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pET29_anti-SARS-CoV-2-NM1230

Plasmid

#209423

Purpose

Express anti-SARS-CoV-2-Nanobody NM1230 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-NM1230

Tags

Myc-6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-SARS-CoV-2-Nanobody-C5

Plasmid

#209420

Purpose

Express anti-SARS-CoV-2-Nanobody C5 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-Nanobody-C5

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-SARS-CoV-2-Nanobody-F2

Plasmid

#209421

Purpose

Express anti-SARS-CoV-2-Nanobody F2 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-Nanobody-F2

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLei-wt-sfGFP

Plasmid

#191113

Purpose

An E. coli sfGFP wild type control

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Tags

His tag

Expression

Bacterial

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-MfnG-TEV-His

Plasmid

#191114

Purpose

Expresses MfnG-TEV-His in E. coli

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

MfnG

Tags

His tag

Expression

Bacterial

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD

Plasmid

#226967

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD

Tags

His6 (on backbone)

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_LmFPPS

Plasmid

#186322

Purpose

pET-28a vector (Novagen) (encoding an N-terminal His tag), to transform Escherichia coli BL21 (DE3) cells

Depositor

Sandra Gabelli

Article

Aripirala et al Acta Crystallogr D Biol Crystallogr. 201

Insert

FPPS

Tags

His Tag

Expression

Bacterial

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJB53

Plasmid

#226309

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with V(T/S)G to AAA CsoS2 MR1-3

Expression

Bacterial

Mutation

VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

Promoter

pTRC

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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T. aq sigmaA

Plasmid

#215350

Purpose

Express T. aq sigmaA transcription initiation factor in E. coli cells

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

rpoD

Tags

6x His

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-TEV-AcrIC9

Plasmid

#207409

Purpose

Protein purification vector to produce AcrIC9 in E. coli

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

T7-TEV-AcrIC9

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-3C-AcrIC8

Plasmid

#207410

Purpose

Protein purification vector to produce AcrIC8 in E. coli

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

T7-HRV3C-AcrIC8

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-GTS-TurboID

Plasmid

#209397

Purpose

Transiently expressing TurboID fused with a Golgi targeting sequence (GTS) in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

GTS-3XHA-TurboID

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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1BR9-BFPSTOP

Plasmid

#202055

Purpose

E. coli expression vector for N-ter GFP11 R9 tagged BFP

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

BFP

Tags

6xHis and GFP11

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-L40A_AnaCNJKFGW_Bba-B0015

Plasmid

#202024

Purpose

Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant) in E. coli

Depositor

Mikhail Shapiro

Article

Hurt et al bioRxiv 2024.03.30.587094

Insert

Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant)

Expression

Bacterial

Mutation

GvpA T6A-L40A

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST17-HO1(51-231)

Plasmid

#203486

Purpose

For recombinant protein production in Escherichia coli. His-tagged Arabidopsis thaliana HEME OXYGENASE 1 (HO1) without the plastid transit peptide under the control of the T7 promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Arabidopsis thaliana HEME OXYGENASE1 (51 aa – 231 aa)

Expression

Bacterial and Plant

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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1BR9

Plasmid

#193862

Purpose

E. coli expression vector for N-ter GFP11 and C-ter R9 tagged proteins. Empty backbone for LIC cloning.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Type

Empty backbone

Tags

GFP11, Hisx6, and R9

Expression

Bacterial

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV W131G SPOP 1-374

Plasmid

#197019

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

W131G

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV E78K SPOP 1-374

Plasmid

#197018

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

E78K

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV E47K SPOP 1-374

Plasmid

#197017

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

E47K

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV W22R SPOP 1-374

Plasmid

#197016

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

W22R

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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pNIC-Bio2_MVPI_SMAC(delta(55))_9His

Plasmid

#197978

Purpose

E. coli expression of MVPI-SMAC(delta(55))_9His

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

Smac (DIABLO Human)

Tags

9His

Expression

Bacterial

Mutation

delta (1-55), A56M

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pNIC-Bio2_SMAC(delta(55))_Cys_9His

Plasmid

#197977

Purpose

E. coli expression of SMAC(delta(55))_Cys-9His

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

Smac (DIABLO Human)

Tags

9His

Expression

Bacterial

Mutation

delta (1-55), ins240C

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadRmt1)-DadR(ΔDBD)

Plasmid

#191637

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(ΔDBD) complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Expression

Bacterial

Mutation

ΔDBD

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadRmt2)-DadR(ΔTM)

Plasmid

#191638

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(ΔTM) complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Expression

Bacterial

Mutation

ΔTM

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadRmt4)-DadR(linker)

Plasmid

#191639

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(linker region shuffled) complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Expression

Bacterial

Mutation

Linker region sequence shuffled

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadR-NFLAG)-N-3xFLAG-DadR

Plasmid

#191640

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Tags

3xFLAG

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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mRuby3 only VLP

Plasmid

#182429

Purpose

YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and mRuby3 tagged with VP2C (GAL10 promoter). mRuby3 was codon-optimised for E. coli. Contains uracil marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al bioRxiv 2022.11.24.517869

Inserts

VP2C-mRuby3
deltaVP1

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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QUAS-0-T7-TetO-GFP_ColE1

Plasmid

#171662

Purpose

QUAS directly upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli. Contains the ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

QUAS-T7-TetO-GFP ssrA-T7 Stop

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET_His6-MBP-TEV-Cas6-RT-Cas1_WT

Plasmid

#186274

Purpose

Expresses Thiomicrospira Cas6-RT-Cas1 in E. coli

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicropira Cas6-RT-Cas1

Tags

His6-MBP

Expression

Bacterial

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-bhaA

Plasmid

#167810

Purpose

Expresses His6-tagged BhaA in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

BhaA

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-bhaB1

Plasmid

#167811

Purpose

Expresses His6-tagged BhaB1 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

BhaB1

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-bhaB7

Plasmid

#167813

Purpose

Expresses His6-tagged BhaB7 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

BhaB7

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b-bhaC1

Plasmid

#167814

Purpose

Expresses BhaC1 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

BhaC1

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S7-S83C/Δ157-178

Plasmid

#128592

Purpose

Expresses tagless S7-S83C, Δ157-178 mutant 30S ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

S7 E.coli ribosomal protein (rpsG E.coli)

Expression

Bacterial

Mutation

S83C, Δ157-178

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHBT-Mb(GDx-Clo_L10)

Plasmid

#183406

Purpose

Expresses Monobody GDx-Clo_L10 in E. coli

Depositor

Shohei Koide

Article

Kermani et al Nat Commun. 2020 Nov 27;11(1):6064. doi:

Insert

His tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)

Tags

His tag-Avi tag-TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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PdnaK-IR2-IR2 GFP pZa

Plasmid

#170083

Purpose

GFP expression under the control of E. coli dnaK promoter engineered with double IR2 HAIR motif from M. tuberculosis

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PdnaK-IR2-IR2

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PdnaK-IR3 GFP pZa

Plasmid

#170084

Purpose

GFP expression under the control of E. coli dnaK promoter engineered with IR3 HAIR motif from M. tuberculosis

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PdnaK-IR3

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PdnaK-IR2 GFP pZa

Plasmid

#170081

Purpose

GFP expression under the control of E. coli dnaK promoter engineered with IR2 HAIR motif from M. tuberculosis

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PdnaK-IR2

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-HisTim12_CtoA

Plasmid

#170281

Purpose

Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

Depositor

Paul Schanda

Article

Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

Insert

yeast Tim12 (TIM12 Budding Yeast)

Tags

TEV-cleavable N-terminal His tag (on backbone)

Expression

Bacterial

Mutation

mutated two non-conserved Cys to Ala

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-rDAD2 cPGFP(N180)

Plasmid

#166001

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rDAD2 cPGFP(N180)

Expression

Bacterial

Mutation

LSSmOrange- V49A (please see depositor comments)

Promoter

T7 promoter

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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