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pCS-H2B-mRFP1

Plasmid

#53745

Purpose

Contains a fusion of human histone H2B to monomeric red fluorescent protein 1 in the pCS2+ expression vector

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

mRFP

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP(C1)-PP1gamma

Plasmid

#44225

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Article

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Insert

PP-1G (PPP1CC Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Puro DEST (w16-1)

Plasmid

#17296

Purpose

3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning, Lentiviral, and RNAi

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pTALYM3B15

Plasmid

#47878

Purpose

TALE-mClover against mouse major satellite sequence

Depositor

Maria-Elena Torres-Padilla

Article

Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

Insert

TALE-mClover

Use

TALEN

Tags

mClover

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-3aHA

Plasmid

#18030

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3a (Wnt3a Mouse)

Use

Retroviral

Tags

HA

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-APOBEC1-YTHmut

Plasmid

#131637

Purpose

Expresses APOBEC1 fused to a mutant YTH domain with diminished m6A binding

Depositor

Kate Meyer

Article

Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

Insert

APOBEC1-YTHmut-HA (YTHDF2 Human, Rat)

Tags

HA

Expression

Mammalian

Mutation

YTH domain lacks AA 385-409 comprising the m6A-bi…

Promoter

CMV

Available Since

Oct. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCINeoFlag ALIX

Plasmid

#89859

Purpose

Expresses full length Flag-tagged ALIX in mammalian cells; internal ID WISP03-308

Depositor

Wesley Sundquist

Article

Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

Insert

ALIX (PDCD6IP Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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AA0250-mCherry-2a-hM4D-nrxn1a rev

Plasmid

#60544

Purpose

Co-expresses hM4Dnrxn and a red fluorescent label from a Cre-dependent virus

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Inserts

mCherry
muscarinic receptor 4, axon targeted variant (CHRM4 Human)

Use

AAV and Cre/Lox

Tags

2xHA

Promoter

CAG

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-RORA

Plasmid

#51303

Purpose

Expression of RORA after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

RAR-related orphan receptor A (RORA Human)

Use

Lentiviral

Tags

n/a

Mutation

full length ORF

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV4-p105

Plasmid

#23288

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

p105 (NFKB1 Human)

Expression

Mammalian

Available Since

April 29, 2010

Availability

Academic Institutions and Nonprofits only

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pLCA.66/2272

Plasmid

#22733

Depositor

Mark Magnuson

Article

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

Lox66-pU(delta)TK-EM7Neo-Lox2272

Use

Cre/Lox; Bac recombineering

Mutation

This vector was designed for assembling gene targ…

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TLR10-YFP

Plasmid

#13643

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 10 (TLR10 Human)

Tags

YFP

Expression

Mammalian

Available Since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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pPSU150

Plasmid

#173836

Purpose

Expresses 150 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABMBPpepN

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pEff

Plasmid

#97423

Purpose

Transient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.

Depositor

Nikolai Ravin

Article

Mardanova et al Front Plant Sci. 2017 Feb 28;8:247. doi:

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pMK344 (Hygro-P2A-mAID)

Plasmid

#121178

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLCK-mScarlet3_C1

Plasmid

#189771

Purpose

Construct for labelling plasma membranes in mammalian cells using LCK-mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

LCK-mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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NPY-mCherry

Plasmid

#67156

Purpose

Expresses NPY-mCherry

Depositor

Wolfhard Almers

Article

Barg et al Proc Natl Acad Sci U S A. 2010 Nov 30;10

Insert

NPYmCherry (NPY Human)

Tags

mCherry

Expression

Mammalian

Promoter

cmv

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-hSyn-eNpHR 3.0-EYFP

Plasmid

#26775

Purpose

3rd gen lentiviral expression of eNpHR 3.0 fused to EYFP driven by human Synapsin I promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Article

Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

Insert

eNpHR 3.0

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Trafficking Signal (TS) ER Export Signal

Promoter

hSyn

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2-UTY-F

Plasmid

#17439

Purpose

Expression of UTY in mammalian cells

Depositor

Kai Ge

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

Insert

UTY (UTY Human)

Tags

6xMyc and Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

Plasmid

#67979

Purpose

Cas9 activity reporter (control) with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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