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pET28a-MH6-uvsY

Plasmid

#163914

Purpose

Expresses uvsY for bacterial expression and affinity purification

Depositor

Paul Freemont

Publication

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsY (uvsY Enterobacteria phage T4)

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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VV063: 1xCox8 - superecliptic pHluorin in fck

Plasmid

#58500

Purpose

Expresses the pH sensor superecliptic pHluorin in the mitochondria of mammalian cells

Depositor

Adam Cohen

Publication

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - Superecliptic pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV_iPE-C_P2A_Csy4

Plasmid

#232428

Purpose

Expression plasmid for iPE-C prime editor RNPs with additional Csy4 protection

Depositor

Gil Westmeyer

Publication

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

iPE-C–P2A–Csy4

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PAX6 donor plasmid

Plasmid

#105239

Purpose

Knock in H2B-GFP into the human PAX6 locus

Depositor

Lorenz Studer

Publication

Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

Insert

PAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette

Use

TALEN

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p36-p37-p38-p40-pET-Duet-1

Plasmid

#175049

Purpose

Overexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coli

Depositor

Brian Kelch

Publication

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunits 2-5 (RFC4, RFC2, RFC3, RFC5 Human)

Expression

Bacterial

Promoter

T7 promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-EGFPKASH

Plasmid

#124882

Purpose

labelling of neuronal nuclear envelope with EGFP

Depositor

Vassilis Pachnis

Publication

Obata et al Nature. 2020 Feb;578(7794):284-289. doi:

Insert

EGFP

Use

AAV

Tags

KASH

Promoter

CaMKII

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mCherry-BRD4

Plasmid

#183939

Purpose

Transcriptional co-activator BRD4 labeled by mCherry

Depositor

Karsten Rippe

Publication

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

mCherry-BRD4 (Brd4 Mouse, Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pKD237-3a-2

Plasmid

#90957

Purpose

Expresses ThsR and mCherry constitutively and sfGFP under the PphsA promoter

Depositor

Jeffrey Tabor

Publication

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Thiosulfate response regulator
Superfolder GFP
mCherry

Expression

Bacterial

Promoter

Bba_J23100, Bba_J23105, and PphsA

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Histone H2B targeted Aurora B FRET sensor Kif2 substrate

Plasmid

#45216

Depositor

Michael Lampson

Publication

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

Histone H2B-targeted Aurora B FRET sensor

Expression

Mammalian

Promoter

T7

Available since

May 30, 2013

Availability

Academic Institutions and Nonprofits only

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pGLTR-X-GFP

Plasmid

#58245

Purpose

GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and eGFP

Depositor

Stephan Geley

Publication

Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

Inserts

attR1-Cm(R)-CcdB-attR2
TetR-NLS-T2A-GFP

Use

Lentiviral and RNAi; Gateway destionation vector

Tags

GFP, NLS, T2A, and TetR

Expression

Mammalian

Promoter

SFFV and no

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Inzolia library in pRDA_052

Pooled library

#209551

Purpose

The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs.

Depositor

John Doench

Publication

Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

Use

CRISPR and Lentiviral

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only
pTE4497

Plasmid

#80339

Purpose

mammalian expression FnCpf1 nuclease and Fn crRNA

Depositor

Ervin Welker

Publication

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Fn crRNA
FnCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV and human U6

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-C1 RPEL1-EGFP-3XNLS

Plasmid

#58469

Purpose

Expresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoter

Depositor

Dyche Mullins

Publication

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

MRTF-A RPEL1 (Mrtfa Mouse, Synthetic)
3X NLS with 3X stop codons

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCS2 GFP-GSK3mut-MAPK

Plasmid

#29690

Purpose

GFP reporter protein fused to three mutated GSK3 phosphorylation sites

Depositor

Edward De Robertis

Publication

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

GFP glycogen synthase kinase 3 recognition site mutated primed by MAPK

Tags

Flag and Flag-EGFP

Expression

Mammalian

Mutation

recognition sites only, and E3 ligase site, GSK3 …

Promoter

SP6

Available since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBAD-HisD-miniGFP1

Plasmid

#188896

Purpose

Express 6xHis-Tag miniGFP1 in E.coli

Depositor

Kiryl Piatkevich

Publication

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP1

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pC0037

Plasmid

#181934

Purpose

Dual secreted luciferase reporter (EF1alpha-Gaussia luciferase, CMV-Cypridina luciferase)

Depositor

Feng Zhang

Publication

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Inserts

Gaussia luciferase
Cypridina luciferase

Expression

Mammalian

Promoter

CMV and EF1alpha

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLH-sgRNA1

Plasmid

#75388

Purpose

sgRNA1

Depositor

Thoru Pederson

Publication

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-CAG-miniGFP1-P2A-FusionRed

Plasmid

#188732

Purpose

Express miniGFP1 and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP1

Use

AAV

Tags

FusionRed

Expression

Mammalian

Promoter

CAG

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pPPED

Plasmid

#162468

Purpose

Destination Expression vector for dicot plant prime editing

Depositor

Adam Bogdanove

Publication

Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

Inserts

Cas9H840A
attR gateway destination cassette

Tags

MLV reverse transcriptase

Expression

Plant

Mutation

Change histidine 840 to Alanine based sequence o…

Promoter

2x35S

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p2R3a-nosT

Plasmid

#71272

Purpose

Entry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Publication

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

nopaline synthase terminator

Use

Gateway Cloning

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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