We narrowed to 89,999 results for: Mal;

1. EF1a_MYOD1_P2A_Hygro_Barcode

   Plasmid

   #120464

   Purpose

   Barcoded lentiviral vector to express MYOD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYOD1 (MYOD1 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-CAG-EGFP-P2A-FusionRed

   Plasmid

   #191108

   Purpose

   Mammalian cell line expression plasmids for production of EGFP and FusionRed proteins

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   pAAV-CAG-EGFP-P2A-FusionRed
   

   Use

   AAV

   Tags

   FusionRed

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   chicken β-actin promoter

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCherry-NES-SspB-MCS-P2A-iLIDcaax

   Plasmid

   #173869

   Purpose

   Mammalian expression of mCherry-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

   Depositor

   Takao Nakata

   Article

   Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

   Insert

   mCherry-NES-SspB-MCS-P2A-iLIDcaax
   

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-TWIN-STREP-FLAG-NPRL3

   Plasmid

   #164984

   Purpose

   NPRL3 expressing vector for mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

   Insert

   NPRL3 (NPRL3 Human)
   

   Tags

   2xSTREP FLAG

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCK-Drosha(UniProt)-FLAG-mEGFP

   Plasmid

   #164691

   Purpose

   Express Drosha-FLAG-mEGFP in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Insert

   Drosha (DROSHA Human)
   

   Tags

   FLAG and mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTS100 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-TEV-MCS

   Plasmid

   #199353

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-TEV-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagBFP-P2A-ALFA-TEV

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p2T-cmv-SpCas9-TadDE-BlastR

   Plasmid

   #193850

   Purpose

   Express TadDE in mammalian cells with BlastR selection, Tol2 integration

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadDE
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP-WT (AF41)

   Plasmid

   #21367

   Depositor

   Gregory Germino

   Article

   Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

   Insert

   autosomal dominant polycystic kidney disease type I (PKD1 Human)
   

   Tags

   EGFP and FLAG

   Expression

   Mammalian

   Mutation

   wild-type sequence*

   Available Since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Circular 100,50 (RAB7A)

   Plasmid

   #170117

   Purpose

   AAV vector carrying a guide RNA targeting the human RAB7A mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 100,50 guide RNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA

    Plasmid

    #47457

    Purpose

    LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    NLS(alpha-imp)-CRY2PHR-NLS-VP64
    

    Tags

    2A_GFP

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EF1a_SRY_P2A_Hygro_Barcode

    Plasmid

    #120485

    Purpose

    Barcoded lentiviral vector to express SRY in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    SRY (SRY Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 SP-His-mCherry-HRP-VhHGFP

    Plasmid

    #112157

    Purpose

    Expression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.

    Depositor

    Ben Giepmans

    Article

    de Beer et al Histochem Cell Biol. 2018 Mar;149(3):261

    Insert

    anti-GFP FLIPPER-body
    

    Tags

    His and Thrombin cleavage site

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcdna3 Flag Jip1b

    Plasmid

    #52123

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1(-)-ABE7.10

    Plasmid

    #120399

    Purpose

    Expresses ABE7.10 in mammalian cells

    Depositor

    Junjiu Huang

    Article

    Liang et al Nat Commun. 2019 Jan 8;10(1):67. doi: 10

    Insert

    ABE7.10
    

    Expression

    Mammalian

    Mutation

    Mammalian codon-optimized

    Promoter

    CMV

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Circular 200,100 with interspersed loops (SMAD4)

    Plasmid

    #180191

    Purpose

    AAV vector carrying a guide RNA targeting the human SMAD4 mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with interspersed loops
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLEX-FHH-MTORKinaseDomain

    Plasmid

    #120708

    Purpose

    Expresses Flag-HA-6xHIS-Mtor Kinase Domain fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    MTOR Kinase Domain (MTOR Human)
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Amino acids 1967-2549

    Promoter

    CMV

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSH231-EFS-Cas9-BlastR

    Plasmid

    #115146

    Purpose

    Safe harbor site 231 knock-in vector with Cas9-BlastR expression cassette

    Depositor

    Raymond Monnat, Michael Phelps

    Article

    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

    Insert

    Cas9-T2A-BlastR
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTS114 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199367

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-SUMOEu-ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RHBDD1 pmRFP-N2 (1390)

    Plasmid

    #62049

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    RHBDD1 (RHBDD1 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA5FRT/TO-UBE2QL1-Strep/HA

    Plasmid

    #124666

    Purpose

    Expresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2QL1 (UBE2QL1 Human)
    

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge