We narrowed to 1,056 results for: Psp
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Plasmid#148853PurposeMammalian Expression of HsTNRC6B-sgRNA3DepositorInsertHsTNRC6B-sgRNA3
UseCRISPRExpressionMammalianAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO1_AH
Plasmid#148854PurposeMammalian Expression of HsAGO1-sgRNADepositorAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-V2.0-sgHsNot1_AH
Plasmid#148856PurposeMammalian Expression of HsNot1-sgRNADepositorAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-V2.0-sgHsPatL_AH
Plasmid#148857PurposeMammalian Expression of HsPatL1-sgRNADepositorInsertHsPatL1-sgRNA
UseCRISPRExpressionMammalianAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-V2.0-sgHsTNRC6C_AH
Plasmid#148858PurposeMammalian Expression of HsTNRc6C-sgRNADepositorInsertHsTNRc6C-sgRNA
UseCRISPRExpressionMammalianAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::HA-RfA
Plasmid#186404PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-pFOGc::RfA
Plasmid#186397PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA
Plasmid#186396PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of a regulatory sequence cloned in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA-HA
Plasmid#186405PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
PDEST-pSP172BSSPE-pFOGc::RfA-HA
Plasmid#186406PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMS19: pHelper(PmcCAST)_PmcPSP1_ΔTniQ
Plasmid#168152PurposeInducible expression of PmcCAST proteins (except TniQ) with crRNA targeting PmcPSP1.DepositorInsertsPmcCAST minimal CRISPR array (with spacer for PmcPSP1)
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS2: pHelper(AvCAST)_AvPSP1_ΔTniQ
Plasmid#168134PurposeInducible expression of AvCAST proteins (except TniQ) with crRNA targeting AvPSP1.DepositorInsertsAvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB and TnsC)
AvTnsD
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSP64-EMTB-GFP-ePDZ
Plasmid#135600PurposeExpression of EMTB-GFP-ePDZ mRNA for microinjection for use of optogenetics in S. purpuratusDepositorInsertEMTB-GFP-ePDZ
UseIn vitro transcription (mrna synthesis)TagsEGFP and ePDZPromoterUnknownAvailable SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP64-Lifeact-GFP-ePDZ
Plasmid#135602PurposeExpression of Lifeact-GFP-ePDZ mRNA for microinjection to be used for optogenetics in S. purpuratusDepositorInsertLifeact-GFP-ePDZ
UseIn vitro transcription (mrna synthesis)TagsEGFP and ePDZPromoterUnknownAvailable SinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
psp-161-si2(sip1090)
Plasmid#58802Purposeis a lentiviral vector coding the shRNA si2 (sip1090) and the gene of resistance to puromycin.DepositorInsertsi2
UseLentiviralPromoterhuman H1Available SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSPL10F13R13::pBIB-BAS (gSPL10)
Plasmid#27407DepositorInsertSPL10 (AT1G27370 Mustard Weed)
UseUsed to create transgenic linesAvailable SinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
6mpSPL10F13R13::pBIB-BAS (6mSPL10)
Plasmid#27408DepositorInsertSPL10 mutant (AT1G27370 Mustard Weed)
UseUsed to create transgenic linesMutationmiR156 target sites of SPL10 mutatedAvailable SinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSP64T XAR1-myc (DM#121)
Plasmid#15049DepositorAvailable SinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Psptdh3-ACO-Tagtef1
Plasmid#127734PurposeYeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.DepositorInsertaconitase
UseSynthetic BiologyExpressionYeastPromoterPsptdh3AvailabilityAcademic Institutions and Nonprofits only -
pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover
Plasmid#196829PurposeExpress dpdpCas13b with AAAA mutation and CloverDepositorInsertdpspCas13b(AAAA)
Tags3XFLAG and NESExpressionMammalianMutationK367A, D369A and K370AAvailable SinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only