We narrowed to 9,466 results for: CAG

1. pCH68 (dAsCas12a-3xKRAB piggyBac)

   Plasmid

   #217334

   Purpose

   piggyBac expression of dAsCas12a-3xKRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   dAsCas12a
   

   Use

   CRISPR

   Tags

   nucleoNLS-3xKRAB-3xHA-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   E993A

   Promoter

   CAG

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. #2.4 pre-G-SynapShot-Myc

   Plasmid

   #214722

   Purpose

   Expresses green #2.4 pre-SynapShot and Myc tag

   Depositor

   Won Do Heo

   Article

   Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

   Insert

   #2.4 pre-G-SynapShot
   

   Use

   AAV

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAT003

   Plasmid

   #171628

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-TRAC
   mNeon
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CAG and EF1-a

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Bxb1-GA_AIO-GZV-SynZiFTR_mGL donor

   Plasmid

   #229788

   Purpose

   Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible mGreenLantern expression

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mGreenLantern; ZF10-NS3-p65
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   ZF10 and CAG

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. SQT1665

   Plasmid

   #78744

   Purpose

   Human expression plasmid for LbCpf1-NLS-3xHA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCX-SpiCee-NLS

   Plasmid

   #140900

   Purpose

   mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-NLS
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-FLEX-EGFP-mir30(Scn9a)

   Plasmid

   #79672

   Purpose

   Cre-dependent knockdown of Scn9a (Nav1.7)

   Depositor

   Scott Sternson

   Article

   Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

   Insert

   EGFP (Scn9a Mouse, jellyfish)
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. SQT1659

   Plasmid

   #78743

   Purpose

   Human expression plasmid for AsCpf1-NLS-3xHA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   mammalian codon-optimized Cpf1-NLS-3xHA
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Double UP mNeon to mScarlet Rac1-V12

   Plasmid

   #125136

   Purpose

   Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-V12 (Constitutively Active).

   Depositor

   Erik Dent

   Article

   Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

   Insert

   mNeon/mScarletP2A Rac1-V12
   

   Expression

   Mammalian

   Mutation

   Removed Not1 site in mScarlet. 12th AA in Rac1 ch…

   Promoter

   CAG

   Available Since

   May 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAGK61

    Plasmid

    #228121

    Purpose

    Express node 1467 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1467, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Expression

    Mammalian

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCX-SpiCee-NES

    Plasmid

    #140901

    Purpose

    mammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    SpiCee-NES
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB-Neo-Mito-DsRed

    Plasmid

    #210328

    Purpose

    Overexpression of Mito-DsRed

    Depositor

    Li Yu

    Article

    Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

    Insert

    Mito-DsRed
    

    Expression

    Mammalian

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Bxb1-GA_AIO-TetOn_mScarlet_Down-Tandem

    Plasmid

    #229792

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    TRE and CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIG2-Flag-mNuak1 WT

    Plasmid

    #168507

    Purpose

    For expression of Nuak1 (AMPK-related kinase also called ARK5/Omphk1)

    Depositor

    Franck Polleux

    Article

    Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

    Insert

    Nuak1 (Nuak1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCX-NTOM-SpiCee

    Plasmid

    #140902

    Purpose

    mammalian expression of NTOM-SpiCee, a mitochondria-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    NTOM-SpiCee
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPBbsr2-Lifeact-miRFP703

    Plasmid

    #178530

    Purpose

    Expresses Lifeact-miRFP703 in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Lifeact-miRFP703
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCX-Lyn-SpiCee

    Plasmid

    #140837

    Purpose

    mammalian expression of Lyn-SpiCee, a lipid raft-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    Lyn-SpiCee
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-GfaABC1D-iCreV-4x6T

    Plasmid

    #196412

    Purpose

    Astrocytic expression of light-inducible iCreV recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    iCreV
    

    Use

    AAV; Astrocyte-selective

    Expression

    Mammalian

    Promoter

    CAG and GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-FLEX-VAMP2:HRP

    Plasmid

    #60659

    Purpose

    Labels synaptic vesicles for electron microscopy

    Depositor

    Scott Sternson

    Article

    Atasoy et al Nat Neurosci. 2014 Dec;17(12):1830-9. do

    Insert

    VAMP2:HRP
    

    Use

    AAV and Cre/Lox

    Tags

    Myc

    Promoter

    CAG

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pmCellFree_KA1 ZIKV E

    Plasmid

    #145341

    Purpose

    Cell-free/mammalian expression vector for Zika virus Envelope protein with N-term eGFP

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV Envelope
    

    Tags

    8xHis and eGFP

    Expression

    Bacterial

    Promoter

    T7- CMV/CAG

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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