We narrowed to 12,355 results for: Hal

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pAAV-RHO-IntC-dCas9C-VPR-polyA

Plasmid

#166692

Purpose

Express C-terminal part of split dCas9-VPR

Depositor

Stylianos Michalakis

Article

Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

Insert

IntC-dCas9C-VPR

Use

AAV and CRISPR

Expression

Mammalian

Promoter

short human rhodopsin promoter

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pHochTypeIII

Plasmid

#214039

Purpose

Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

Cas10

Use

CRISPR

Mutation

WT

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-ARNTL-shRNA1

Plasmid

#166913

Purpose

Lentivirus for inducible knockdown of human ARNTL(BMAL1)

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

ARNTL shRNA (BMAL1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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G-GECO1.2-Orai1

Plasmid

#73562

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

G-GECO1.2-Orai1 (ORAI1 Synthetic, Human)

Tags

G-GECO1.2

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAG/HA-DEST DICER

Plasmid

#19881

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Dicer 1, ribonuclease type III (DICER1 Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-YTHDF2

Plasmid

#38089

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

YTHDF2 YTH domain family, member 2 (YTHDF2 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pDONR223_HyPer7RgDAAO

Plasmid

#217746

Purpose

Donor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.

Depositor

Seth Parker

Article

Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

Insert

HyPer7 D amino acid oxidase

Tags

Nuclear export signal

Expression

Mammalian

Mutation

Fused DAAO to the C-terminus of HyPer7 using a Gl…

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pbZIP9:GFP-GUS

Plasmid

#172483

Purpose

GFP-GUS driven by the bZIP9 promoter

Depositor

Wolf Frommer

Article

Kim et al Plant Cell. 2021 May 5;33(3):511-530. do

Insert

bZIP9 promoter (BZIP9 Mustard Weed)

Tags

GFP and GUS

Expression

Plant

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-ARNTL-shRNA2

Plasmid

#166914

Purpose

Lentivirus for inducible knockdown of human ARNTL(BMAL1)

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

ARNTL shRNA (BMAL1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAG/HA-DEST TNRC6A

Plasmid

#19883

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Trinucleotide repeat containing 6A (TNRC6A Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-HNRNPR

Plasmid

#38067

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

HNRNPR heterogeneous nuclear ribonucleoprotein R (HNRNPR Human)

Tags

FLAG, HA, and His

Expression

Mammalian

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pLVNP3.0-PEmaxRH

Plasmid

#206884

Purpose

Expresses FLAG-tagged PEmax (lacking the RNAseH domain) fused to Gag through a linker sequence

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Insert

PEMax Delta RNAseH

Tags

FLAG

Promoter

CMV

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLVNP3.0-ABE8e

Plasmid

#206882

Purpose

Expresses FLAG-tagged ABE8e fused to Gag through a linker sequence

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Insert

ABE8e

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-KIAA1967

Plasmid

#38082

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

KIAA1967 (CCAR2 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mCerulean 156-239-GGGS

Plasmid

#162616

Purpose

Allows for transcription of control mCerulean fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mCerulean

Expression

Mammalian

Mutation

156-239

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST EIF2C2

Plasmid

#19888

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Eukaryotic translation initiation factor 2C, 2 (AGO2 Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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G-GECO1-Orai1

Plasmid

#73561

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

NM_032790.3 (ORAI1 Synthetic, Human)

Tags

G-GECO1

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-DDX54-FLAG/HA

Plasmid

#97062

Purpose

Expresses DDX54-FLAG/HA (C-terminal tagged) in mammalian cells

Depositor

Markus Landthaler

Article

Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

Insert

DDX54 (DDX54 Human)

Tags

FLAG/HA

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-CAD

Plasmid

#73566

Purpose

Soluble red fluorescent activator of Orai1 channels

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

mCherry-CAD (STIM1 342-448) (STIM1 Synthetic, Human)

Tags

6x His and mCherry

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-TRE-FH-TET3 short

Plasmid

#166916

Purpose

Lentivirus for inducible overexpression of human TET3 short isoform lacking CXXC domain (NM_001366022.1)

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

TET3 short isoform

Use

Lentiviral

Tags

HA Tag

Expression

Mammalian

Mutation

Changed nucleotide 18 (G) to (A) to delete FseI r…

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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