We narrowed to 32,224 results for: uros

1. pGL3-MTS-G3635A-L17-Q2L7-T2176C-UGI-Puro

   Plasmid

   #209646

   Purpose

   To install m.G3635 mutation on mtDNA

   Depositor

   Bin Shen

   Article

   Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

   Insert

   L17-Q2L7-T2176C
   

   Expression

   Mammalian

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-MTS-G3635A-L17-FZY2-S100N-UGI-Puro

   Plasmid

   #209642

   Purpose

   To install m.G3635 mutation on mtDNA

   Depositor

   Bin Shen

   Article

   Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

   Insert

   L17-FZY2-S100N
   

   Expression

   Mammalian

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-MTS-G3635A-L17-FZY2-S100C-UGI-Puro

   Plasmid

   #209641

   Purpose

   To install m.G3635 mutation on mtDNA

   Depositor

   Bin Shen

   Article

   Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

   Insert

   L17-FZY2-S100C
   

   Expression

   Mammalian

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSB-CMV-MCS-puro PM-mKate2-P2A-GEM-LTB4mut

   Plasmid

   #202644

   Purpose

   Expresses the LTB4-insensitive control sensor GEM-LTB4mut along with the plasma membrane targeted mKate2 tag in mammalian cells

   Depositor

   Balázs Enyedi

   Article

   Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

   Insert

   PM-mKate2-P2A-GEM-LTB4mut
   

   Tags

   PM-mKate2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1072 pBabe puroL myr Akt K179M T308A S473A

   Plasmid

   #9015

   Depositor

   William Sellers

   Article

   DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

   Insert

   Akt1 K179M T308A S473A (AKT1 Human)
   

   Use

   Retroviral

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   K179M T308A S473A

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

   Plasmid

   #69546

   Purpose

   Tet-On system expressing MyoD-2A-GFP-2xNLS.

   Depositor

   Charles Gersbach

   Inserts

   eGFP
   Myod1 (Myod1 Mouse)
   

   Use

   Lentiviral

   Tags

   2x nuclear localization sequence and T2A "sk…

   Mutation

   Removed stop codon to fuse it to the 2A-eGFPnls i…

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-MTS-G3635A-R16-Q2L7-T2176N-UGI-Puro

   Plasmid

   #209649

   Purpose

   To install m.G3635 mutation on mtDNA

   Depositor

   Bin Shen

   Article

   Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

   Insert

   R16-Q2L7-T2176N
   

   Expression

   Mammalian

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-IRES-puro-NLS-NLS-dPba3Cas13b-3xGFP-NLS

   Plasmid

   #191369

   Purpose

   Overexpression of NLS-NLS-dPba3Cas13b-3xGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-NLS-dPba3Cas13b-3xGFP-NLS
   

   Tags

   3xGFP

   Expression

   Mammalian

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor

   Plasmid

   #184952

   Purpose

   Donor for both PiggyBac and Cp36 Recombinase

   Depositor

   Ami Bhatt, Lacra Bintu, Patrick Hsu, Michael Bassik

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   PuroR, P2A, mTagBFP2
   

   Expression

   Mammalian

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Histone H2B targeted Aurora B FRET sensor Kif2 substrate

    Plasmid

    #45216

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    Histone H2B-targeted Aurora B FRET sensor
    

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    May 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

    Plasmid

    #52419

    Purpose

    Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    ERAS (ERAS Human)
    

    Promoter

    CAGGS

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-IRES-puro-NLS-dAdmCas13d-EGFP-NLS-3xFlag

    Plasmid

    #132412

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dAdmCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    R269A; H274A; R837A; H842A

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-IRES-puro-NLS-NLS-dHgm4Cas13b-3xGFP-NLS

    Plasmid

    #191368

    Purpose

    Overexpression of NLS-NLS-dHgm4Cas13b-3xGFP-NLS in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-NLS-dHgm4Cas13b-3xGFP-NLS
    

    Tags

    3xGFP

    Expression

    Mammalian

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE

    Plasmid

    #168106

    Purpose

    PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning

    Depositor

    Erich Jarvis

    Article

    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

    Insert

    mCherry-KASH
    

    Use

    CRISPR and Cre/Lox

    Promoter

    CAG

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA

    Plasmid

    #47458

    Purpose

    LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    TALE (Neurog2)-GS-CIB1(NLS*,∆318-334)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE

    Plasmid

    #138144

    Purpose

    Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PguCas13b
    

    Use

    Lentiviral

    Tags

    NES

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216249

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminus

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgRANGAP.C1 (RANGAP1 Human)
    

    Use

    CRISPR; Endogenous tagging

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. AttB_[Kozak-mut]ACE2_IRES-mCherry-H2A-P2A-PuroR

    Plasmid

    #171595

    Purpose

    Weak human ACE2 abundance from Bxb1 landing pad

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Inserts

    ACE2 (ACE2 Human)
    mCherry
    H2A
    Pac
    

    Use

    Synthetic Biology; Promoterless bxb1 dna recombin…

    Expression

    Mammalian

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. N163 nLV EF-1a-Hp1a(CS)-Frbx2(Frb+FrbWobb)-V5-PGK-Puro

    Plasmid

    #44195

    Depositor

    Jerry Crabtree

    Article

    Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

    Insert

    Cbx5 (Cbx5 Mouse)
    

    Use

    Lentiviral

    Tags

    Frb, Hu Frb x2(WT Frb+codon Wobble Frb), and V5/e…

    Mutation

    N-term chromo domain removed

    Promoter

    EF-1a

    Available Since

    June 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAVS1-Puro CAG-CLTX CD32a ITAM CD3z CAR

    Plasmid

    #171965

    Purpose

    Donor plasmid for CLTX CD32a-ITAM CD3z CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

    Insert

    Synthetic CLTX CD32a ITAM CD3z CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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