1. p426 103Q GPD

   Plasmid

   #1184

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. pDSHA-ARF6-Myc

   Plasmid

   #67352

   Purpose

   Mammalian expression of hArf6 with C-term Myc tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF6 (ARF6 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pDEST40-ARF4-V5-His6

   Plasmid

   #67434

   Purpose

   Mammalian expression of hArf4 with C-term V5 and His6 tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF4 (ARF4 Human)
   

   Use

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pDEST40-ARF1-V5-His6

   Plasmid

   #67432

   Purpose

   Mammalian expression of hArf1 with C-term V5 and His6 tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF1 (ARF1 Human)
   

   Use

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pDEST40-ARF3-V5-His6

   Plasmid

   #67433

   Purpose

   Mammalian expression of hArf3 with C-term V5 and His6 tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF3 (ARF3 Human)
   

   Use

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. actc1b-mKate2-ARF1-DN

   Plasmid

   #109536

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to dominant negative ARF1

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   ARF1 (arf1 Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Expression

   Mutation

   DN

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. actc1b-mKate2-ARF6

   Plasmid

   #109537

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to ARF6

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   ARF6 (arf6a Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. actc1b-mKate2-ARF6-DN

   Plasmid

   #109538

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to dominant negative ARF6

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   ARF6 (arf6a Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Expression

   Mutation

   DN

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDEST47-ARF6

   Plasmid

   #67457

   Purpose

   Mammalian expression of hArf6 with stop codon (native expression)

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF6 (ARF6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pDEST47-ARF1

   Plasmid

   #67453

   Purpose

   Mammalian expression of hArf1 with stop codon (native expression)

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF1 (ARF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pDONR221-ARF1

    Plasmid

    #67306

    Purpose

    hArf1 with stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF1 (ARF1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

11. HA-MAP4K1

    Plasmid

    #229396

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MAP4K1 (MAP4K1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-RhoA*

    Plasmid

    #198716

    Purpose

    Expresses gRNA resistant RhoA under the CAG promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    RhoA (Rhoa Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    It has mutations that is resistant to the RhoA gR…

    Promoter

    CAG

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJET-CMV-GFP-P2A-K20-P2A-mCherry

    Plasmid

    #185618

    Purpose

    Ribosomal stalling reporter: positive control

    Depositor

    Sabine Fuchs

    Article

    Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

    Insert

    GFP-K20(AAA)-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJET-CMV-GFP-P2A-K0-P2A-mCherry

    Plasmid

    #185619

    Purpose

    Ribosomal stalling reporter: empty

    Depositor

    Sabine Fuchs

    Article

    Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

    Insert

    GFP-empty-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJET-CMV-GFP-P2A-I15-P2A-mCherry

    Plasmid

    #185620

    Purpose

    Ribosomal stalling reporter: 15x isoleucine

    Depositor

    Sabine Fuchs

    Article

    Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

    Insert

    GFP-I15-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMAL-MBP-FRB-RhoA/N12

    Plasmid

    #214298

    Purpose

    Express split-RhoA fragment fused with FRB and MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Split-RhoA fragment fused with FRB (RHOA Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21b-RhoA/13C-FKBP-MBP

    Plasmid

    #214299

    Purpose

    Express split-RhoA fragment fused with FKBP and MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Split-RhoA fragment fused with FKBP (RHOA Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    RhoA-Q63L

    Promoter

    T7

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GPR62-DuET

    Plasmid

    #213288

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR62 (GPR62 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TOMM20-mTurquoise-RhoA-G14V-deltaCaaX

    Plasmid

    #176123

    Purpose

    Constitutively active, mitochondrial localized RhoA

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    RHOA (RHOA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G14V

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p3E-ARF1-DN

    Plasmid

    #109590

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative ARF1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ARF1 (arf1 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    dominant negative

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge