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Showing: 361 - 380 of 2109 results

  1. pLV-EGFP-PolB-(K35A/K68A/K72A)-PAMmut-Hygro

    Plasmid
    #176089
    Purpose
    EGFP fused the N-terminus of POLB containing the mutations Lys35Ala, Lys68Ala, and Lys72Ala, a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutations in Lys35 to Ala, Lys68 to Ala, and Lys7…
    Promoter
    CMV
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176089-plasmid-map-sequence-id-352910

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  2. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(PAMmut)

    Plasmid
    #176139
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176139-plasmid-map-sequence-id-353012

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  3. pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-G60A)

    Plasmid
    #176144
    Purpose
    EGFP fused to the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Gly60Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB-T2A-SIRT6 (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Gly60 to Ala, and a mutation in the P…
    Promoter
    EF1A
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176144-plasmid-map-sequence-id-353152

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  4. pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-R65A)

    Plasmid
    #176143
    Purpose
    EGFP fused the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Arg65Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB-T2A-SIRT6 (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Arg65 to Ala, and a mutation in the P…
    Promoter
    EF1A
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176143-plasmid-map-sequence-id-353151

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  5. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(D256A/PAMmut)

    Plasmid
    #176141
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutation Asp256Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Myc-tagged PolB with mutation in Asp256 to Ala, a…
    Promoter
    EF1A
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176141-plasmid-map-sequence-id-353041

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  6. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(K35A/K68A/K72/PAMmut)

    Plasmid
    #176140
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutations Lys35Ala, Lys68Ala, Lys72Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resis
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Myc-tagged PolB with mutations Lys35 to Ala, Lys6…
    Promoter
    EF1A
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176140-plasmid-map-sequence-id-353035

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  7. CSH130+pRCPam

    Bacterial Strain
    #195859
    Purpose
    WT E. coli strain transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Serves as control for suppressor strains. No color when grown with or without rhamnose.
    Depositor
    Gregory Phillips
    Article
    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)
    Bacterial Resistance
    Chloramphenicol
    Species
    Acropora millepora
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  8. XA106+pRCPam

    Bacterial Strain
    #195858
    Purpose
    E. coli nonsense suppressor strain (leuX) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows magenta when CP is induced with rhamnose.
    Depositor
    Gregory Phillips
    Article
    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)
    Bacterial Resistance
    Chloramphenicol
    Species
    Acropora millepora
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  9. XA103+pRCPam

    Bacterial Strain
    #195857
    Purpose
    E. coli nonsense suppressor strain (tyrT) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. No color when grown with or without rhamnose.
    Depositor
    Gregory Phillips
    Article
    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)
    Bacterial Resistance
    Chloramphenicol
    Species
    Acropora millepora
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  10. XA101+pRCPam

    Bacterial Strain
    #195856
    Purpose
    E. coli nonsense suppressor strain (serU) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows salmon when CP is induced with rhamnose.
    Depositor
    Gregory Phillips
    Article
    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)
    Bacterial Resistance
    Chloramphenicol
    Species
    Acropora millepora
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  11. XA92+pRCPam

    Bacterial Strain
    #195855
    Purpose
    E. coli nonsense suppressor strain (glnX) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows purple when CP is induced with rhamnose.
    Depositor
    Gregory Phillips
    Article
    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)
    Bacterial Resistance
    Chloramphenicol
    Species
    Acropora millepora
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  12. pGG184

    Plasmid
    #165604
    Purpose
    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    'CAGCG' PAM replacing the 'CGGCG&#…
    Promoter
    lac
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165604-plasmid-map-sequence-id-334628

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  13. pGG198

    Plasmid
    #165605
    Purpose
    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFP
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    hEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Secondary protospacer ('CGGCG' PAM) ins…
    Promoter
    lac
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    165605-plasmid-map-sequence-id-338038

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  14. pxCas9CR4

    Plasmid
    #111656
    Purpose
    PCas9CR4 with the xCas9 mutations allowing NG PAM sites
    Depositor
    Christopher Reisch
    Insert
    TetR and xCas9
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Bacterial
    Mutation
    xCas9 mutations
    Promoter
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111656-plasmid-map-sequence-id-217189

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  15. pTPH405c

    Plasmid
    #185726
    Purpose
    Directed evolution of Cas9 adenine base editors with altered PAM compatibility
    Depositor
    David Liu
    Article
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Insert
    gIII(N)-NpuN-NpuC-gIII(C) single PAM linker
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    185726-plasmid-map-sequence-id-365709

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Showing: 361 - 380 of 2109 results