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HiBiT-FZD10

Plasmid

#195848

Purpose

Expresses human FZD10 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD10 (FZD10 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pYTRW26K_1Ti1

Plasmid

#177292

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and eYFP

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

PsacB-sacB

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSynapsin_NLS_GFP-P2A_SNAP_GluA2

Plasmid

#170455

Purpose

Expresses SNAP tagged GluA2 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

GluA2 (Gria2 Mouse)

Use

Lentiviral

Tags

SNAP

Expression

Mammalian

Promoter

Synapsin

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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HMGN1_pLENTI-CAG-IRES-GFP

Plasmid

#176995

Purpose

Mammalian lentiviral expression vector encoding HMGN1

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

HMGN1 (HMGN1 Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pUb-Cas9-mCherry_cU6:6-sgRNA

Plasmid

#190598

Purpose

The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter

Depositor

Claudia Rueckert

Article

Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

Inserts

Cas9
sgRNA

Use

CRISPR

Tags

mCherry - separated by T2A

Promoter

Aedes aegypti polyubiquitin and Culex quinquefasc…

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)

Plasmid

#224490

Purpose

Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Golgi-mTagBFP-P2A-H2A-iRFP

Use

Gateway Cloning

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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YA1531: pAAV_CAG-FLEX-QuasAr3

Plasmid

#107701

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

QuasAr3

Use

AAV

Expression

Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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px330-GAPDH

Plasmid

#136940

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4

Depositor

Jeremy Stark

Article

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting GAPDH (GAPDH Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD5

Plasmid

#195843

Purpose

Expresses human FZD5 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD5 (FZD5 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pME-lox-3xSTOP-lox (JDW 869)

Plasmid

#224521

Purpose

A Gateway compatible middle entry clone containing a loxP flanked SV40 polyA, bGH polyA, and SV40 pA stop cassette

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LoxP-3xStop-LoxP

Use

Cre/Lox

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

Plasmid

#52890

Purpose

"CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.

Depositor

Iswar Hariharan

Article

Bosch et al Development. 2015 Feb 1;142(3):597-606.

Inserts

LexGAD
Gal4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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YA1629: pAAV_CAG-FLEX-paQuasAr3-s

Plasmid

#107703

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

paQuasAr3

Use

AAV

Expression

Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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YA1627: pAAV_hSyn-Dio-paQuasAr3-s-P2A-CheRiff-s

Plasmid

#107704

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

paQuasAr3-P2A-CheRiff

Use

AAV

Expression

Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pETDuet-1 Flag-LplA

Plasmid

#232118

Purpose

For bacterial expression of Flag-tagged E. coli LplA.

Depositor

Ruth Sommese

Article

Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

Insert

lplA (lplA Escherichia coli (strain K12))

Tags

Flag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD9

Plasmid

#195847

Purpose

Expresses human FZD9 with an N-terminal HiBiT; contains P199R; I448V; S524N; R556H

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD9 (FZD9 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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Polymerase expression - pCMV-ePhi29 (LM2802)

Plasmid

#208967

Purpose

Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

ePhi29-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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mH2A2-CT-MYC

Plasmid

#45170

Depositor

Brian Chadwick, Hunt Willard

Article

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy2 (MACROH2A2, H2AFY2 Human)

Tags

HIS and MYC

Expression

Mammalian

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pDONR221-NUDT5

Plasmid

#219954

Purpose

For gateway cloning of NUDT5

Depositor

Karla Feijs

Article

Weixler et al Commun Biol. 2025 Mar 18;8(1):453. doi:

Insert

NUDT5 (NUDT5 Human)

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-eYFP-FKBP-MAD1-AA

Plasmid

#114038

Purpose

Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

Depositor

Geert Kops

Article

Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

Insert

Human MAD1 (MAD1L1 Human)

Tags

eYFP-FKBP12

Expression

Mammalian

Mutation

MAD2 binding deficient MAD1 K541A/L543A

Promoter

CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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H14-MBP-SUMO-Cys-Linker-BAF_G47E

Plasmid

#101779

Purpose

Expresses His-MBP-tagged mutant BAF (G47E) in E.coli (SUMO-cleavable)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Tags

His14-MBP-SUMO

Expression

Bacterial

Mutation

G47E mutation

Promoter

T5

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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