacterial

Mutation

Dominant negative p85 alpha. The p110 alpha bindi…

Available Since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only
Plxnb1(L)-Fc-His

Plasmid

#72126

Purpose

Expresses the entire extracellular region of the PlexinB1 protein (ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnb1 (Plxnb1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA pcDNA3

Plasmid

#206051

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS2

Plasmid

#127127

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS2 (RPS2 Human)

Use

Lentiviral

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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YFP-Rpp29-C1

Plasmid

#134545

Purpose

Expresses YFP-Rpp29 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Rpp29 (POP4 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pKG-GW1

Plasmid

#203163

Purpose

Yeast expression vector; To express proteins under control of TDH3 promoter and terminator using leucine selection

Depositor

William Sessa

Article

Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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Sema3e(L)-AP-His

Plasmid

#72019

Purpose

Expresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3e (Sema3e Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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