We narrowed to 21,706 results for: pET

1. pET His6 High pI protein 69 TEV LIC cloning vector (2-HpI-69)

   Plasmid

   #55212

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, High pI protein 69, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET FLAG TEV cloning vector with BioBrick polycistronic restriction sites (9L)

   Plasmid

   #48288

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   FLAG

   Expression

   Bacterial

   Available Since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET Mocr TEV expression vector with BioBrick polypromoter restriction sites (14-W)

   Plasmid

   #48315

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   Mocr

   Expression

   Bacterial

   Available Since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His

   Plasmid

   #222288

   Purpose

   Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal GFP and His tag in E.coli for purification.

   Depositor

   Anne Straube

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Insert

   KIF1C (KIF1C Human)
   

   Tags

   6xHis, GFP, and GST

   Expression

   Bacterial

   Mutation

   stalk region only amino acids 642 to 922

   Promoter

   T7

   Available Since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (I224A/L227A/W276A/I279A; deltaLIR1+3)

   Plasmid

   #223754

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   BCL2L13 soluble part (BCL2L13 Human)
   

   Tags

   Thrombin cleavage-GST

   Expression

   Bacterial

   Mutation

   expresses aa 1-465, with I224A/L227A/W276A/I279A;…

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (W276A/I279A/I307A/V310A; deltaLIR1+4)

   Plasmid

   #223755

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   BCL2L13 soluble part (BCL2L13 Human)
   

   Tags

   Thrombin cleavage-GST

   Expression

   Bacterial

   Mutation

   expresses aa 1-465, with W276A/I279A/I307A/V310A;…

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (Y213A/I216A/W276A/I279A; deltaLIR1+2)

   Plasmid

   #223752

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   BCL2L13 soluble part (BCL2L13 Human)
   

   Tags

   Thrombin cleavage-GST

   Expression

   Bacterial

   Mutation

   expresses aa 1-465, with Y213A/I216A/W276A/I279A;…

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETDuet1_NIX(1-182aa)-THROMBIN-GST (E72A/L75A/D77A/E81A; deltaWIPI2)

   Plasmid

   #223753

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   NIX (BNIP3L) soluble part (BNIP3L Human)
   

   Tags

   Thrombin cleavage-GST

   Expression

   Bacterial

   Mutation

   expresses aa 1-182, with E72A/L75A/D77A/E81A; del…

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-His10-BTLAicd (AA190-289, Y243F, Y257F, Y282F)-TwinStrep

   Plasmid

   #180806

   Purpose

   Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y243F, Y257F, Y282F)-TwinStrep

   Depositor

   Enfu Hui

   Article

   Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   BTLAint (AA190-289, Y243F, Y257F, Y282F)-TwinStrep (BTLA Human)
   

   Expression

   Bacterial

   Mutation

   BTLA (Y243F, Y257F, Y282F)

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-His10-BTLAicd (AA190-289, Y226F, Y257F, Y282F)-TwinStrep

    Plasmid

    #180807

    Purpose

    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y257F, Y282F)-TwinStrep

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    BTLAint (AA190-289, Y226F, Y257F, Y282F)-TwinStrep (BTLA Human)
    

    Expression

    Bacterial

    Mutation

    BTLA (Y226F, Y257F, Y282F)

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y282F)-TwinStrep

    Plasmid

    #180808

    Purpose

    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y282F)-TwinStrep

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    BTLAint (AA190-289, Y226F, Y243F, Y282F)-TwinStrep (BTLA Human)
    

    Expression

    Bacterial

    Mutation

    BTLA (Y226F, Y243F, Y282F)

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y257F)-TwinStrep

    Plasmid

    #180809

    Purpose

    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y257F)-TwinStrep

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    BTLAint (AA190-289, Y226F, Y243F, Y257F)-TwinStrep (BTLA Human)
    

    Expression

    Bacterial

    Mutation

    BTLA (Y226F, Y243F, Y257F)

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (wild type)

    Plasmid

    #153439

    Purpose

    E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Tags

    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-28b-T7-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1916)

    Plasmid

    #114076

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) with C-terminal NLS and His-tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a-RR (S542R/K607R) with C-terminal NLS and His-tag
    

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Mutation

    S542R and K607R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET MYFQSNA tagged cloning vector with BioBrick polycistronic restriction sites (9U)

    Plasmid

    #48293

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    MYFQSNA

    Expression

    Bacterial

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L536A/pET28HMT

    Plasmid

    #111400

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Tags

    His Tag and MBP tag

    Expression

    Bacterial

    Mutation

    Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L540A

    Promoter

    T7

    Availability

    Academic Institutions and Nonprofits only

    

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17. Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D/pET28HMT

    Plasmid

    #111451

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Tags

    His Tag and MBP tag

    Expression

    Bacterial

    Mutation

    Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D

    Availability

    Academic Institutions and Nonprofits only

    

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18. sortase A pentamutant (eSrtA) in pET29

    Plasmid

    #75144

    Purpose

    evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

    Depositor

    David Liu

    Article

    Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

    Insert

    sortase A pentamutant
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T. Deleted amino a…

    Promoter

    T7

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-NLS-Cas9-6xHis

    Plasmid

    #62934

    Purpose

    Expression of NLS-Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    NLS-Cas9-6xHis
    

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21a(+)-Histag-mCherry (527)

    Plasmid

    #70719

    Purpose

    Expression vector for mCherry purification

    Depositor

    Richard Murray

    Article

    Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

    Insert

    mCherry
    

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET-dCas9-VP64-6xHis

    Plasmid

    #62935

    Purpose

    Expression of dCas9-VP64-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    dCas9-VP64
    

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A and H840A amino acid changes render Cas9 nuc…

    Promoter

    T7

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET21-GFP-AURKA-mCherry

    Plasmid

    #99876

    Purpose

    Expression of GFP-AURKA-mCherry for protein purification in bacteria

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP and mCherry

    Expression

    Bacterial

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET LIC cloning vector (2A-T)

    Plasmid

    #29665

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET-28b-Cas9-His

    Plasmid

    #47327

    Purpose

    For in vitro expression and purification of Cas9 protein

    Depositor

    Alex Schier

    Article

    Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His

    Expression

    Bacterial

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET-alpha-synuclein-BiFC

    Plasmid

    #87855

    Purpose

    pETDUET-1 based vector for BiMolecular Fluorescence Complementation based assays of alpha-synuclein oligomerisation

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

    Inserts

    alpha-synuclein-VC155
    alpha-synuclein-VN154
    

    Tags

    C-terminal fragment of mVenus BiFC protein and N-…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEThT-MAHS delta MTP

    Plasmid

    #90032

    Purpose

    To express His-tagged MAHS in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

    Insert

    MAHS
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Deletion of mitochondria targeting peptide

    Promoter

    T7

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-Cas9-NLS-6xHis

    Plasmid

    #62933

    Purpose

    Expression of Cas9-NLS-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9-NLS-6xHis
    

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET151-GST-PP-RPN13 (19-132)

    Plasmid

    #73742

    Purpose

    Expression of human RPN13 PRU domain in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN13 (ADRM1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    PRU domain only (residues 19-132)

    Promoter

    T7

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

29. pET-15b-UbcH5b/2419

    Plasmid

    #11475

    Depositor

    Allan Weissman

    Article

    Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

    Insert

    UbcH5b (UBE2D2 Human)
    

    Expression

    Bacterial

    Mutation

    n/a

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

30. pET28a-6XHis-SUMO-SIRT2

    Plasmid

    #102622

    Purpose

    Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    SIRT2 (SIRT2 Human)
    

    Tags

    6XHis-SUMO

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-37 and 357-389

    Available Since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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