We narrowed to 13,845 results for: Cas9

1. M-SPcas

   Plasmid

   #48668

   Purpose

   Mammalian S. pyogenes Cas9 expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. DS-ST1casN-

   Plasmid

   #48659

   Purpose

   Bacterial nuclease-null ST1 Cas9 expression

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9, nuclease-null
   ST1 tracrRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D to A, DH to AA, N to A

   Promoter

   proC

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. DS-ST1cas

   Plasmid

   #48647

   Purpose

   Bacterial S. thermophilus #1 Cas9 (ST1) + tracrRNA expression, cloDF13/spectinomycin

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9
   ST1 tracrRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   proC

   Available since

   Nov. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMB67

   Plasmid

   #47947

   Purpose

   Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and egl-13 NLS

   Expression

   Worm

   Mutation

   Promoter

   hsp-16.48

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. DS-TDcasN-

   Plasmid

   #48660

   Purpose

   Bacterial nuclease-null TD Cas9 expression

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9, nuclease-null
   TD tracrRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D to A, DH to AA, N to A

   Promoter

   proC and tracrRNA promoter

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSHS293

   Plasmid

   #87348

   Purpose

   D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Use

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/D435C/C574S/E945C

   Promoter

   T7

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. BCJJ344

   Plasmid

   #117503

   Purpose

   UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtUBI10

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSHS248

   Plasmid

   #87354

   Purpose

   S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Use

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/C574S/S867C/N1054C

   Promoter

   T7

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMLS544

   Plasmid

   #188882

   Purpose

   Integrating Pmex-5::Cas9 expression vector

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   Pmex-5::Cas9
   

   Use

   Tags

   Expression

   Worm

   Mutation

   C. elegans codom optimization and intron addition

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMLS546

    Plasmid

    #188883

    Purpose

    Integrating Pmex-5::Cas9 expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Pmex-5::Cas9
    

    Use

    Tags

    Expression

    Worm

    Mutation

    C. elegans codom optimization

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. dSp-VP64-RTA

    Plasmid

    #99675

    Purpose

    Expresses dSp Cas9 fused at C term. To VP64 and RTA activation domains

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    Tags

    VP64-RTA

    Expression

    Mammalian

    Mutation

    dead Cas9

    Promoter

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. BCJJ340

    Plasmid

    #117502

    Purpose

    UBI10:Cas9:E9 Golden Gate Level 1 Position 2

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBI10

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Cas-Acr (insertion 5)

    Plasmid

    #128116

    Purpose

    Expresses SpyCas9 fused to attenuated AcrIIA4 (insertion 5)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    CascAID (insertion 5)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Cas-Acr (N39A)

    Plasmid

    #128117

    Purpose

    Expresses SpyCas9 fused to attenuated AcrIIA4 (N39A)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    CascAID (N39A)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBBK31 pHomL-TDH3p-Scarlet(rfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

    Plasmid

    #179015

    Purpose

    Parent vector for markerless yeast integration cassettes with N-terminal Scarlet(rfp)-tagged genes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cas-Acr (wtAcrIIA4)

    Plasmid

    #128115

    Purpose

    Expresses SpyCas9 fused to wild-type AcrIIA4

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    CascAID (wtAcrIIA4)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Cas-Acr (D14A/G38A)

    Plasmid

    #128118

    Purpose

    Expresses SpyCas9 fused to attenuated AcrIIA4 (D14A/G38A)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    CascAID (D14A/G38A)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. dSp-Rta 125-190

    Plasmid

    #99664

    Purpose

    Expresses dSp Cas9 fused to truncated RTA activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    Tags

    truncated RTA activation domain ( 125-190)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. dSp-Rta 125-175

    Plasmid

    #99668

    Purpose

    Expresses dSp Cas9 fused to truncated RTA activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    Tags

    truncated RTA activation domain (125-175)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Promoter

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. dSp-VP64-RTA(75-190)

    Plasmid

    #99669

    Purpose

    Expresses dSp Cas9 fused to combination of truncated activation domains

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    Tags

    VP64-RTA(75-190)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Promoter

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge