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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 59,138 results for: lat

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Showing: 3681 - 3700 of 59138 results

  1. pEF1a-hMLH1NTD-NLS

    Plasmid
    #174826
    Purpose
    Mammalian expression of human MLH1NTD-NLS (codon optimized)
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human MLH1_NTD-NLS (codon optimized)
    Tags
    SV40 bpNLS
    Expression
    Mammalian
    Mutation
    truncation of residues 1-335
    Promoter
    EF1a
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174826-plasmid-map-sequence-id-343633

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  3. GFP-UBR5 ∆HECT

    Plasmid
    #52051
    Purpose
    CMV driven mammalian expression of ligase-dead UBR5 with an N-termianl GFP fusion
    Depositor
    Darren Saunders
    Publication
    Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    UBR5 ∆HECT (UBR5 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    C2769A
    Promoter
    CMV
    Available since
    March 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52051-plasmid-map-sequence-id-80161

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  6. pGL3-Basic-ACSL3-sfGFP(C) HDR template

    Plasmid
    #129413
    Purpose
    HDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFP
    Depositor
    Elina Ikonen
    Publication
    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ACSL3 HDR template (ACSL3 Human)
    Use
    CRISPR and TALEN; Endogenous tagging hdr template
    Tags
    sfGFP
    Expression
    Mammalian
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129413-plasmid-map-sequence-id-254784

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  7. pcDNA3.1+ eiF4A1 myc HIS

    Plasmid
    #71657
    Purpose
    mammalian expression of eiF4A1
    Depositor
    Gideon Dreyfuss
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eiF4A1 (EIF4A1 Human)
    Tags
    HIS and myc
    Expression
    Mammalian
    Mutation
    aa403 from Ala to Arg
    Promoter
    CMV
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71657-plasmid-map-sequence-id-422687

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  8. CaV1.3e[8a,11,31b,Δ32,42a]

    Plasmid
    #49333
    Purpose
    Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].
    Depositor
    Diane Lipscombe
    Publication
    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CACNA1D (Cacna1d Rat)
    Expression
    Mammalian
    Mutation
    Contains exons 8a, 11, 31b and 42a.Deletion of ex…
    Promoter
    CMV
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    49333-plasmid-map-sequence-id-159439

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  13. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139090-plasmid-map-sequence-id-273886

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  14. Vimentin - APEX2 in pECFP

    Plasmid
    #66170
    Purpose
    APEX2 fused to the C-terminus human vimentin
    Depositor
    Alice Ting
    Publication
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human Vimentin - APEX2 (VIM Human, Synthetic)
    Tags
    Flag epitope tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    APEX2 contains 4 mutations relative to wt soybean…
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    66170-plasmid-map-sequence-id-113682

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  16. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

    Plasmid
    #73440
    Purpose
    Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE-P4A6-vioC-P3A2-vioD
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PG6, P4A6, and P3A2 (orthogonal T7-lac variants)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73440-plasmid-map-sequence-id-135339

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Showing: 3681 - 3700 of 59138 results