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pJL1 eforRed

Plasmid

#106320

Purpose

Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

eforRed

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL Hygro-NMYC TRF2 deltaB deltaM

Plasmid

#16355

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
Rc/CMV cyclin D1 HA

Plasmid

#8948

Depositor

Philip Hinds

Article

Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

Insert

cyclin D1 (CCND1 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pIntegrase_3

Plasmid

#60575

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_3

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LplA(W37V) Coumarin PRIME ligase

Plasmid

#25845

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LplA-W37V

Tags

Flag and His

Expression

Mammalian

Mutation

W37V

Available Since

July 27, 2010

Availability

Academic Institutions and Nonprofits only

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pJK_kanR_proJK1_PAD

Plasmid

#60630

Purpose

Constitutively expresses PAD (phenolic acid decarboxylase)

Depositor

Timothy Whitehead

Article

Tomek et al Biotechnol Bioeng. 2015 Mar;112(3):627-3

Insert

Phenolic acid decarboxylase padC (padC )

Expression

Bacterial

Mutation

K146Y

Promoter

proJK1

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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LV-TRE-p65 mouse MyoD-T2A-dsRedExpress2

Plasmid

#60627

Purpose

Expresses p65 mouse MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

p65 mouse MyoD

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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CFP_Rad_pcDNA4_HisMaxC

Plasmid

#41654

Depositor

Henry Colecraft

Article

Yang et al PLoS One. 2012;7(5):e37079. Epub 2012 Ma

Insert

Rad, GTP binding proteins (RGK) (Rrad Mouse)

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pEN_TRE_GFP

Plasmid

#25767

Purpose

Entry vector with TRE promoter driving GFP expression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

GFP

Available Since

July 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS-N2c(G)

Plasmid

#73481

Purpose

N2c Glycoprotein expression vector

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

N2c(G)

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only
pNL4-3 envFS eGFP gag2xFS

Plasmid

#101341

Purpose

1st frameshift is CG nucleotide insertion in MA at nt 832. 2nd is a CTAG addition in CA at nt 1508.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMDC32-Kar2-CFP-HDEL

Plasmid

#96989

Purpose

Express endoplasmic reticulum (ER) marker in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

Kar2-CFP-HDEL

Expression

Plant

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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SFTPB Promoter reporter

Plasmid

#88868

Purpose

Surfactant Protein B Gene Promoter Reporter

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

Surfactant protein B gene promoter (SFTPB Human)

Use

Luciferase

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2

Plasmid

#25752

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-Cerulean-YPT31

Plasmid

#18849

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

YPT31 (YPT31 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

Plasmid

#66708

Purpose

expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

Depositor

Charles Gersbach

Insert

S.Pyogenes VP64-dCas9-VP64

Use

Lentiviral and Synthetic Biology

Tags

VP64

Mutation

D10A and H840A

Promoter

hUbC

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pReporter_3

Plasmid

#60564

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_3

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pJDC181

Plasmid

#60853

Purpose

Expresses click beetle red luciferase

Depositor

Jeffrey Cirillo

Article

Chang et al PLoS One. 2014 Sep 29;9(9):e108341. doi:

Insert

Click beetle red luciferase (optimized for Mycobacteria)

Expression

Bacterial

Mutation

codon optimized for Mycobacteria

Promoter

hsp60

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCDF1-mAtf4-HT-puro

Plasmid

#101795

Purpose

Lentiviral vector containing C-terminally Halo-tag tagged mouse ATF4

Depositor

Seiichi Oyadomari

Insert

Activating transcription factor 4 (Atf4 Mouse)

Use

Lentiviral

Tags

Halo tag

Expression

Mammalian

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pReporter_4

Plasmid

#60565

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_4

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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