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pPROK AdoHcyase

Plasmid

#14683

Depositor

Michael Hershfield

Article

Coulter-Karis et al Ann Hum Genet. 1989 May . 53(Pt 2):169-7

Insert

adenosyl-homocysteine hydrolase (AHCY Human)

Expression

Bacterial

Available Since

May 1, 2007

Availability

Academic Institutions and Nonprofits only
pLux01

Plasmid

#78281

Purpose

mRFP1 expressed behind an AHL-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

AHL inducible mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pXP418

Plasmid

#26843

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pDonor14-N7HELIX-KanR

Plasmid

#190663

Purpose

Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Promoter

NA

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR221-NR1H3

Plasmid

#79514

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

LXR Alpha (NR1H3 Human)

Use

Gateway Cloning

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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883 pET15b PTEN

Plasmid

#10936

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Tags

His

Expression

Bacterial

Available Since

Jan. 3, 2006

Availability

Academic Institutions and Nonprofits only
pPAiRFP1-N1

Plasmid

#44272

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Nat Commun. 2013 Jul 10;4:2153. doi: 10.

Insert

PAiRFP1

Expression

Mammalian

Mutation

G127D/S141R/M163L/Q168L/A203V/G218S/R220P/V244F/A…

Promoter

CMV

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pmCherry-PH(Akt2)

Plasmid

#86596

Purpose

Expresses mCherry-tagged PH(WT) domain of human Akt2

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2 PH domain (AKT2 Human)

Expression

Mammalian

Mutation

Amino acids 1 - 108

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-cyto-iATPSnFR1.0

Plasmid

#102553

Purpose

expresses cytoplasmic ATP sensor in astrocytes

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Use

AAV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Connexin43-GFP-APEX

Plasmid

#44440

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

Insert

Connexin43 -EGFP - pea APEX

Expression

Mammalian

Mutation

K14D, W41F, E112K mutations on APEX

Promoter

CMV

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pTALEN_v2 (NN)

Plasmid

#32191

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Nuclease, NN for 0.5 repeat

Expression

Mammalian

Available Since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pLL7.0: mTiam1(64-437)-tgRFPt-SSPB R73Q

Plasmid

#60418

Purpose

Mammalian Expression of Tiam DH/PH-tgRFPt-Micro

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

mTiam1-tgRFPt-SSPB R73Q

Use

Cre/Lox and Lentiviral

Tags

tgRFPt

Expression

Mammalian

Mutation

mTiam 64-437 // SSPB R73Q, Y11K and A15E

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD IFP1.4

Plasmid

#91775

Purpose

Bacterial expression of a fluorescent protein, Infrared Fluorescent Protein 1.4 + Heme oxygenase-1 (IFP1.4)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shu et al Science. 2009 May 8;324(5928):804-7. doi

Insert

Infrared Fluorescent Protein 1.4 + Heme oxygenase-1

Tags

Hexa-Histidine tag

Expression

Bacterial

Promoter

araBAD

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAW019-2

Plasmid

#85614

Purpose

Inducible dCas9 integration vector for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

dcas9

Expression

Bacterial

Promoter

PxylA (B. megaterium)

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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Plasma membrane-targeted optoPLD

Plasmid

#140114

Purpose

Encodes CRY2-mCherry-PLD-P2A-CIBN-CAAX for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pPS22 (pET23a-Cas10/Csm/csm2H6N)

Plasmid

#70038

Purpose

Bacterial expression of the S. epidermidis Cas10·Csm complex

Depositor

Luciano Marraffini

Article

Hatoum-Aslan et al J Biol Chem. 2013 Sep 27;288(39):27888-9

Insert

Cas10/Csm complex

Tags

6xHIS on N terminus of Csm2

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA(MS2)-puro-barcode

Plasmid

#89493

Purpose

Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

Depositor

Gary Hon

Article

Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

Insert

sgRNA (with MS2 loop)

Use

Lentiviral

Promoter

U6

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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Nanog-2A-mCherry

Plasmid

#48680

Purpose

For knock-in donor vector by CRISPR/CAS system

Depositor

Rudolf Jaenisch

Article

Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

Insert

Nanog (Nanog Mouse)

Use

CRISPR

Tags

Cherry

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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shRNA-DNMT1i(3'UTR)

Plasmid

#24951

Depositor

Paul Khavari

Article

Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

Insert

shRNA DNMT1i targeting the 3′UTR

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
TRE-UBC-hCD47-T2A-GFP

Plasmid

#205458

Purpose

lentiviral plasmid for expression of human CD47

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

hCD47 (CD47 Human)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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