We narrowed to 166,601 results for: Gene

1. pFAB3677

   Plasmid

   #47833

   Purpose

   BIOFAB RFP reporter plasmid for measuring promoter 2 + BCD1 efficiency.

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   promoter 2 and BCD1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   see insert

   Available Since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pINDgw RFA-V5-EcoDam

   Plasmid

   #59204

   Purpose

   Mammalian DamID vector for fusing gene of interest with Dam-V5 using Gateway cloning

   Depositor

   Bas van Steensel

   Article

   Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

   Type

   Empty backbone

   Use

   Damid

   Tags

   Dam (DNA adenine methyltransferase) and V5

   Expression

   Mammalian

   Promoter

   Heat Shock Minimal Promoter

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFAB3833

   Plasmid

   #47839

   Purpose

   BIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   promoter 8 and BCD1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   see insert

   Available Since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. MPRA_minCMV+GFP

   Plasmid

   #204866

   Purpose

   Vector containing GFP driven by minimal CMV promoter. This cassette can be cloned into MPRA vectors to serve as a reporter gene.

   Depositor

   Philipp Maass

   Article

   Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

   Insert

   minCMV:GFP
   

   Expression

   Bacterial and Mammalian

   Promoter

   minimal CMV

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFAB3737

   Plasmid

   #47838

   Purpose

   BIOFAB RFP reporter plasmid for measuring promoter 7 + BCD1 efficiency.

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   promoter 7 and BCD1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   see insert

   Available Since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. E12-cTAP

   Plasmid

   #20916

   Depositor

   Stephen Tapscott

   Article

   Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

   Insert

   E12 cTAP (Tcf3 Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   TAP tag is fused to the 3' end of the E12 ge…

   Available Since

   May 15, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pCfB3034(X-3 MarkerFree)

   Plasmid

   #73272

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-Zeo-TTF-1

   Plasmid

   #75089

   Purpose

   retrovirus-mediated gene transfer into mammalian cells

   Depositor

   David Mu

   Article

   Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

   Insert

   TTF-1 (NKX2-1 Human)
   

   Use

   Retroviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   wild-type, full-length, without native 3' UTR

   Available Since

   May 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pL90-Nat-2xHO

   Plasmid

   #139069

   Purpose

   CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

   Depositor

   Gianni Liti

   Article

   Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

   Insert

   Two guide RNAs targeting the HO endonuclease
   

   Expression

   Bacterial and Yeast

   Promoter

   TDH3

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. natMX-ins7

    Plasmid

    #195044

    Purpose

    pFA6a derived selection cassette 5' flanked with tCYC1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    NatR
    

    Use

    Yeast genomic targeting

    Tags

    tCYC1 S. cerevisiae, pPGK1 C. glabrata, NatR, tPG…

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFAB3689

    Plasmid

    #47834

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 3 + BCD1 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 3 and BCD1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    see insert

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. FASTHDR-Cterm-NanoLuc-Puromycin

    Plasmid

    #167209

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    NanoLuc

    Expression

    Mammalian

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PAX6 sgRNA7

    Plasmid

    #68466

    Purpose

    targeting PAX6 gene

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    sgRNA targeting PAX6 (PAX6 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDN-G1Gh

    Plasmid

    #44507

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
    yEGFP
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCfB2899(X-2 MarkerFree)

    Plasmid

    #73271

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMDC32B-OsMIR390-B/c

    Plasmid

    #61467

    Purpose

    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

    Depositor

    James Carrington

    Article

    Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

    Insert

    OsMIR390-B/c
    

    Expression

    Plant

    Mutation

    O. sativa MIR390 precursor sequence including a c…

    Promoter

    2x35S

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB2903(XI-2 MarkerFree)

    Plasmid

    #73275

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAcrF2

    Plasmid

    #89234

    Purpose

    Plasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage site

    Depositor

    Blake Wiedenheft

    Article

    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

    Insert

    AcrF2
    

    Tags

    6xHis-TEV

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfB3039(XII-2 MarkerFree)

    Plasmid

    #73279

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-2 (Chr XII: 808805..809939)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMDC123SB-OsMIR390-B/c

    Plasmid

    #61466

    Purpose

    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

    Depositor

    James Carrington

    Article

    Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

    Insert

    OsMIR390-B/c
    

    Expression

    Plant

    Mutation

    O. sativa MIR390 precursor sequence including a c…

    Promoter

    2x35S

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge