We narrowed to 35,837 results for: CaS

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PET-B-Sniper SpCas9-NLS-6xHis

Plasmid

#207377

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-Sniper SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

F539S, M763I, K890N, amino acids 1005-1013 replac…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgDrd1

Plasmid

#159917

Purpose

Mutagenesis of Drd1

Depositor

Larry Zweifel

Insert

Drd1 (Drd1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pattB-LSL-AAVR-F2A-spCas9

Plasmid

#202459

Purpose

The plasmid backbone used to generate the recombination template to generate the SELECTIV mice through Integrase Mediated Transgenesis. Allows for Cre-dependent expression of AAVR and Cas9.

Depositor

Jan Carette

Article

Zengel et al Nat Methods. 2023 Jul;20(7):1070-1081. d

Insert

AAVR (AU040320 Mouse)

Use

CRISPR, Cre/Lox, and Mouse Targeting

Tags

mCherry fusion

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPS22 (pET23a-Cas10/Csm/csm2H6N)

Plasmid

#70038

Purpose

Bacterial expression of the S. epidermidis Cas10·Csm complex

Depositor

Luciano Marraffini

Article

Hatoum-Aslan et al J Biol Chem. 2013 Sep 27;288(39):27888-9

Insert

Cas10/Csm complex

Tags

6xHIS on N terminus of Csm2

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(SapI)-EFS-CasRx-pA

Plasmid

#192488

Purpose

To express CasRX and a gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pC0045-EF1a-PguCas13b-NES-HIV

Plasmid

#103861

Purpose

Expresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

PguCas13b

Use

CRISPR and Lentiviral

Tags

3xHA and HIV NES

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-BLST-dCas9-VPR

Plasmid

#115148

Purpose

Safe harbor site 231 knock-in vector with BlastR-dCas9-VPR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

BlastR-P2A-dCas9-VPR

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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FUGW U6 gL1HSg2dCas9-KRAB-T2a-GFP

Plasmid

#234881

Purpose

L1HS-silencing plasmid (CRISPRi gRNA2)

Depositor

Johan Jakobsson

Article

Adami et al bioRxiv 2025.01.17.633315

Insert

LacZ gRNA

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW U6 gL1HSg1dCas9-KRAB-T2a-GFP

Plasmid

#234882

Purpose

L1HS-silencing plasmid (CRISPRi gRNA1)

Depositor

Johan Jakobsson

Article

Adami et al bioRxiv 2025.01.17.633315

Insert

L1HS-silencing plasmid (CRISPRi gRNA1)

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcs2+spCas9 ABE-Umax-nanos

Plasmid

#222142

Purpose

Adenine base editor with modified TadA variant for germline A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9 with nanos UTR

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

SP6

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XGFP

Plasmid

#64111

Purpose

Expression of St1 dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pLGR002 LGR Cas9 guide vector

Plasmid

#188320

Purpose

Universal backbone for CRISPR/Cas9 sgRNA expression

Depositor

Laralynne Przybyla

Article

Heo et al Genome Biol. 2024 Jan 19;25(1):25. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

PuroR-T2A-mTagBFP2

Expression

Mammalian

Promoter

pU6 5'CMV and EF1-alpha

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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Px330-EGFP-LRRK2-CRISPR/Cas9

Plasmid

#180437

Purpose

Plasmid to express Cas9 from S.pyogenesand CRISPR gRNA to introduce LRRK2-G2019S mutation in human cells

Depositor

Frank Soldner

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

gRNA targeting LRRK2-G2019S (LRRK2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

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PET-Sniper SpCas9-NLS-6xHis

Plasmid

#207374

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Sniper SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

F539S, M763I, K890N

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pTRE3G-VP64-dCas9-VP64-ZsG

Plasmid

#192668

Purpose

Dox-inducible expression of VP64-dCas9-VP64 with 2A ZsGreen1

Depositor

Jeffrey Miner

Insert

VP64-dCas9-VP64, ZsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

TRE3G

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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LLP457 pGK-dCas9-Suntag-BFP

Plasmid

#100957

Purpose

BPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tag

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, SunTag array (10xGCN4)

Use

CRISPR

Tags

3xHA and BFP

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

Plasmid

#199252

Purpose

Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

High specificity eSpCas9(1.1)-D10A nickase

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A K848A K1003A R1060A

Promoter

CAG promoter

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pWalium20-10XUAS-3XFLAG-dCas9-VPR

Plasmid

#78897

Purpose

Expresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for in vivo CRISPRa

Depositor

Norbert Perrimon

Article

Lin et al Genetics. 2015 Oct;201(2):433-42. doi: 1

Insert

dCas9-VPR

Tags

3xFLAG

Expression

Insect

Promoter

10XUAS

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SauriCas9-KKH-Puro

Plasmid

#135966

Purpose

Expresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pTRE-Tight caspase-3 (p12)::nz [TU#817]

Plasmid

#16084

Depositor

Martin Chalfie

Article

Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

Insert

caspase-3 (p12) :: nz (CASP3 Human)

Expression

Mammalian

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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