We narrowed to 19,001 results for: Tec

1. pU6Rosa-CAG-Cas9

   Plasmid

   #118998

   Purpose

   Mammalian expression of Rosa26 sgRNA, Cas9 and BFP

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   Cas9
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. V957 pLPS-3' ECFP-mito - Acceptor

   Plasmid

   #11699

   Depositor

   Tony Pawson

   Article

   Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

   Type

   Empty backbone

   Use

   Cre/Lox; Acceptor vector

   Tags

   ECFP-mito

   Expression

   Mammalian

   Available Since

   May 26, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pBT362

   Plasmid

   #127844

   Purpose

   clone BE4max-NG CBEs by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   BE4max-NG without deaminase
   

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHCKan-yibDp-GST

   Plasmid

   #134592

   Purpose

   Low Phosphate inducible GST

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   Glutathione S-transferase
   

   Tags

   none

   Expression

   Bacterial

   Promoter

   yibD

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. BCJJ340

   Plasmid

   #117502

   Purpose

   UBI10:Cas9:E9 Golden Gate Level 1 Position 2

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtUBI10

   Available Since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330A_FokI-1x4

   Plasmid

   #63604

   Purpose

   Expresses FokI-dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   FokI-dCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. TAL2368

   Plasmid

   #36779

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   NCOR1-TALEN_Left (Ncor1 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

8. mApple-VASP-5

   Plasmid

   #54958

   Purpose

   Localization: Actin/Focal Adhesions, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   VASP
   

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   A209T in VASP

   Promoter

   CMV

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pCellFree_G03 SREBF2

   Plasmid

   #67129

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   SREBF2 (SREBF2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pTMS2

    Plasmid

    #87800

    Purpose

    For constructing MBP-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.

    Depositor

    Yu-Zhu Zhang

    Article

    Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

    Type

    Empty backbone

    Tags

    6xHis and Strep II

    Expression

    Bacterial

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB5935

    Plasmid

    #106128

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntA_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCellFree_G03 YY1

    Plasmid

    #67082

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    YY1 (YY1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pLKO.1P shMOCS3_2

    Plasmid

    #102974

    Purpose

    Suppression of MOCS3

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shMOCS3_2 (MOCS3 Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1P shMOCS3_1

    Plasmid

    #102973

    Purpose

    Suppression of MOCS3

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shMOCS3_1 (MOCS3 Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. YSBE 628 : pHYG_mCherry

    Plasmid

    #92092

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; mCherry tag, HYG selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    mCherry
    

    Use

    tagging proteins in cryptococcus neoforman

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB2192

    Plasmid

    #67539

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Expression

    Yeast

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC57-8xHis

    Plasmid

    #101699

    Purpose

    8xHis in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    AATG-[8xHis]-AGCC
    

    Use

    Yeast expression

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. YSBE 558 : pNAT_4xFLAG

    Plasmid

    #92086

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, NAT selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    4xFlag
    

    Use

    tagging proteins in cryptococcus neoforman

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pML2003

    Plasmid

    #193255

    Purpose

    Propionate sensor expressing eYFP reporter

    Depositor

    Lauren Andrews

    Article

    Lebovich et al Front Bioeng Biotechnol. 2022 Aug 25;10:

    Inserts

    prpR
    eYFP
    

    Use

    Synthetic Biology

    Promoter

    Pprp and Synthetic promoter P6

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA4/TO-2xStrep-BFLF1

    Plasmid

    #162626

    Purpose

    Expresses N-terminal 2x-Strep-tagged BFLF1 in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    BFLF1
    

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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