We narrowed to 3,940 results for: SPL

1. pAAV-CMV-BD10-SAS620-3’dCas9-VPR-synpA

   Plasmid

   #216323

   Purpose

   Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

   Depositor

   Elvir Becirovic

   Article

   Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

   Insert

   Split Cas9-VPR + splice acceptor site
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-3xsgMyo7b-CMV-5’dCas9-SDS-BD10-SV40pA

   Plasmid

   #216322

   Purpose

   Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

   Depositor

   Elvir Becirovic

   Article

   Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

   Insert

   Split Cas9-VPR + splice donor site + gRNAs targeting the murine Myo7b locus for transactivation
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET11a-N-DiB2

   Plasmid

   #168472

   Purpose

   N-fragment of the DiB2‐split protein. This plasmid needs to be co-transformed with Plasmid 168257: pMRBad-C-wtBlc to obtain DiB2‐split protein

   Depositor

   Jens Meiler

   Article

   Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10

   Insert

   N-fragment of DiB2
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSADuet148

   Plasmid

   #121910

   Purpose

   Charge-introduced NpuDnaBΔ283ΔC39 intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   CI-NpuDnaBΔ290ΔC39 intein
   

   Use

   Tags

   His6-GB1

   Expression

   Bacterial

   Mutation

   Deletion of 290-residue endonuclease domain, I53K…

   Promoter

   T7

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHBDuet259

   Plasmid

   #121911

   Purpose

   NpuDnaBΔ283ΔC39 intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   NpuDnaBΔ283ΔC39 intein
   

   Use

   Tags

   His6-GB1

   Expression

   Bacterial

   Mutation

   Deletion of 283-residue endonuclease domain

   Promoter

   T7

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHBBAD113

   Plasmid

   #121912

   Purpose

   Charge-Introduced NpuDnaBC39 intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   CI-NpuDnaBC39 intein
   

   Use

   Tags

   GB1-His6

   Expression

   Bacterial

   Mutation

   S110K, I111K

   Promoter

   Para

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHBDuet116

   Plasmid

   #121915

   Purpose

   NpuDnaBΔ290ΔC39 intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   Oth-NpuDnaBΔ290ΔC39 intein
   

   Use

   Tags

   His6-GB1

   Expression

   Bacterial

   Mutation

   Deletion of 290-residue endonuclease domain, I53K…

   Promoter

   T7

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHBBAD168

   Plasmid

   #121916

   Purpose

   Oth-NpuDnaBC39 intein

   Depositor

   Hideo Iwai

   Article

   Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

   Insert

   Oth-NpuDnaBC39 intein
   

   Use

   Tags

   GB1-His6

   Expression

   Bacterial

   Mutation

   I111K, E116K

   Promoter

   Para

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HA-Cer-NCreIntN

   Plasmid

   #160505

   Purpose

   Expresses NCreIntN, tagged with HA, in mammalian cells in split intein-mediated split-Cre recombinase applications

   Depositor

   Shawn Je

   Article

   Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

   Insert

   NCreIntN
   

   Use

   Cre/Lox

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FLAG-IntCCCre

    Plasmid

    #160506

    Purpose

    Expresses IntCCCre, tagged with FLAG, in mammalian cells in split intern-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    IntCCCre
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET11a-Z-N-DiB2

    Plasmid

    #168473

    Purpose

    N-fragment of the DiB2‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain DiB2‐split-Zip protein

    Depositor

    Jens Meiler

    Article

    Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10

    Insert

    N-fragment of DiB2 + Leucine Zipper
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHBDuet139

    Plasmid

    #121913

    Purpose

    Charge-Introduced NpuDnaB mini-intein

    Depositor

    Hideo Iwai

    Article

    Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

    Insert

    CI-NpuDnaBΔ290 intein
    

    Use

    Tags

    GB1 and His6-GB1

    Expression

    Bacterial

    Mutation

    Deletion of 290-residue endonuclease domain, I53K…

    Promoter

    T7

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHBDuet140

    Plasmid

    #121914

    Purpose

    Orthogonal NpuDnaB mini-intein intein

    Depositor

    Hideo Iwai

    Article

    Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

    Insert

    Oth-NpuDnaBΔ290 intein
    

    Use

    Tags

    GB1 and His6-GB1

    Expression

    Bacterial

    Mutation

    Deletion of 290-residue endonuclease domain, I53K…

    Promoter

    T7

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDH-CAG-IntCCCre-T2A-CopGFP

    Plasmid

    #160501

    Purpose

    Expresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    IntCCCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    CopGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDH-hSyn-NCreIntN-T2A-mTagBFP2

    Plasmid

    #160502

    Purpose

    Expresses NCreIntN in neurons in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    NCreIntN
    

    Use

    Cre/Lox and Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDH-CAG-NCreIntN-T2A-mTagBFP2

    Plasmid

    #160500

    Purpose

    Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    NCreIntN
    

    Use

    Cre/Lox and Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDH-CAG-NCreIntN-EF1a-mTagBFP2

    Plasmid

    #160507

    Purpose

    Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    NCreIntN
    

    Use

    Cre/Lox and Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDH-hSyn-IntCCCre-T2A-CopGFP

    Plasmid

    #160503

    Purpose

    Expresses IntCCCre in neurons in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    IntCCCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    CopGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    March 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDH-CAG-IntCCCre-EF1a-Cerulean

    Plasmid

    #160508

    Purpose

    Expresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    IntCCCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28b_H6-Smt3-AesC-GFP

    Plasmid

    #234486

    Purpose

    Superfolder GFP C-terminally fused to His-Smt3-tagged AesC split intein

    Depositor

    Henning Mootz

    Article

    Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

    Insert

    C-terminal Fragment of the Aes123 PolB1 Split Intein
    

    Use

    Tags

    Hexahistidine Tag, Superfolder Green Fluorescent …

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge