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pGL3-MTS-CCDB-N136-RsDddA-A-UGI-PURO

Plasmid

#205463

Purpose

A backbone containing a RsDddA fragment A for constructing mitochondria-targeted RsDdCBE expression plasmid

Depositor

Bin Shen

Article

Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

Insert

MTS; N136; ccdb; RsDddA-A; UGI

Expression

Mammalian

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-CCDB-N136-RsDddA-C-UGI-PURO

Plasmid

#205465

Purpose

A backbone containing a RsDddA fragment C for constructing mitochondria-targeted RsDdCBE expression plasmid

Depositor

Bin Shen

Article

Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

Insert

MTS; N136; ccdb; RsDddA-C; UGI

Expression

Mammalian

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pBDKRB2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

Plasmid

#101108

Purpose

Encodes dCas9(N) fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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Direct-seq_lentiGuide-Puro-tRNA-Tetra Loop-8A8G

Plasmid

#157985

Purpose

Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

Depositor

Lijia Ma

Article

Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

Insert

S. pyogenes sgRNA cassette (tRNA-Tetra-8A8G)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Flag-PolB(K244A/T304I)-Puro

Plasmid

#177144

Purpose

Lentiviral vector expressing Flag-PolB(K244A/T304I) and a puromycin resistance cassette

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-puro-BGHpA-TRE-pEF-tagRFP-T-NLS

Plasmid

#163083

Purpose

Donor vector for integration of TagRFP-T reporter at AAVS1 safe harbor site via TALENs

Depositor

Lacra Bintu

Article

Van et al Nat Commun. 2021 Jan 22;12(1):537. doi:

Insert

TagRFP-T

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dHgm4Cas13b-EGFP-NLS

Plasmid

#191360

Purpose

Overexpression of NLS-dHgm4Cas13b-EGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-dHgm4Cas13b-EGFP-NLS

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer

Plasmid

#86195

Purpose

U6 based expression of N meningitidis sgRNA

Depositor

Scot Wolfe

Article

Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

Insert

nmCas9 sgRNA cloning cassete

Use

Lentiviral

Promoter

U6

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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dnCEBP: pLenti-TRE-A-C/EBP-PGK-rtTAA-2A-Puro

Plasmid

#177201

Purpose

encoding a dominant negative form of CEBP under inducible promoter

Depositor

Markus Covert

Article

Kudo et al bioRxiv (2022)

Insert

dnCEBP (A-C/EBP)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCXCR4_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

Plasmid

#101114

Purpose

Encodes dCas9(N) fused to CXCR4

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

CXCR4-dCas9(C)-VP64

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAVPR2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

Plasmid

#101112

Purpose

Encodes dCas9(N) fused to AVPR2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

AVPR2-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pVEGFR2_TEV(N)_NES_TCS(Q'L)_FKBP_HA-dCas9(N)_P2A_Puro-WPRE

Plasmid

#101106

Purpose

Encodes FKBP-dCas9(N) fused to VEGFR2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR2-FKBP-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLPAR1_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

Plasmid

#101116

Purpose

Encodes dCas9(N) fused to LPAR1

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

LPAR1-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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hPlekhm1-Mid/pIRES puro Glue (Q513-E784)

Plasmid

#73455

Purpose

Express human middle part (Q513-E784) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pTRE3G-puro_V5-LOV-Tb-myc-MIRO1-TM-KQR (OMM)

Plasmid

#253797

Purpose

Express V5-LOV-Turbo2-myc-Miro1-TM-KQR (OMM) under TRE3G (tetracycline inducible) promoter

Depositor

Xinnan Wang

Article

Kwak et al bioRxiv [Preprint]. 2025 Dec 23:2025.12.

Insert

V5-LOV-Tb-myc-Miro1-TM-KQR (OMM) (RHOT1 Human)

Use

Lentiviral; Tetracycline inducible promoter

Expression

Mammalian

Promoter

pTRE_tetracycline inducible

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pBABE-IL18R1-QY-tTA-SV40-puro-T2A-TagBFP

Plasmid

#254088

Purpose

Retroviral transfer vector for IL18R1-tTA fusion gene expression, QY linker, puromycin selection marker, for CyCLoPs platform.

Depositor

Jun Huh

Article

Lu et al Cell. 2026 Feb 5;189(3):939-955.e26. doi

Insert

IL18R1-tTA

Use

Retroviral

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pBABE-IL17RE-QY-tTA-SV40-puro-T2A-TagBFP

Plasmid

#254082

Purpose

Retroviral transfer vector for IL17RE-tTA fusion gene expression, QY linker, puromycin selection marker, for CyCLoPs platform.

Depositor

Jun Huh

Article

Lu et al Cell. 2026 Feb 5;189(3):939-955.e26. doi

Insert

IL17RE-tTA

Use

Retroviral

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pBABE-IL1RAP-QY-tTA-SV40-puro-T2A-TagBFP

Plasmid

#254083

Purpose

Retroviral transfer vector for IL1RAP-tTA fusion gene expression, QY linker, puromycin selection marker, for CyCLoPs platform.

Depositor

Jun Huh

Article

Lu et al Cell. 2026 Feb 5;189(3):939-955.e26. doi

Insert

IL1RAP-tTA

Use

Retroviral

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pBABE-IL4RA-QY-tTA-SV40-puro-T2A-TagBFP

Plasmid

#254084

Purpose

Retroviral transfer vector for IL4RA-tTA fusion gene expression, QY linker, puromycin selection marker, for CyCLoPs platform.

Depositor

Jun Huh

Article

Lu et al Cell. 2026 Feb 5;189(3):939-955.e26. doi

Insert

IL4RA-tTA

Use

Retroviral

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pBABE-IL5RA-QY-tTA-SV40-puro-T2A-TagBFP

Plasmid

#254085

Purpose

Retroviral transfer vector for IL5RA-tTA fusion gene expression, QY linker, puromycin selection marker, for CyCLoPs platform.

Depositor

Jun Huh

Article

Lu et al Cell. 2026 Feb 5;189(3):939-955.e26. doi

Insert

IL5RA-tTA

Use

Retroviral

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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