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p415TEF cyto roGFP(iL)-Grx1(human)

Plasmid

#65002

Purpose

Yeast expression plasmid for roGFP1(iL)-Grx1; yeast codon optimized roGFP; human Grx1

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

Insert

Glutaredoxin-1 (GLRX Human)

Tags

roGFP(iL)

Expression

Yeast

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pQTEV-WDR41

Plasmid

#34808

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

WD repeat domain 41 (WDR41 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pGB-REL

Plasmid

#27277

Depositor

Thomas Gilmore

Article

Leeman et al Oncogene. 2008 Dec 4. 27(53):6770-81.

Insert

REL (REL Human)

Tags

GAL4 (aa1-147)

Expression

Yeast

Available Since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only
7093 pNOTAG Hsp104 T317A

Plasmid

#1242

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Hsp104 (T317A) (HSP104 Budding Yeast)

Expression

Bacterial

Mutation

T317A

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pT7RNAP-Q737P

Plasmid

#175356

Purpose

Support plasmid for T7_ori plasmids

Depositor

Sven Panke

Article

Becker et al Nucleic Acids Res. 2021 Jul 13. pii: 632

Insert

T7RNAP-Q737P

Expression

Bacterial

Mutation

Q737P

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pTM-NiV-L

Plasmid

#234657

Purpose

Expresses L gene of NiV Bangladesh strain

Depositor

Carolina Lopez

Article

Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

Insert

NiV L gene (L Nipah virus)

Use

In cell lines expressing bacteriophage t7 rna pol…

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTM-NiV-N

Plasmid

#234655

Purpose

Expresses N gene of NiV Bangladesh strain

Depositor

Carolina Lopez

Article

Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

Insert

NiV N gene (N Nipah virus)

Use

In cell lines expressing bacteriophage t7 rna pol…

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pFGAMS-mMaple3

Plasmid

#154912

Purpose

Expresses human PFAS-mMaple3 fusion in mammalian cells

Depositor

Stephen Benkovic

Article

Chan et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

PFAS (PFAS Human)

Tags

mMaple3

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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Plas-gRNA-LEU2

Plasmid

#157656

Purpose

GFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequence

Depositor

Frank Albert

Article

Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

Insert

GFP-gRNA

Use

Insert storage (replicative in e. coli)

Tags

GFP-gRNA

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051-sgMLH1-1

Plasmid

#125776

Purpose

constitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgMLH1-1 (MLH1 Human)

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051-sgMLH1-2

Plasmid

#125777

Purpose

constitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgMLH1-2 (MLH1 Human)

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-intein/Src

Plasmid

#71406

Purpose

Expresses Src inserted with a Npu DnaE intein (F1 construct)

Depositor

Huiwang Ai

Article

Ren et al J Am Chem Soc. 2015 Feb 18;137(6):2155-8

Insert

Src active kinase domain inserted with Npu DnaE intein

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only
pQTEV-AK1

Plasmid

#31290

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

adenylate kinase 1 (AK1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pGMCKq1-0X1-sspBmyc

Plasmid

#27068

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

PmyctetO-sspB (sspB synthetic gene)

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pTM-NiV-P

Plasmid

#234656

Purpose

Expresses P gene of NiV Bangladesh strain

Depositor

Carolina Lopez

Article

Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

Insert

NiV P gene (Nvgp2 Nipah virus)

Use

In cell lines expressing bacteriophage t7 rna pol…

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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Cav2.2 pcDNA3

Plasmid

#206072

Purpose

expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRSITEP-puro-shWRN1-C911

Plasmid

#125784

Purpose

a seed-matched control for pRSITEP-puro-shWRN1

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN1-C911 (WRN Human)

Use

RNAi

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCLC3sFlag

Plasmid

#52422

Purpose

mammalian expression of flag tagged rat CLC3 short transcript

Depositor

Steven Weinman

Article

Shimada et al Am J Physiol Gastrointest Liver Physiol.

Insert

CLC3 (Clcn3 Rat)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pQTEV-KCNIP2

Plasmid

#34799

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

Kv channel interacting protein 2 (KCNIP2 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pmU1-CasRx-non-targeting (pKW83)

Plasmid

#229051

Purpose

Cloning backbone for CasRx crRNA (Mouse U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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