We narrowed to 21,976 results for: Sis

1. pAAV-FLEX-SaCas9-U6-sgGrin2a

   Plasmid

   #124850

   Purpose

   Mutagenesis of Grin2a

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin2a (Grin2a Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMDC32B-AtTAS1c-D2-B/c

   Plasmid

   #137884

   Purpose

   Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor into position downstream 3'D1[+]

   Depositor

   Alberto Carbonell

   Article

   Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

   Insert

   AtTAS1c-D2-B/c
   

   Use

   Tags

   Expression

   Plant

   Mutation

   A. thaliana TAS1c precursor sequence including a …

   Promoter

   2x35S

   Available since

   May 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. aMHC-Puro Rex-Blast

   Plasmid

   #21231

   Depositor

   Mark Mercola

   Article

   Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

   Insert

   alpha myosin heavy chain promoter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST_6xUAS:Lamp1-mGFP

   Plasmid

   #192356

   Purpose

   Lamp1 tagged with mGFP, expressed under the UAS promoter

   Depositor

   Francesca Peri

   Article

   Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

   Insert

   Lamp1 (LAMP1 Human)
   

   Use

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-HSF1Δ∼PRD

   Plasmid

   #71725

   Purpose

   plasmid expressing mic-his tag phosphorylation-deficient hHSF1

   Depositor

   Lea Sistonen

   Article

   Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

   Insert

   phosphorylation-deficient HSF1 (HSF1 Human)
   

   Use

   Tags

   mac 6xHIS

   Expression

   Mammalian

   Mutation

   15 phosphorylation sites in RD mutated to Alanine

   Promoter

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. hGATA-1 K137R

   Plasmid

   #118353

   Purpose

   This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hGATA-1 (GATA1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K137R

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPKm-248

   Plasmid

   #90504

   Purpose

   Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tPCYA - IRES - MTS - tHO1 - P2A - MTS - tFD - P2A - MTS - tFNR

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. hHSF1 K298R

   Plasmid

   #118362

   Purpose

   This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K298R

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PDZb-beta2(616-951)-mCherry

   Plasmid

   #100736

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP2B1 (AP2B1 Human)
   

   Use

   Tags

   PDZb and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICE-HA-CtIP-siR-WT

    Plasmid

    #82030

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    CtIP (RBBP8 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Mutations making the cDNA resistant to a siRNA

    Promoter

    CMV-tet

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTRE-Tight-FLAG-CYLD-C601A

    Plasmid

    #60029

    Purpose

    CYLD (C601A) catalytic dead under control of Tet promoter with N-terminal FLAG tag

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

    Insert

    Cylindromatosis (CYLD Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Cysteine 601 changed to Alanine

    Promoter

    TRE

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

12. GFP-CYLD-C601A

    Plasmid

    #60028

    Purpose

    N-terminal GFP tagged catalytic inactive (C601A) expression

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

    Insert

    Cylindromatosis (CYLD Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Cysteine 601 changed to Alanine

    Promoter

    CMV

    Available since

    Nov. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSUPER-hHSF2 shRNA

    Plasmid

    #118346

    Purpose

    This plasmid expresses shRNA against hHSF2.

    Depositor

    Lea Sistonen

    Article

    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

    Insert

    hHSF2 shRNA (HSF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR4 PARP2 E614Q

    Plasmid

    #192545

    Purpose

    pENTR plasmid Arabidopsis thaliana PARP2 CDS, E614Q mutation, no stop codon

    Depositor

    Lennart Wirthmueller

    Article

    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

    Insert

    poly(ADP-ribose) polymerase 2 E614Q (AT3G18250 Mustard Weed)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    changed Glu 614 to Gln

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR4 CDS A. thaliana PHOT1

    Plasmid

    #209187

    Purpose

    pENTR4 plasmid with CDS of Arabidopsis thaliana PHOTOTROPIN 1 (PHOT1, AT3G45780.1) without stop codon for C-terminal epitope tags

    Depositor

    Lennart Wirthmueller

    Article

    Simranjit et al bioRxiv 2023.09.25.558804

    Insert

    AT3G45780.1 (PHOT1 Mustard Weed)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Susd4(3.7)-EGFP-sv40

    Plasmid

    #184413

    Purpose

    AAV expression of tTA from mouse sushi domain containing 4 (Susd4) gene promoter.

    Depositor

    Marylka Yoe Uusisaari

    Article

    Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

    Insert

    Mouse Susd4 gene promoter-EGFP (Susd4 Mouse)
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Susd4 gene

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWZLblasti-HA-MEK1

    Plasmid

    #53161

    Purpose

    Expresses HA tagged human MEK1 from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pLVX-mCherry-SPASTIN, F124D

    Plasmid

    #180648

    Purpose

    Lentiviral expression vector for SPASTIN. Has F124D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-44.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Starts at M87, F124D mutation, has siRNA resistan…

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. His-DNPH1

    Plasmid

    #203224

    Purpose

    For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    DNPH1 (DNPH1 Human)
    

    Use

    Tags

    His tag (with TEV cleavage site)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF1α-M2rtTA-T2A-Puro

    Plasmid

    #169175

    Purpose

    piggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174

    Depositor

    Igor Slukvin

    Article

    Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:

    Insert

    M2rtTA-T2A-Puro
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    EF1a

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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