We narrowed to 27,720 results for: Spr

1. pSimpleII-NmCas9-FLAG

   Plasmid

   #47872

   Purpose

   FLAG tagged NmCas9 with no NLS.

   Depositor

   Erik Sontheimer, James Thomson

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Insert

   NmCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. px330-USP13

   Plasmid

   #61812

   Purpose

   to make USP13 Knockout cell line

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   USP13 (USP13 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pET15-MTFmt

   Plasmid

   #31174

   Depositor

   Linda Spremulli

   Article

   Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

   Insert

   Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)
   

   Use

   Tags

   His and thrombin cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pAN-PA2-RFP

   Plasmid

   #62248

   Purpose

   reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   mRFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pA2

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMM731

   Plasmid

   #68896

   Purpose

   IPTG-inducible CRISPRi vector targeting NanoLuc (NL1), constiutive NanoLuc, pNBU2 backbone, AmpR

   Depositor

   Timothy Lu, Christopher Voigt

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Inserts

   dCas9
   LacI
   sgRNA
   NanoLuc
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMM763

   Plasmid

   #68900

   Purpose

   IPTG-inducible CRISPRi vector targeting PcfiA (PR1), constiutive NanoLuc, pNBU2 backbone, AmpR

   Depositor

   Timothy Lu, Christopher Voigt

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Inserts

   dCas9
   LacI
   sgRNA
   NanoLuc
   

   Use

   Tags

   Expression

   Mutation

   G1104E

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAN-PA1-RFP

   Plasmid

   #62247

   Purpose

   reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   mRFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pA1

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAN-PTet-YFP

   Plasmid

   #62245

   Purpose

   used to characterize the promoter activities of PTet for dCas9 and sgRNA response functions.

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EE-SP!gIII

   Plasmid

   #48667

   Purpose

   Bacterial SP Cas9 targeting filamentous phage gene III at five protospacers

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9
   anti-gIII tracrRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   proC and tracrRNA promoter

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAN-PA3-RFP

    Plasmid

    #62249

    Purpose

    reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    mRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pA3

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMM725

    Plasmid

    #68895

    Purpose

    IPTG-inducible CRISPRi vector targeting NanoLuc (NL3), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pvTRAP

    Plasmid

    #50047

    Purpose

    expresses plasmodium vivax TRAP adhesion domains (25-283) in mammalian cells

    Depositor

    Timothy Springer

    Article

    Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

    Insert

    plasmodium vivax TRAP
    

    Use

    Tags

    6Xhis tag

    Expression

    Mammalian

    Mutation

    S42Q, N91S, N128S, S180R

    Promoter

    cmv

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pMM750

    Plasmid

    #68899

    Purpose

    IPTG-inducible CRISPRi vector targeting PcfiA (PR2), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAN-PBAD-YFP

    Plasmid

    #62246

    Purpose

    used to characterize the promoter activities of PBAD for dCas9 and sgRNA response functions.

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMM732

    Plasmid

    #68897

    Purpose

    IPTG-inducible CRISPRi vector targeting NanoLuc (NL2), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. B33

    Plasmid

    #74008

    Purpose

    gRNA for B108

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    none

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. B51

    Plasmid

    #74005

    Purpose

    gRNA for B108

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    none

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. B53

    Plasmid

    #74006

    Purpose

    gRNA for B108

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    none

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. B31

    Plasmid

    #74007

    Purpose

    gRNA for B108

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    none

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAN-PA5-RFP

    Plasmid

    #62251

    Purpose

    reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    mRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pA5

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge