We narrowed to 6,241 results for: nls

1. pSL1315

   Plasmid

   #129527

   Purpose

   pDEF-SpCas9, Best (-458) Vector for CRISPRi of PyALBA4, PbEF1a promoter, 1st Generation

   Depositor

   Scott Lindner

   Article

   Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

   Insert

   dSpCas9
   

   Use

   Plasmodium, rodent-infectious species

   Tags

   NLS, 1xHA

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. p35SZ

   Plasmid

   #51513

   Purpose

   pMDC32 with Zif268:FokI followed by approximately 3kb of filler sequence

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Inserts

   Zif268:FokI
   3kb filler sequence
   

   Use

   Plant expression t-dna

   Tags

   NLS

   Promoter

   2x35S

   Available Since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLSLZ.D

   Plasmid

   #51496

   Purpose

   pLSL with Zif268:FokI coding sequence followed by us:NPTII repair template , Rep is not included on this plasmid

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Inserts

   Zif268:FokI
   us:NPTII repair template
   

   Use

   Plant t-dna plasmid

   Tags

   SV40 NLS

   Promoter

   virion-sense LIR and 2x35S

   Available Since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pdCas9-GFP-p65

   Plasmid

   #232630

   Purpose

   p65 activation domain coupled to catalytically inactive dCas9 for localization; contains GFP for labeling and as a spacer

   Depositor

   Karsten Rippe

   Insert

   dCas9-GFP-p65 (RELA Synthetic, Human)
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

   Plasmid

   #200389

   Purpose

   3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

   Depositor

   Debojyoti Chakraborty

   Insert

   PX458_3XHA_dFnCas9_KRAB_eGFP
   

   Use

   CRISPR

   Tags

   3xHA-NLS and SV40NLS-KRAB-T2A-EGFP

   Expression

   Mammalian

   Promoter

   CBH

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS42K_MCP-VP64_D-MS2

   Plasmid

   #177161

   Purpose

   Expresses transactivator (MCP-VP64) and scRNA (D-MS2) in yeast cells

   Depositor

   Gautam Dantas

   Article

   Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

   Inserts

   MCP-VP64
   D-MS2
   

   Tags

   SV40 NLS

   Expression

   Yeast

   Promoter

   ADH1 promoter and SNR52 promoter

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS42K_PCP-VP64_A-PP7

   Plasmid

   #177156

   Purpose

   Expresses transactivator (PCP-VP64) and scRNA (A-PP7) in yeast cells

   Depositor

   Gautam Dantas

   Article

   Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

   Inserts

   PCP-VP64
   A-PP7
   

   Tags

   SV40 NLS

   Expression

   Yeast

   Promoter

   ADH1 promoter and SNR52 promoter

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS42K_MCP-VP64_C-MS2

   Plasmid

   #177160

   Purpose

   Expresses transactivator (MCP-VP64) and scRNA (C-MS2) in yeast cells

   Depositor

   Gautam Dantas

   Article

   Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

   Inserts

   MCP-VP64
   C-MS2
   

   Tags

   SV40 NLS

   Expression

   Yeast

   Promoter

   ADH1 promoter and SNR52 promoter

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. EC0_4_VPR

   Plasmid

   #163716

   Purpose

   Plasmid encoding VPR (VP64 - SV40 NLS - p65 - Rta) as a Type 4a part to be used in the Dueber YTK system (MoClo kit)

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Insert

   VPR
   

   Use

   Synthetic Biology; Moclo vector level 0 (type 4a)

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. TC512

    Plasmid

    #164888

    Purpose

    Pmig-13::Zip(-)::QFAD::NLS::SL2::mCherry

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    zip-QFAD
    

    Expression

    Worm

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSL1211

    Plasmid

    #129521

    Purpose

    pDEF-SpCas9::GFP, For deletion of pyalba4, PyGAPDH promoter, 2nd Generation

    Depositor

    Scott Lindner

    Article

    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

    Insert

    SpCas9
    

    Use

    Plasmodium, rodent-infectious species

    Tags

    3XFlag-NLS

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEPOR0SP0008

    Plasmid

    #117535

    Purpose

    Level0 Golden Gate part: CDS, AsCas12a

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    AsCas12a (with stop codon)
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDS2

    Plasmid

    #70135

    Purpose

    CEN/ARS yeast shuttle vector with DS2 selection marker

    Depositor

    Morten Sommer

    Article

    Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

    Insert

    DS2
    

    Use

    Synthetic Biology

    Promoter

    AgTEF1

    Available Since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4.23-CD69 CaRE3 (minus strand)

    Plasmid

    #91845

    Purpose

    Luciferase reporter for CD69 enhancer (IGI-P0622)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    CD69 CaRE3 (minus strand) (CD69 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Missing 1 base from 13 base polyT tract beginning…

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL4.23-IL2RA CaRE4 (plus strand)

    Plasmid

    #91850

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0345)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL4.23-IL2RA CaRE4 scramble (plus strand)

    Plasmid

    #91852

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0347)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 scramble (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Sequence scrambled from ch10:6094588-6094934

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL4.23-IL2RA CaRE4 (minus strand)

    Plasmid

    #91849

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0626)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL4.23-IL2RA CaRE1 (plus strand)

    Plasmid

    #91837

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0614)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE1 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL4.23-CD69 CaRE3 (plus strand)

    Plasmid

    #91844

    Purpose

    Luciferase reporter for CD69 enhancer (IGI-P0621)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    CD69 CaRE3 (plus strand) (CD69 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Missing 1 base from 13 base polyT tract beginning…

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL4.23-IL2RA CaRE5 (plus strand)

    Plasmid

    #91841

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0618)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE5 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge