We narrowed to 14,641 results for: NSI

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pcDNA GAL4 CREB 1-341

Plasmid

#22972

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

Insert

CREB

Tags

GAL4 DNA-binding domain

Expression

Mammalian

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pTCW002

Plasmid

#83522

Purpose

pheromone mediated destablised-GFP expression in yeast

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1-yEGFPCLN2PEST

Expression

Yeast

Promoter

pFUS1

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGL-Rbl2

Plasmid

#20885

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

Rbl2 (p130) 3'UTR (Rbl2 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pJK220

Plasmid

#71123

Purpose

Expression of E. coli pantotheinate kinase (H6PanK, R175H mutant) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

pantothenate kinase

Tags

H6

Expression

Bacterial

Mutation

Arg175 is mutated to a His

Promoter

T7

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBT225_(pCA-tdT3Myc)

Plasmid

#36873

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tdTomato-3Myc

Tags

3 Myc tags

Expression

Mammalian

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pGL-Rbl1UTR

Plasmid

#20884

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

Rbl1 (p107) 3'UTR (Rbl1 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pJLI-sup35

Plasmid

#1210

Depositor

Susan Lindquist

Article

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 5' and 3' flanking regions (SUP35 Budding Yeast)

Expression

Yeast

Mutation

sup35 gene is not included in this plasmid. Only…

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 14-3-3 epsilon K49E

Plasmid

#11945

Depositor

Michael Yaffe

Article

Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

Insert

Ywhae (Ywhae Rat)

Tags

GST

Expression

Bacterial

Mutation

K49E

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only
pFLLFLN

Plasmid

#40030

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

FRT-Lox5171-Lox2272-FRT-loxP
Neo

Expression

Bacterial and Mammalian

Promoter

None and SV40

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-T-protein

Plasmid

#12652

Depositor

SeWon Suh

Article

Lee et al J Biol Chem. 2004 Nov 26. 279(48):50514-

Insert

T-protein

Expression

Bacterial

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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YEplac181-GAL11

Plasmid

#8628

Depositor

Jeff Strathern

Article

Malagon et al Genetics 2004 Mar;166(3):1215-27.

Insert

GAL11 (GAL11 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only

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PILAR

Plasmid

#22851

Depositor

Jose Conejo-Garcia

Article

Huarte et al Blood. 2008 Aug 15. 112(4):1259-68.

Insert

PILAR (CLEC2A Human)

Use

Cloning vector

Available Since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only
pBSc + Shal

Plasmid

#16142

Depositor

Larry Salkoff

Article

Fawcett et al J Biol Chem. 2006 Oct 13. 281(41):30725-

Insert

Voltage-gated K+ channels (Y73B6BL.19 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
p259 pCMV-CRE-M (BglII)

Plasmid

#12493

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

Insert

CRE-M

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
Sus1/PET32a

Plasmid

#26963

Depositor

Cynthia Wolberger

Article

Samara et al Science. 2010 May 21. 328(5981):1025-9.

Insert

Sus1 (SUS1 Budding Yeast)

Tags

His6 and Trx

Expression

Bacterial

Mutation

with TEV site to cleave the N-terminal his+Trx tag

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pUC19_TTE1564

Plasmid

#61000

Purpose

T7-transcription template for Tte mRNA used for in vitro translation

Depositor

Nils Walter

Article

Rinaldi et al Nat Commun. 2016 Jan 19;7:8976. doi: 10.

Insert

TTE1564 transcript

Use

Unspecified

Promoter

T7

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pPICZC_CeTRIC-B2_Del

Plasmid

#87345

Purpose

Inducible expression of CeTRIC-B2 in P.Pichia strains

Depositor

Zhenfeng Liu

Article

Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

Insert

TRIC-B2

Tags

polyhistidine

Expression

Yeast

Mutation

Δ253-313

Promoter

AOX1

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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MetNI (E166Q in MetN)

Plasmid

#78320

Purpose

Methionine ABC Transporter

Depositor

Douglas Rees

Article

Johnson et al Protein Sci. 2012 Jan;21(1):84-96. doi:

Inserts

MetI
MetN

Tags

10x Histidine Tag and 6x Histidine Tag

Expression

Bacterial

Mutation

E166Q

Promoter

T7 Promoter

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pUC19_extendedTTE1564

Plasmid

#60999

Purpose

T7-transcription template for Tte mRNA used in SiM-KARTS

Depositor

Nils Walter

Article

Rinaldi et al Nat Commun. 2016 Jan 19;7:8976. doi: 10.

Insert

TTE1564 transcript

Use

Unspecified

Promoter

T7

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pMZ036

Plasmid

#66973

Purpose

Expresses zebrafish Chk1

Depositor

Michael Lampson

Article

Zhang et al Cell Cycle. 2014;13(24):3828-38. doi: 10

Insert

zebrafish Chk1

Use

Zebrafish mrna injection

Expression

Mammalian

Mutation

Q341H mutation compared to GenBank reference sequ…

Promoter

CMV

Available Since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pJK227

Plasmid

#71126

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Tags

GyrA intein

Expression

Bacterial

Promoter

T7

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pJK136

Plasmid

#71122

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AarA) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pMZ039

Plasmid

#67757

Purpose

Expresses N-terminal kinase domain of Chk1 with C-term GFP fluorescent tag

Depositor

Michael Lampson

Article

Zhang et al Cell Cycle. 2014;13(24):3828-38. doi: 10

Insert

zebrafish Chk1

Tags

GFP

Mutation

only encodes N-term kinase domain (amino acids 1-…

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pFLN

Plasmid

#40029

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

FRT-loxP
Neo

Expression

Bacterial and Mammalian

Promoter

None and SV40

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pOX + Shaker1c

Plasmid

#16140

Depositor

Larry Salkoff

Article

Fawcett et al J Biol Chem. 2006 Oct 13. 281(41):30725-

Insert

Voltage-gated K+ channels (ZK1321.2 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pOX + Shaker1d

Plasmid

#16141

Depositor

Larry Salkoff

Article

Fawcett et al J Biol Chem. 2006 Oct 13. 281(41):30725-

Insert

Voltage-gated K+ channels (ZK1321.2 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pDGB1_alpha1

Plasmid

#68224

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMito-PAGFP-FRB.

Plasmid

#60005

Purpose

Expresses MitoTrap tagged with photo-activatable GFP in mammalian cells.

Depositor

Stephen Royle

Article

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Type

Empty backbone

Use

Knocksideways

Tags

FRB and PAGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_PGK_antiCD19_synNotch_Gal4VP64

Plasmid

#79125

Purpose

Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch Gal4DBDVP64 Receptor (PGK promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

antiCD19_synNotch_Gal4VP64

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 FLII12Pglu-700uDelta6

Plasmid

#17866

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLII12Pglu-700uDelta6

Tags

Citrine

Expression

Mammalian

Mutation

700uDelta6, ECFP insert in mglB

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pHR_pGK_LaG17_synNotch_Gal4VP64

Plasmid

#79127

Purpose

Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch Gal4DBDVP64 Receptor (PGK promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

LaG17_synNotch_Gal4DBDVP64

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-FKBP:mCherry-Rab5a

Plasmid

#72901

Purpose

Early endosome-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Rab5a (RAB5A Human)

Use

Lentiviral

Tags

FKBP(F36V) and mCherry

Expression

Mammalian

Mutation

fragment a.a. 2-215

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pIntegrase_12

Plasmid

#60583

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_12

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSIL-eGFP

Plasmid

#52675

Purpose

shRNA silencing plasmid with eGFP transfection marker

Depositor

Johannes Hell

Article

Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

Type

Empty backbone

Use

RNAi

Tags

CMV-EGFP

Promoter

CMV and U6

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pmGFP-Sec16L

Plasmid

#15776

Depositor

Benjamin Glick

Article

Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

Insert

Sec16L (SEC16A Human)

Tags

mGFP

Expression

Mammalian

Mutation

N/A

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-FKBP:mCherry-BclXLc33

Plasmid

#72905

Purpose

Outer mitochondrial membrane-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Bl2-like protein 1, isoform Bcl-X(L) (fragment)

Use

Lentiviral

Tags

FKBP, mCherry

Expression

Mammalian

Mutation

33 C-terminal a.a.

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_PGK_antiCD19_synNotch_TetRVP64

Plasmid

#79126

Purpose

Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch TetRVP64 Receptor (PGK promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

antiCD19_synNotch_TetRVP64

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_SFFV_LaG17_synNotch_TetRVP64

Plasmid

#79128

Purpose

Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch TetRVP64 Receptor (SFFV promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

LaG17 GFP nanobody synNotch TetRVP64

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFLAG-TNKS-2

Plasmid

#34691

Depositor

Nai-Wen Chi

Article

Sbodio et al Biochem J. 2002 Feb 1;361(Pt 3):451-9.

Insert

TNKS2 (TNKS2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
Myc-TEAD4

Plasmid

#24638

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

TEAD-4 (TEAD4 Human)

Tags

Myc

Expression

Mammalian

Available Since

May 13, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge

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