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Plasmid#168984PurposeExpresses RFP cDNA and SAE1 shRNA 1 in mammalian cellsDepositorInsertSUMO1 Activating Enzyme Subunit 1 (SAE1 Human)
UseTagsExpressionMammalianMutationPromoterUbc promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Control-cx43.4-48H-pPRISM
Plasmid#117801PurposeControl to test technique on a target that is known to workDepositorInsertTagRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-TagRFP-cmlc2-mRFP
Plasmid#117769PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-gcry-eGFP
Plasmid#117785PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertmRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-TagRFP-cmlc2-eGFP
Plasmid#117770PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-gcry-mRFP
Plasmid#117784PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertmRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Cre-gcry-mRFP
Plasmid#117790PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertCre
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2-msfGFP
Plasmid#174492Purposebacterial expression of human SEPT2 fused to monomeric superfolder GFPDepositorInsertSEPTIN2 (SEPTIN2 Human)
UseTagsHis6-TEV and msfGFPExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pnCS_SEPT7_SEPT9_i3-TEV-Strep
Plasmid#174501Purposebacterial co-expression of human SEPT7 and of human SEPT9_i3DepositorUseTagsTEV-StrepExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5-LYCHOS (GPR155) WT-myc
Plasmid#199684PurposeTransient expression of LYCHOS (GPR155) WTDepositorInsertGPR155 (GPR155 Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-LYCHOS (GPR155) 4CA-HA
Plasmid#199677PurposeTransient expression of LYCHOS (GPR155) C595.604.629.638A mutantDepositorInsertGPR155 (GPR155 Human)
UseTagsHAExpressionMammalianMutationC595.604.629.638 mutated to APromoterCMVAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX LYCHOS-FLAG-TiD
Plasmid#199747PurposeLentiviral construct to stably express LYCHOS (GPR155) TurboIDDepositorInsertLYCHOS (GPR155 Human)
UseTagsFlag and TurboIDExpressionMammalianMutationPromoterEF1aAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCB268
Plasmid#53363PurposepcDNA3.0-based plasmid encoding fDHFR-UbK48R-Aβ42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.DepositorUseTags13xMyc, Flag, and HAExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Cre-cmlc2-mRFP
Plasmid#117793PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertCre
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5 LYCHOS (GPR155) ∆Permease-HA
Plasmid#199686PurposeTransient expression of LYCHOS (GPR155) ∆PermeaseDepositorInsertGPR155 (GPR155 Human)
UseTagsHAExpressionMammalianMutationDeletion from amino acid 1-369PromoterCMVAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5 LYCHOS ∆LED-HA
Plasmid#199682PurposeTransient expression of LYCHOS (GPR155) ∆LED-HADepositorInsertGPR155 (GPR155 Human)
UseTagsHAExpressionMammalianMutationDeletion from amino acid 556-651PromoterCMVAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-LYCHOS (GPR155) FP>IA-HA
Plasmid#199661PurposeTransient expression of LYCHOS (GPR155) FP>IA mutantDepositorInsertGPR155 (GPR155 Human)
UseTagsHAExpressionMammalianMutationF43 and P44 mutated to I and APromoterCMVAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-gcry-TagBFP
Plasmid#117783PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertmRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Cre-gcry-TagBFP
Plasmid#117789PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertCre
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Cre-cmlc2-TagBFP
Plasmid#117792PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertCre
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-cmlc2-eGFP
Plasmid#117788PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertmRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn INTRON HaloTag-STIM1 WPRE
Plasmid#236233PurposeAAV expression of mouse STIM1 internally tagged with HaloTagDepositorInsertHaloTag-Stim1 (Stim1 Mouse)
UseAAVTagsHaloTagExpressionMutationPromoterhuman Synapsin 1Available sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE
Plasmid#236231PurposeAAV expression of a fluorescent marker, mTagBFP2, fused to the plasma membrane targeting sequence CAAX2DepositorInsertmTagBFP2 fused to the plasma membrane targeting sequence CAAX2
UseAAVTagsmTagBFP2ExpressionMutationPromoterhuman Synapsin 1Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiSIV hSyn HA-NLS-SpCas9-NLS WPRE
Plasmid#236242PurposeLentiviral expression of SpCas9DepositorInsertSpCas9
UseLentiviralTagsExpressionMutationPromoterhuman Synapsin 1Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn INTRON mEmerald-STIM1 WPRE
Plasmid#236234PurposeAAV expression of mouse STIM1 internally tagged with mEmeraldDepositorInsertmEmerald-Stim1 (Stim1 Mouse)
UseAAVTagsmEmeraldExpressionMutationPromoterhuman Synapsin 1Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2
Plasmid#236246PurposeEndogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membraneDepositorInsertgRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2
UseAAVTagsmTagBFP2ExpressionMutationPromoterU6 and human Synapsin1Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
macroH2A2-V192F-GFP (pc5116)
Plasmid#223439PurposeMutation of valine 192 to phenylalanine (V192F) in macroH2A2.DepositorUseTagsGFPExpressionMammalianMutationMutation of valine 192 to phenylalanine (V192F)PromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Myc-BirA*-CAMK2D
Plasmid#221686PurposeMammalian expression of Myc-tagged BirA*-CAMK2D fusion protein for BioIDDepositorInsertCAMK2D (CAMK2D Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Myc-BirA*-RNF8-C403S
Plasmid#221685PurposeMammalian expression of Myc-tagged BirA*-RNF8 (C403S) fusion protein for BioIDDepositorInsertRNF8 (RNF8 Human)
UseTagsExpressionMammalianMutationC403SPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Myc-BirA*-RNF8-R42A
Plasmid#221684PurposeMammalian expression of Myc-tagged BirA*-RNF8 (R42A) fusion protein for BioIDDepositorInsertRNF8 (RNF8 Human)
UseTagsExpressionMammalianMutationR42APromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4.1-Myc-RNF8-4A
Plasmid#221682PurposeMammalian expression of Myc-tagged human RNF8 (R477A/E478A/R479A/K480A)DepositorInsertRNF8 (RNF8 Human)
UseTagsExpressionMammalianMutationR477A, E478A, R479A, K480APromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4.1-Myc-RNF8-3A
Plasmid#221681PurposeMammalian expression of Myc-tagged human RNF8 (R477A/E478A/R479A)DepositorInsertRNF8 (RNF8 Human)
UseTagsExpressionMammalianMutationR477A, E478A, R479APromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-hALDOA D34S
Plasmid#210813PurposeTo reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)DepositorInsertALDOA (ALDOA Human)
UseAdenoviralTagsHAExpressionMutationresidues 34 of ALDOA mutated to serinePromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hALDOA D34S
Plasmid#210807PurposeTo reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)DepositorInsertALDOA (ALDOA Human)
UseLentiviralTagsHAExpressionMutationresidues 34 of ALDOA mutated to serinePromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO shLuc/FLAG Metap2
Plasmid#209316PurposeLentiviral construct expressing FLAG Metap2 and shRNA against Luciferase (control shRNA)DepositorInsertMETAP2 (METAP2 Human, human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterPGKAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO shLYCHOS/LYCHOS WT-FLAG
Plasmid#209315PurposeLentiviral construct expressing shRNA against LYCHOS and LYCHOS WT-FLAG (resistant to shRNA)DepositorInsertLYCHOS (GPR155 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterPGKAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO shLYCHOS/FLAG Metap2
Plasmid#209314PurposeLentiviral construct expressing FLAG Metap2 and shRNA against LYCHOSDepositorInsertLYCHOS (GPR155 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterPGKAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB-CG-LYCHOS WT-HA-GFP
Plasmid#199687PurposeExpression of LYCHOS WT in insect cellsDepositorInsertLYCHOS (GPR155 Human)
UseTags10xHis, EGFP, HA, HRV 3C, and HisExpressionInsectMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-LYCHOS (GPR155) ∆DEP-HA
Plasmid#199683PurposeTransient expression of LYCHOS (GPR155) ∆DEP-HADepositorInsertGPR155 (GPR155 Human)
UseTagsHAExpressionMammalianMutationDEP domain deletedPromoterCMVAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5 LYCHOS (GPR155) Y551A-HA
Plasmid#199676PurposeTransient expression of LYCHOS (GPR155) Y551A mutantDepositorInsertGPR155 (GPR155 Human)
UseTagsHAExpressionMammalianMutationY551 mutated to APromoterCMVAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a LZ LED WT-FLAG
Plasmid#199679PurposeBacterial expression of Leucine Zipper (LZ) LYCHOS LED-FlagDepositorInsertLeucine zipper LYCHOS LED WT (GPR155 Human)
UseTags6xHis and FlagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-LYCHOS (GPR155) P44A-HA
Plasmid#199675PurposeTransient expression of LYCHOS (GPR155) P44A mutantDepositorInsertGPR155 (GPR155 Human)
UseTagsHAExpressionMammalianMutationP44 mutated to APromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-LYCHOS (GPR155) E48Q-HA
Plasmid#199662PurposeTransient expression of LYCHOS (GPR155) E48Q mutantDepositorInsertGPR155 (GPR155 Human)
UseTagsHAExpressionMammalianMutationE48 mutated to QPromoterCMVAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-LYCHOS (GPR155) F43I-HA
Plasmid#199674PurposeTransient expression of LYCHOS (GPR155) F43I mutantDepositorInsertGPR155 (GPR155 Human)
UseTagsHAExpressionMammalianMutationF43 mutated to IPromoterCMVAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-DSep1-msfGFP
Plasmid#174494Purposebacterial expression of Drosophila Sep1 fused to monomeric superfolder GFPDepositorInsertSep1 (Sep1 Fly)
UseTagsHis6-TEV and msfGFPExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-SAE1_sh3
Plasmid#168985PurposeExpresses RFP cDNA and SAE1 shRNA 3 in mammalian cellsDepositorInsertSUMO1 Activating Enzyme Subunit 1 (SAE1 Human)
UseTagsExpressionMammalianMutationPromoterUbc promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCVhyg-HA-FLAG_Ubc9_C93A
Plasmid#164937PurposeExpresses FLAG & HA tagged human Ubiquitin Conjugating Enzyme 9 (UBC9) _C93A mutant cDNADepositorInserthuman UBC9 (UBE2I Human)
UseRetroviralTagsFLAG & HA tagsExpressionMutationPromoterPGK promoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
JDW 249 (pTol2-Dll4-F2-ETS#2-WT-8X-E1b-EGFP)
Plasmid#156417Purpose8X copies of the ETS#2 site of the murine Dll4 intronic enhancer and a minimal E1b reporter driving expression of EGFP flanked by Tol2 sitesDepositorInsertmurine Dll4 F2-6/F8 ETS site B/ site #2, 8X
UseZebrafish transgenesisTagsExpressionMutationPromoterE1b min pro/b-globin intronAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-cmlc2-mRFP
Plasmid#117787PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertmRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
ptrc99a-GPPS-CSstr-ispA*[JBEI-15060]
Plasmid#100962PurposeContains genes encoding GPP synthase, cineole synthase and IspA(S81F)DepositorInsertGPPS-CSstr-ispA*
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only