We narrowed to 3,398 results for: aaas

1. HA, EGFP Spastin M87 with exon 4, N386C

   Plasmid

   #167543

   Purpose

   For doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4, with N386C mutation.

   Depositor

   Tarun Kapoor

   Article

   Cupido et al Nat Chem Biol. 2019 May;15(5):444-452. d

   Insert

   Spastin M87 with exon 4 (SPAST Human)
   

   Tags

   EGFP and HA tag

   Expression

   Mammalian

   Mutation

   N386C

   Promoter

   CMV

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SNAP-MIDAS

   Plasmid

   #171604

   Purpose

   Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) in bacteria.

   Depositor

   Tarun Kapoor

   Article

   Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

   Insert

   MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
   

   Tags

   His6 tag and SNAP tag

   Expression

   Bacterial

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. KBB430

   Plasmid

   #185091

   Purpose

   NMA111-GFP with first nuclear localization signal mutated (amino acids 28 - 30, KRK -> AAA)

   Depositor

   Ken Belanger

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NMA111
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   First nuclear localization signal mutated (amino …

   Available Since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. KBB428

   Plasmid

   #185090

   Purpose

   NMA111-GFP with first nuclear localization signal mutated (amino acids 9 - 11, KKR -> AAA)

   Depositor

   Ken Belanger

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NMA111
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   First nuclear localization signal mutated (amino …

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Mdn1-deltaC

   Plasmid

   #171603

   Purpose

   Expresses S. pombe Mdn1 a.a. 1-3911 in insect cells.

   Depositor

   Tarun Kapoor

   Article

   Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

   Insert

   Mdn1
   

   Tags

   His6 tag

   Expression

   Insect

   Mutation

   C-terminal deletion; a.a. 1-3911 only

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFL His-SDS22

   Plasmid

   #113512

   Purpose

   Expression of His-SDS22 in insect cells (Baculovirus system)

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   SDS22 (PPP1R7 Human)
   

   Tags

   6*His

   Expression

   Insect

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. p36-p37-p38-p40-pET-Duet-1

   Plasmid

   #175049

   Purpose

   Overexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coli

   Depositor

   Brian Kelch

   Article

   Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   Replication factor C subunits 2-5 (RFC4, RFC2, RFC3, RFC5 Human)
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET41b+_Npl4

   Plasmid

   #117108

   Purpose

   Production of recombinant Npl4 in E.coli

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   Npl4 (NPLOC4 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-6P-1 p37deltaSEP

   Plasmid

   #113501

   Purpose

   Bacterial expression of GST-tagged p37 lacking the SEP domain

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p37 (Ubxn2b Mouse)
   

   Tags

   GST-PreScission protease site

   Expression

   Bacterial

   Mutation

   delta 140-206

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET41b+_Ufd1-His

    Plasmid

    #117107

    Purpose

    Production of recombinant Ufd1-His in E.coli

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    Ufd1 (UFD1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-BLM DM

    Plasmid

    #80071

    Purpose

    Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

    Depositor

    Nathan Ellis

    Article

    Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

    Insert

    BLM (BLM Human)
    

    Tags

    GFP-BLM

    Expression

    Mammalian

    Mutation

    K317R (AAA>AGA) and K331R (AAA>AGA)

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-4T-1 UBXN2A

    Plasmid

    #113505

    Purpose

    Bacterial expression of GST-tagged UBXN2A

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    UBXN2A (UBXN2A Human)
    

    Tags

    GST-Thrombin cleavage site

    Expression

    Bacterial

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFL His-SDS22 / PP1gamma

    Plasmid

    #113511

    Purpose

    Expression of His-SDS22 / PP1gamma dimer in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Inserts

    SDS22 (PPP1R7 Human)
    PP1gamma (PPP1CC Human)
    

    Tags

    6*His

    Expression

    Insect

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFL His-mEos3.2-I3

    Plasmid

    #113516

    Purpose

    Expression of His-Eos-I3 in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    I3 (PPP1R11 Human)
    

    Tags

    6*His-mEos3.2

    Expression

    Insect

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-6P-1 p37

    Plasmid

    #113500

    Purpose

    Bacterial expression of GST-tagged p37

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. UbG76V-eGFP-V5His_15aa

    Plasmid

    #23969

    Depositor

    Nico Dantuma

    Article

    Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

    Insert

    Ub-G76V-eGFP-V5HIS_15aa (UBB Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    G76V mutation in Ubiquitin. Ub fused to N-termin…

    Available Since

    April 16, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5FRT/TO PP1gamma-2*Strep

    Plasmid

    #113506

    Purpose

    Expresses dual Strep-tagged PP1gamma in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    PP1gamma (PPP1CC Human)
    

    Tags

    Strep-Strep

    Expression

    Mammalian

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-PODXL FBM*

    Plasmid

    #195220

    Purpose

    Mammalian expression vector containing GFP tagged Podocalyxin. With H477/R479/S481>AAA mutation.

    Depositor

    Keith Mostov, David Bryant

    Article

    Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

    Insert

    PODXL (PODXL Rabbit)
    

    Expression

    Mammalian

    Mutation

    H477/R479/S481>AAA

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-PODXL FBM*/PBM*

    Plasmid

    #195221

    Purpose

    Mammalian expression vector containing GFP tagged Podocalyxin. Dual mutant for HRS>AAA / lacking DTHL.

    Depositor

    Keith Mostov, David Bryant

    Article

    Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

    Insert

    PODXL (PODXL Rabbit)
    

    Expression

    Mammalian

    Mutation

    Dual mutant for HRS>AAA / lacking DTHL

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. deltaLoop-MIDAS

    Plasmid

    #171605

    Purpose

    Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) without the loop region (a.a. 4458-4496) in bacteria.

    Depositor

    Tarun Kapoor

    Article

    Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

    Insert

    MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
    

    Tags

    His6 tag and SNAP tag

    Expression

    Bacterial

    Mutation

    Replaced amino acids 4458-4496 with ASGSGS linker

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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