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pcDNA3-LifeAct-FRB-DMVF-181-N

Plasmid

#87610

Purpose

FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-FRB-DMVF181-N

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Tet3

Plasmid

#60940

Purpose

Expresses Tet3 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 3

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pGwf

Plasmid

#22517

Depositor

Christopher Buck

Article

Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCGN

Plasmid

#53395

Purpose

mammalian cell expression vector with HA-tag

Depositor

Winship Herr

Article

Tanaka et al Cell. 1990 Feb 9;60(3):375-86.

Insert

HA tag

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

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phGf

Plasmid

#22516

Depositor

Christopher Buck

Article

Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pDisplay-LAP2-CFP-TM

Plasmid

#34842

Depositor

Alice Ting

Insert

LAP2-CFP

Tags

HA and myc

Promoter

CMV

Available Since

Feb. 15, 2012

Availability

Academic Institutions and Nonprofits only

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ZE25 Stbm-pCS2+

Plasmid

#17067

Depositor

Randall Moon

Article

Park et al Nat Cell Biol. 2002 Jan;4(1):20-5.

Insert

Stbm (vangl2 Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pET28c-F30-2xdBroccoli

Plasmid

#66843

Purpose

Expresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cells

Depositor

Samie Jaffrey

Article

Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

Insert

Two dimeric Broccoli aptamers in the F30 scaffold

Expression

Bacterial

Promoter

T7

Available Since

July 13, 2015

Availability

Academic Institutions and Nonprofits only

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pSFV3-lacZ

Plasmid

#92074

Purpose

This is an expression plasmid for production of recombinant genomic SFV RNA encoding beta-galactosidase.

Depositor

Henrik Garoff

Article

Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

Insert

lacZ

Use

Lacz expression plasmid, for run-off transcriptio…

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-MCS2

Plasmid

#46954

Purpose

Cloning plasmid for the generation of Knock-In in human cells using rAAV.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Type

Empty backbone

Use

AAV

Available Since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-CMV-DsRed-IRES-d1EGFP

Plasmid

#41942

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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Construct 32 - NFKBRp_mKate2_2xNLS_p2a-puroR

Plasmid

#82024

Purpose

Lentiviral construct for building stable cell lines expressing mKate2 in the presence of TNFa via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

mKate2-2xNLS

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-CMV-DsRed-IRES-d24EGFP

Plasmid

#41944

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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FUGW-pHRed

Plasmid

#65742

Purpose

Lentiviral vector for pHRed expression in mammalian cells using the hUbiC promoter

Depositor

Mathew Tantama

Article

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

pHRed

Use

Lentiviral

Expression

Mammalian

Promoter

hUbiC

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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Construct 28 -hUBCp_TetR_p2a_LacI_p2a_ZeoR

Plasmid

#81253

Purpose

Lentiviral construct for building stable cell lines expressing TetR and LacI via Zeocin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

TetR and LacI

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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ptdTomato-SMCR8

Plasmid

#83438

Purpose

Expresses SMCR8-tdTomato in mammalian cells

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

Human SMCR8 (SMCR8 Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pICE

Plasmid

#46960

Purpose

Plasmid for cloning and constitutive or doxycycline-inducible expression of cDNAs. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Type

Empty backbone

Expression

Mammalian

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-CMV-DsRed-IRES-d4EGFP

Plasmid

#41943

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pTAL7b

Plasmid

#48706

Purpose

TALEN expression in mammalian cells

Depositor

Boris Greber

Article

Frank et al BMC Genomics. 2013 Nov 9;14(1):773.

Type

Empty backbone

Use

TALEN

Tags

FokI nuclease

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-TRE-DsRed-IRES-d24EGFP

Plasmid

#41945

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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