We narrowed to 10,107 results for: transfer

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mOrange-P2A-hKvb1

Plasmid

#154084

Purpose

Independent expression of mOrange and human Kvbeta 1 under human synapsin 1 promoter

Depositor

Michael Hoppa

Article

Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

Insert

mOrange-P2A-human Kvb1 (KCNAB1 Synthetic, Human)

Use

AAV

Expression

Mammalian

Promoter

human synapsin 1

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shStk16-1

Plasmid

#180391

Purpose

Producing AAV that encodes mouse Stk16 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shStk16-1 (Stk16 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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SIN-cPPT-H1-sgHtt1-7SK-sgCas9-PGK-mCherry-WPRE

Plasmid

#87912

Purpose

Kamicas9 lentiviral vector

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

shHTT1, sgCas9, cherry

Use

Lentiviral

Promoter

H1, 7SK, PGK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV_Donor/Reporter-COL4A5

Plasmid

#130280

Purpose

COL4A5 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A5 donor template

Depositor

Silvestro Conticello

Article

Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

Insert

mCherry-eGFP

Use

AAV

Promoter

CMV

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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SIN-PGK-Cas9-WPRE

Plasmid

#87886

Purpose

To produce Lentiviral vector for gene editing

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

hCas9

Use

Lentiviral

Tags

nls

Mutation

Human codon-optimized

Promoter

mouse PGK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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p-AAV2-hSyn-MerMAID6-Citrine

Plasmid

#126521

Purpose

Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

Insert

MerMAID6

Use

AAV

Tags

Citrine

Promoter

human synapsin

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV::NLS-saCas9-NLS-3xHA-bGHpa;U6::BsaI-sgRNA-Grpr1

Plasmid

#175176

Purpose

A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Grpr

Depositor

Bernardo Sabatini

Article

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

sgRNA-Grpr

Use

AAV and CRISPR

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV2-TBG-FLAG-mRIPK1-S321A

Plasmid

#115342

Purpose

Liver-specific adeno-associated viral delivery and mammalian expression of Flag-mRIPK1-S321A

Depositor

Junying Yuan

Article

Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

Insert

RIPK1 (Ripk1 Mouse)

Use

AAV

Tags

Flag

Expression

Mammalian

Mutation

S321A

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS2272

Plasmid

#111898

Purpose

ssAAV genome with Ple342 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple342 (TUBB3 Human)

Use

AAV

Promoter

Ple342 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL

Plasmid

#172195

Purpose

AAV sequence-specific control vector for TALE-Tet1FL-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

Depositor

Garrett Kaas

Article

Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

Insert

HA-TALE(NG)-Tet1FL-NLS_2A_EGFP_W3SL

Use

AAV and Mouse Targeting; Tale

Tags

HA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

transcription regulatory domain removed

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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AAV_Dbh HMEJ donor

Plasmid

#97320

Purpose

HMEJ donor for fusing a p2A-mCherry-WPRE reporter to mouse Dbh. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Dbh HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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J8AAV-HDR Ctnnb1.1

Plasmid

#73452

Purpose

J8AAV-HDR U6sgCtnnb1.1 template

Depositor

Wen Xue

Article

Yin et al Nat Biotechnol. 2016 Feb 1. doi: 10.1038

Insert

Ctnnb1 (Ctnnb1 Mouse)

Use

AAV

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
pEF1a-CRY2PHR-SID4X

Plasmid

#63663

Purpose

AAV expression of CRYS2PHR-SID4X

Depositor

Dong Kong

Article

Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

Insert

SID4X-CYR2PHR

Use

AAV

Expression

Mammalian

Promoter

EF1alpha

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-EF1α1.1-FLEX-rc [SomArchon-GFP]

Plasmid

#153532

Purpose

AAV-mediated expression of SomArchon-GFP under the EF1α1.1 promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon-GFP

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Promoter

EF1α1.1

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV2-TBG-FLAG-mRIPK1-S321E

Plasmid

#115343

Purpose

Liver-specific adeno-associated viral delivery and mammalian expression of Flag-mRIPK1-S321E

Depositor

Junying Yuan

Article

Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

Insert

RIPK1 (Ripk1 Mouse)

Use

AAV

Tags

Flag

Expression

Mammalian

Mutation

S321E

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-APLP1ICD-IRES-hrGFP

Plasmid

#107546

Purpose

AAV-mediated expression of last 50 amino acids of APLP1 protein (APLP1ICD) and IRES-mediated co-expression of hrGFP to recognize labelled cells

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

APLP1ICD (APLP1 Human)

Use

AAV

Mutation

None

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.SF-iGluSnFR.S72A

Plasmid

#106188

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

CAG

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-iLMO (slGLuc-Mac-EYFP)

Plasmid

#114100

Purpose

fusion protein of Gaussia luciferase variant superluminescent, Leptosphaeria maculans opsin, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

Gaussia luciferase, superluminescent; Leptosphaeria maculans opsin; EYFP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-crRNA(TBK1)

Plasmid

#109322

Purpose

AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TBK1

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

mCherry-KASH (TBK1 Human)

Use

AAV

Promoter

hSyn

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-SEPT_Cdk2-T160E-siR

Plasmid

#73976

Purpose

HDR template to generate Cdk2-T160E-siR

Depositor

Steven Dowdy, Manuel Kaulich

Article

Kaulich et al Nucleic Acids Res. 2015 Apr 20;43(7):e45

Inserts

Cdk2-LHA-siR (CDK2 Human)
Cdk2-RHA-T160E (CDK2 Human)

Use

AAV

Mutation

T160 mutated to Glu and siRNA target site mutated

Available Since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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pscAAV- EF1α core -EGFP

Plasmid

#246994

Purpose

Can be used to generate AAV virus that will express EGFP from EF1α core promoter

Depositor

Ting Chen

Article

Chen et al Cell. 2025 Aug 14:S0092-8674(25)00857-8.

Insert

EGFP

Use

AAV

Promoter

EF1α

Available Since

April 10, 2026

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-hA53T

Plasmid

#253535

Purpose

overexpression of a human mutated form of aSynuclein (A53T)

Depositor

Won Do Heo

Article

Hyeon et al Nat Commun. 2025 Aug 21;16(1):7797. doi:

Insert

hSNCA (SNCA Human)

Use

AAV

Mutation

A53T

Promoter

hSyn1

Available Since

April 10, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV- EF1α core -NaChBac

Plasmid

#246997

Purpose

Can be used to generate AAV virus that will express NaChBac from EF1α core promoter

Depositor

Ting Chen

Article

Chen et al Cell. 2025 Aug 14:S0092-8674(25)00857-8.

Insert

NaChBac

Use

AAV

Promoter

EF1α

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-U6-gTgfbr2-CBh-mCherry-SV40late

Plasmid

#249125

Purpose

expression of gTgfbr2 and mCherry

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gTgfbr2 (Tgfbr2 Mouse)

Use

AAV

Promoter

hU6

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-U6-gThy1-CBh-mCherry-SV40late

Plasmid

#249126

Purpose

expression of gThy1 and mCherry

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gThy1 (Thy1 Mouse)

Use

AAV

Promoter

hU6

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-U6-gTnfrfs1a-CBh-mCherry-SV40late

Plasmid

#249127

Purpose

expression of gTnfrsf1a and mCherry

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gTnfrsf1a (Tnfrsf1a Mouse)

Use

AAV

Promoter

hU6

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-KIKO-U6-gOsmr-p2a-mCherry-STOP-pA-400

Plasmid

#249130

Purpose

all-in-one HDRT-based knockin-knockout (KIKO) design to insert mCherry (=knockin) at gOsmr cut site, disrupting Osmr expression

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gOsmr, mCherry (Osmr Mouse, Synthetic)

Use

AAV

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-U6-gCtnnb1-CBh-mCherry-SV40late

Plasmid

#249119

Purpose

expression of gCtnnb1 and mCherry

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gCtnnb1 (Ctnnb1 Mouse)

Use

AAV

Promoter

hU6

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE3G-CytoTape-FLAG

Plasmid

#252514

Purpose

Timestamp monomer based on CytoTape for encoding time along protein tape recorder for mouse brain in vivo applications

Depositor

Changyang Linghu

Article

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

CytoTape-FLAG

Use

AAV

Expression

Mammalian

Promoter

TRE3G

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIα-EGFP-CRY2-STIM1(318-450)

Plasmid

#248300

Purpose

An AAV construct designed to express a CRY2-fused truncated STIM1 fragment (a.a.318–450) under the control of the CaMKIIα promoter.

Depositor

Sangkyu Lee

Article

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

CRY2-fused truncated STIM1 fragment (a.a.318–450) (STIM1 Human)

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-317,451-685

Promoter

CamKlla

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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